【摘要】： 蛋白質(zhì)是生命的物質(zhì)基礎(chǔ),沒有蛋白質(zhì)就沒有生命。它是與生命、與各種形式的生命活動(dòng)緊密聯(lián)系在一起的物質(zhì)。組成蛋白質(zhì)的基本單位是氨基酸,氨基酸是構(gòu)建生物機(jī)體的眾多生物活性大分子之一,是構(gòu)建細(xì)胞、修復(fù)組織的基礎(chǔ)材料。腎上腺素是哺乳動(dòng)物和人類的一種重要的兒茶酚胺類神經(jīng)傳遞物質(zhì),它控制著神經(jīng)系統(tǒng)進(jìn)行一系列生物反應(yīng)及神經(jīng)化學(xué)過程。研究氨基酸分子與腎上腺素的相互作用,對于推動(dòng)生命科學(xué)、藥物化學(xué)、物理化學(xué)等學(xué)科的發(fā)展能起到一定的積極作用。本文用電化學(xué)方法研究了甘氨酸、絲氨酸、谷氨酸、天冬氨酸、苯丙氨酸五種氨基酸對腎上腺素電化學(xué)氧化的影響,著重討論了苯丙氨酸對腎上腺素電化學(xué)行為的影響,并輔助于量子化學(xué)方法對苯丙氨酸與腎上腺素形成的氫鍵復(fù)合物進(jìn)行了理論探討。 首先,利用循環(huán)伏安法研究了甘氨酸、絲氨酸、谷氨酸、天冬氨酸、苯丙氨酸在鹽酸體系中對腎上腺素電子轉(zhuǎn)移性質(zhì)的影響,實(shí)驗(yàn)結(jié)果表明:這幾種氨基酸對腎上腺素具有一定的穩(wěn)定作用,能在一定程度上抑制腎上腺素的氧化,從而改變了腎上腺素的循環(huán)伏安特征,并且氨基酸的結(jié)構(gòu)對腎上腺素的氧化能力有一定影響。鹽酸溶液中,不同結(jié)構(gòu)的氨基酸抑制腎上腺素氧化的能力順序?yàn)?甘氨酸＜絲氨酸＜谷氨酸＜天冬氨酸＜苯丙氨酸。 其次,選用不同pH、不同的溶液組成及鉑電極、玻碳電極,利用循環(huán)伏安法研究了苯丙氨酸在鹽酸體系中對。腎上腺素電子轉(zhuǎn)移性質(zhì)的影響。實(shí)驗(yàn)結(jié)果表明:在不同pH的鹽酸體系、鹽酸.氯化鈉緩沖溶液體系、B.R.緩沖溶液以及pH=7.4的生理介質(zhì)KRPB中,苯丙氨酸均對腎上腺素具有一定的穩(wěn)定作用,能在一定程度上抑制腎上腺素的氧化。隨著pH的增大、苯丙氨酸濃度的增大,腎上腺素氧化峰電位正移,還原峰電位負(fù)移,峰電流減小,腎上腺素的循環(huán)伏安特征以此發(fā)生了改變。 最后,用量子化學(xué)方法中的DFT-B3LYP方法對苯丙氨酸與腎上腺素分子間的氫鍵作用作了理論研究,優(yōu)化了苯丙氨酸與腎上腺素形成氫鍵復(fù)合物的幾何構(gòu)型,對形成的各種氫鍵復(fù)合物通過比較成鍵前后的一系列參數(shù)進(jìn)行了理論探討。氣相中苯丙氨酸能與腎上腺素形成9種1:1氫鍵復(fù)合物,液相中則是形成5種。氫鍵的形成對腎上腺素的羥基起到了保護(hù)作用,使其苯環(huán)上相鄰的羥基(-OH)上的H難以脫去,降低了體系的能量,增加了腎上腺素的穩(wěn)定性,在一定程度上抑制了腎上腺素的氧化,并且結(jié)合結(jié)構(gòu)參數(shù)和能量,解釋了有關(guān)實(shí)驗(yàn)現(xiàn)象。
[Abstract]:Protein is the material foundation of life, without which there is no life. It is a substance closely associated with life, with all forms of life. The basic unit of protein is amino acid, which is one of the bioactive macromolecules in the construction of biological organism. It is the basic material to construct cells and repair tissues. Epinephrine is an important catecholamine neurotransmitter in mammals and humans. It controls a series of biological reactions and neurochemical processes in the nervous system. The study of the interaction between amino acid molecules and epinephrine can play a positive role in promoting the development of life science, drug chemistry, physical chemistry and so on. The effects of glycine, serine, glutamic acid, aspartic acid and phenylalanine on the electrochemical oxidation of epinephrine were studied by electrochemical method. The hydrogen bond complex formed by phenylalanine and epinephrine was studied theoretically by means of quantum chemical method. Firstly, the effects of glycine, serine, glutamate, aspartic acid and phenylalanine on the electron transfer properties of epinephrine in hydrochloric acid system were studied by cyclic voltammetry. The results show that these amino acids can stabilize epinephrine to a certain extent and inhibit the oxidation of epinephrine to a certain extent, thus changing the cyclic voltammetry of epinephrine. The structure of amino acids has a certain effect on the oxidation ability of epinephrine. In hydrochloric acid solution, the order of the ability of amino acids with different structures to inhibit the oxidation of epinephrine was glycine < serine < glutamic acid < aspartic acid < phenylalanine. Secondly, phenylalanine in hydrochloric acid system was studied by cyclic voltammetry with different pH, solution composition, platinum electrode and glassy carbon electrode. The effect of epinephrine electron transfer properties. The results show that in different pH hydrochloric acid systems, hydrochloric acid. Sodium chloride buffer solution system, B.R. In buffer solution and KRPB, the physiological medium of pH=7.4, phenylalanine can stabilize epinephrine to some extent and inhibit the oxidation of epinephrine to some extent. With the increase of pH, the concentration of phenylalanine increases, the peak potential of epinephrine oxidation is shifted positively, the peak potential of reduction shifts negatively, the peak current decreases, and the cyclic voltammetry of epinephrine changes. Finally, the hydrogen bond between phenylalanine and epinephrine was studied theoretically by the DFT-B3LYP method in quantum chemistry, and the geometric configuration of the hydrogen bond complex between phenylalanine and epinephrine was optimized. A series of parameters before and after bonding are discussed theoretically. Phenylalanine can form nine 1:1 hydrogen bond complexes with epinephrine in gas phase and 5 in liquid phase. The formation of hydrogen bond protects the hydroxyl group of epinephrine, which makes it difficult to remove H from the adjacent hydroxyl group (- OH) on the benzene ring, which decreases the energy of the system and increases the stability of epinephrine. The oxidation of epinephrine was inhibited to some extent, and the experimental phenomena were explained by combining the structural parameters and energy.
【學(xué)位授予單位】：曲阜師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R341

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳銀霞;;氨基酸在醫(yī)藥及工業(yè)中的應(yīng)用[J];才智;2008年17期

2 趙殊,劉本才,廖蓉蘇;氨基酸在水溶液中的行為[J];東北林業(yè)大學(xué)學(xué)報(bào);2004年04期

3 汪振輝,張永花,周漱萍;聚吖啶橙修飾電極的電化學(xué)行為及其對腎上腺素的電催化性能[J];分析化學(xué);2001年12期

4 靳桂英,張玉忠,楊周生;聚氨基磺酸修飾玻碳電極在抗壞血酸共存時(shí)測定腎上腺素[J];分析化學(xué);2005年01期

5 郁章玉,秦梅,張為超,翟翠萍,汪漢卿;冰醋酸中腎上腺素的現(xiàn)場光譜電化學(xué)行為[J];分析化學(xué);2005年01期

6 馬偉;孫登明;;聚L-精氨酸修飾電極存在下同時(shí)測定多巴胺和腎上腺素[J];分析化學(xué);2007年01期

7 張翼;楊槞;;氫鍵研究進(jìn)展[J];化學(xué)教育;2007年02期

8 張國梅,雙少敏,鈔建賓,潘景浩;環(huán)糊精超分子化學(xué)在生命科學(xué)研究中的新進(jìn)展[J];分析科學(xué)學(xué)報(bào);2005年02期

9 汪振輝,劉小花,張岱,周漱萍;腎上腺素在聚對氨基吡啶化學(xué)修飾電極上的電化學(xué)行為及其吸附伏安法測定[J];分析試驗(yàn)室;2003年05期

10 馬心英;王朝霞;;腎上腺素在聚L-半胱氨酸修飾玻碳電極上的電化學(xué)行為[J];分析試驗(yàn)室;2008年01期

，

本文編號：2294328