發(fā)布時(shí)間：2018-10-21 18:33

【摘要】：背景:隨著對(duì)脊髓損傷研究的不斷深入,國(guó)內(nèi)外學(xué)者不斷設(shè)計(jì)出各種脊髓損傷模型,主要包括脊髓挫傷、重物墜擊、脊髓壓迫、化學(xué)灼傷、放射性損傷及激素橫斷半橫斷損傷等,然而各種制備方法都存在一定的局限。目的:設(shè)計(jì)脊髓損傷致傷器并建立不同程度脊髓損傷動(dòng)物模型。方法:實(shí)驗(yàn)自行設(shè)計(jì)包括致傷部件及傳動(dòng)裝置的脊髓損傷致傷器,利用不同致傷質(zhì)量m1=10 g,m2=20 g,m3=30 g及時(shí)間T1=3 s,T2=5 s,造成不同質(zhì)量×?xí)r間暨不同程度SD大鼠脊髓背側(cè)壓迫損傷,分為m1T1,m2T1,m3T1,m1T2,m2T2,m3T2共6組,并設(shè)假手術(shù)組進(jìn)行對(duì)照。結(jié)果與結(jié)論:造模后各實(shí)驗(yàn)組BBB評(píng)分均低于假手術(shù)組(P0.01),m1T1組與其他各實(shí)驗(yàn)組相比差異有顯著性意義(P0.01),建模后8周m1T2組BBB評(píng)分高于與m2T2組及m3T2組(P0.05)。建模后8周各組體感誘發(fā)電位和運(yùn)動(dòng)誘發(fā)電位潛伏期明顯長(zhǎng)于假手術(shù)組(P0.01)。除了m1T1組與m1T2組外,其他各組間建模后體感誘發(fā)電位潛伏期均明顯延長(zhǎng)(P0.05)。所有組建模后運(yùn)動(dòng)誘發(fā)電位潛伏期均明顯延長(zhǎng)(P0.05)。結(jié)果證實(shí),實(shí)驗(yàn)所設(shè)計(jì)的脊髓損傷致傷器可制備出不同程度的脊髓背側(cè)壓迫損傷動(dòng)物模型。
[Abstract]:Background: with the development of the research on spinal cord injury, scholars at home and abroad have designed various spinal cord injury models, including spinal cord contusion, heavy weight drop, spinal cord compression, chemical burns. However, there are some limitations in various preparation methods, such as radiation injury and hormone transection injury. Objective: to design a spinal cord injury apparatus and to establish animal models of spinal cord injury. Methods: the spinal cord injury apparatus including injured parts and transmission device was designed by ourselves. The spinal cord injury was induced by using different injury mass of 10 g / m ~ (2) and 20 g / m ~ (3) C ~ (30 g) and time T _ (1) ~ (3) S ~ (3) T _ (2) + T _ (2) ~ (5) s to induce spinal cord dorsal compression injury in different quality 脳 time and different degree of SD rats. The patients were divided into 6 groups: m1T1, m2T1, m3T1, m1T2, m2T2m2m3T2, and sham-operated group. Results and conclusion: the BBB score of each experimental group was lower than that of sham operation group (P0.01), and the BBB score of m1T2 group was higher than that of m2T2 group and m3T2 group at 8 weeks after modeling (P0.01), and there was significant difference between m1T1 group and other experimental groups (P0.01). The latency of somatosensory evoked potential and motor evoked potential in each group was significantly longer than that in sham operation group at 8 weeks after modeling (P0.01). The latency of somatosensory evoked potential (SEP) was significantly prolonged after modeling between m1T1 group and m1T2 group (P0.05). After modeling, the latency of motor evoked potential was significantly prolonged in all groups (P0.05). The results show that the spinal cord injury apparatus designed by the experiment can produce the animal model of spinal cord dorsal compression injury with different degrees.
【作者單位】： 天津醫(yī)科大學(xué)總醫(yī)院骨科;內(nèi)蒙古醫(yī)科大學(xué)第二附屬醫(yī)院康復(fù)科;
【分類(lèi)號(hào)】：R651.2;R-332

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 尚云龍;李一帆;寧云峰;呂元石;李曉霜;趙杰;李聰聰;;大鼠脊髓損傷模型的解剖學(xué)參考定位[J];解剖學(xué)研究;2013年06期

2 李思偉;王素彬;張志華;;骨髓間充質(zhì)干細(xì)胞治療脊髓損傷的研究進(jìn)展[J];創(chuàng)傷外科雜志;2014年04期

3 趙巖;肖宇龍;左媛;王喜良;霍洪軍;江建明;閆慧博;;神經(jīng)干細(xì)胞移植聯(lián)合促紅細(xì)胞生成素對(duì)大鼠脊髓損傷的修復(fù)作用[J];吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2015年02期

4 吳岳嵩;柳霞;李茜;張安仁;喬晉琳;;康復(fù)醫(yī)學(xué)新進(jìn)展與發(fā)展設(shè)想[J];解放軍醫(yī)學(xué)雜志;2010年05期

5 蔣戈利;;康復(fù)醫(yī)學(xué)發(fā)展趨勢(shì)及設(shè)想[J];解放軍醫(yī)藥雜志;2013年01期

6 陳祥;余資江;;電針與骨髓間充質(zhì)干細(xì)胞移植在脊髓損傷修復(fù)及再生中的作用[J];局解手術(shù)學(xué)雜志;2013年02期

7 李曰眾;劉亞;魏偉;;依那西普治療脊髓損傷的臨床和神經(jīng)電生理觀(guān)察[J];遼寧醫(yī)學(xué)院學(xué)報(bào);2014年01期

8 芮碧宇;曾炳芳;王金武;郭尚春;陳欣;;Using the Implantable Electrical Stimulator for Peripheral Nerve Rehabilitation:A Study in an Animal Model[J];Journal of Shanghai Jiaotong University(Science);2009年05期

9 Ruiping Zhang;Kun Zhang;Jianding Li;Qiang Liu;Jun Xie;;In vivo tracking of neuronal-like cells by magnetic resonance in rabbit models of spinal cord injury[J];Neural Regeneration Research;2013年36期

10 董賢慧;高維娟;;脊髓損傷動(dòng)物模型建立方法研究進(jìn)展[J];神經(jīng)解剖學(xué)雜志;2014年01期

相關(guān)會(huì)議論文 前1條

1 周永全;李強(qiáng);張寧;;脊髓型頸椎病動(dòng)物模型的研究近況[A];2012年中國(guó)藥學(xué)大會(huì)暨第十二屆中國(guó)藥師周論文集[C];2012年

相關(guān)博士學(xué)位論文 前10條

1 盧燕;少突膠質(zhì)細(xì)胞在中樞神經(jīng)發(fā)育與再生中的作用機(jī)制研究[D];第二軍醫(yī)大學(xué);2013年

2 陳智;p62在大鼠慢性脊髓損傷中的作用機(jī)制研究[D];第二軍醫(yī)大學(xué);2013年

3 茅磊;調(diào)控Nrf2-ARE通路對(duì)小鼠脊髓損傷治療作用的實(shí)驗(yàn)研究[D];南京大學(xué);2011年

4 錢(qián)軍;振蕩電場(chǎng)刺激對(duì)脊髓少突膠質(zhì)前體細(xì)胞的活化機(jī)制研究[D];蘇州大學(xué);2014年

5 李經(jīng)輝;電針對(duì)大鼠脊髓損傷后內(nèi)源性神經(jīng)干細(xì)胞分化與BMP-2表達(dá)的實(shí)驗(yàn)研究[D];昆明醫(yī)科大學(xué);2013年

6 荀，

本文編號(hào)：2285989