【摘要】：背景:隨著對脊髓損傷研究的不斷深入,國內(nèi)外學者不斷設(shè)計出各種脊髓損傷模型,主要包括脊髓挫傷、重物墜擊、脊髓壓迫、化學灼傷、放射性損傷及激素橫斷半橫斷損傷等,然而各種制備方法都存在一定的局限。目的:設(shè)計脊髓損傷致傷器并建立不同程度脊髓損傷動物模型。方法:實驗自行設(shè)計包括致傷部件及傳動裝置的脊髓損傷致傷器,利用不同致傷質(zhì)量m1=10 g,m2=20 g,m3=30 g及時間T1=3 s,T2=5 s,造成不同質(zhì)量×時間暨不同程度SD大鼠脊髓背側(cè)壓迫損傷,分為m1T1,m2T1,m3T1,m1T2,m2T2,m3T2共6組,并設(shè)假手術(shù)組進行對照。結(jié)果與結(jié)論:造模后各實驗組BBB評分均低于假手術(shù)組(P0.01),m1T1組與其他各實驗組相比差異有顯著性意義(P0.01),建模后8周m1T2組BBB評分高于與m2T2組及m3T2組(P0.05)。建模后8周各組體感誘發(fā)電位和運動誘發(fā)電位潛伏期明顯長于假手術(shù)組(P0.01)。除了m1T1組與m1T2組外,其他各組間建模后體感誘發(fā)電位潛伏期均明顯延長(P0.05)。所有組建模后運動誘發(fā)電位潛伏期均明顯延長(P0.05)。結(jié)果證實,實驗所設(shè)計的脊髓損傷致傷器可制備出不同程度的脊髓背側(cè)壓迫損傷動物模型。
[Abstract]:Background: with the development of the research on spinal cord injury, scholars at home and abroad have designed various spinal cord injury models, including spinal cord contusion, heavy weight drop, spinal cord compression, chemical burns. However, there are some limitations in various preparation methods, such as radiation injury and hormone transection injury. Objective: to design a spinal cord injury apparatus and to establish animal models of spinal cord injury. Methods: the spinal cord injury apparatus including injured parts and transmission device was designed by ourselves. The spinal cord injury was induced by using different injury mass of 10 g / m ~ (2) and 20 g / m ~ (3) C ~ (30 g) and time T _ (1) ~ (3) S ~ (3) T _ (2) + T _ (2) ~ (5) s to induce spinal cord dorsal compression injury in different quality 脳 time and different degree of SD rats. The patients were divided into 6 groups: m1T1, m2T1, m3T1, m1T2, m2T2m2m3T2, and sham-operated group. Results and conclusion: the BBB score of each experimental group was lower than that of sham operation group (P0.01), and the BBB score of m1T2 group was higher than that of m2T2 group and m3T2 group at 8 weeks after modeling (P0.01), and there was significant difference between m1T1 group and other experimental groups (P0.01). The latency of somatosensory evoked potential and motor evoked potential in each group was significantly longer than that in sham operation group at 8 weeks after modeling (P0.01). The latency of somatosensory evoked potential (SEP) was significantly prolonged after modeling between m1T1 group and m1T2 group (P0.05). After modeling, the latency of motor evoked potential was significantly prolonged in all groups (P0.05). The results show that the spinal cord injury apparatus designed by the experiment can produce the animal model of spinal cord dorsal compression injury with different degrees.
【作者單位】： 天津醫(yī)科大學總醫(yī)院骨科;內(nèi)蒙古醫(yī)科大學第二附屬醫(yī)院康復科;
【分類號】：R651.2;R-332

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