雌激素制備大鼠子宮肌瘤動物模型
發(fā)布時間：2018-10-04 21:25
【摘要】：[背景]子宮肌瘤是女性生殖系統(tǒng)中最常見的一種良性腫瘤,目前對于子宮肌瘤的發(fā)病機(jī)制尚不十分清楚。資料表明雌激素是肌瘤生長的主要促進(jìn)因素。目前尚缺乏適宜的動物模型可供臨床發(fā)病機(jī)制研究及藥物篩選,建模的給藥劑量及給藥周期尚存在爭議,值得進(jìn)一步探索。 [目的]建立適合于臨床發(fā)病機(jī)制研究和藥物篩選的子宮肌瘤動物模型。 [方法]選取性成熟大鼠,隨機(jī)分組給予200μg和300μg劑量雌激素(E2)誘導(dǎo),并于8wk和10wk時處死部分動物,測定血清中雌激素(E2)和孕激素(P)的水平,觀察子宮形態(tài)的變化,計算肌瘤形成率和動物死亡率,并與人子宮肌瘤進(jìn)行病理組織學(xué)特征的比較,同時測定組織中雌、孕激素受體(ER、PR)的表達(dá)。確定E2誘導(dǎo)大鼠子宮肌瘤形成的最佳劑量和給藥周期。 [結(jié)果]肌肉注射E2200μg只,2次/wk,連續(xù)8wk后,大鼠血清E2水平顯著升高,子宮重量和臟器系數(shù)增加。大鼠子宮明顯變形,肌層增生,部分大鼠肉眼可見漿膜下肌瘤或肌壁間肌瘤形成。病理組織學(xué)檢查顯示,肌瘤細(xì)胞與人子宮肌瘤病理組織學(xué)特征相似,肌瘤組織中雌、孕激素受體(ER, PR)表達(dá)明顯增強(qiáng)。 [結(jié)論]大鼠肌肉注射E2200μt/只,2次/wk,連續(xù)8wk后可建立與人相似的子宮肌瘤動物模型,適合用于臨床發(fā)病機(jī)制研究和藥物篩選。
[Abstract]:Background: uterine leiomyoma is the most common benign tumor in female reproductive system. The data show that estrogen is the main promoting factor of leiomyoma growth. At present, there is a lack of suitable animal models for clinical pathogenesis research and drug screening. The amount and cycle of drug administration in the model is still controversial, which is worth further exploration. Objective to establish an animal model of uterine leiomyoma suitable for clinical pathogenesis research and drug screening. [methods] sexual mature rats were randomly divided into two groups: 200 渭 g and 300 渭 g doses of estrogen (E _ 2). Some animals were killed at 8wk and 10wk. The levels of estrogen (E _ 2) and progesterone (P) in serum were measured, and the changes of uterine morphology were observed. The rate of leiomyoma formation and animal mortality were calculated, and the histopathological characteristics of human uterine leiomyoma were compared. The expression of estrogen and progesterone receptor (ER,PR) in human uterine leiomyoma was also determined. To determine the optimal dose and administration period of E2-induced hysteromyoma formation in rats. [results] after intramuscular injection of E2200 渭 g for 2 times / wk, serum E2 level, uterine weight and organ coefficient increased significantly after continuous 8wk. The uterus was obviously deformed and myometrium proliferated. Subserous myoma or myomyoma could be seen in the naked eyes of some rats. Histopathological examination showed that the histopathological features of leiomyoma cells were similar to those of human uterine leiomyoma. The expression of estrogen and progesterone receptor (ER, PR) was significantly increased in leiomyoma tissues. [conclusion] after intramuscular injection of E2200 渭 t / L / 2 wk, the rat model of uterine leiomyoma similar to human can be established after continuous 8wk, which is suitable for clinical pathogenesis research and drug screening.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級別】：博士
【學(xué)位授予年份】：2009
【分類號】：R-332;R737.33
本文編號：2251926
[Abstract]:Background: uterine leiomyoma is the most common benign tumor in female reproductive system. The data show that estrogen is the main promoting factor of leiomyoma growth. At present, there is a lack of suitable animal models for clinical pathogenesis research and drug screening. The amount and cycle of drug administration in the model is still controversial, which is worth further exploration. Objective to establish an animal model of uterine leiomyoma suitable for clinical pathogenesis research and drug screening. [methods] sexual mature rats were randomly divided into two groups: 200 渭 g and 300 渭 g doses of estrogen (E _ 2). Some animals were killed at 8wk and 10wk. The levels of estrogen (E _ 2) and progesterone (P) in serum were measured, and the changes of uterine morphology were observed. The rate of leiomyoma formation and animal mortality were calculated, and the histopathological characteristics of human uterine leiomyoma were compared. The expression of estrogen and progesterone receptor (ER,PR) in human uterine leiomyoma was also determined. To determine the optimal dose and administration period of E2-induced hysteromyoma formation in rats. [results] after intramuscular injection of E2200 渭 g for 2 times / wk, serum E2 level, uterine weight and organ coefficient increased significantly after continuous 8wk. The uterus was obviously deformed and myometrium proliferated. Subserous myoma or myomyoma could be seen in the naked eyes of some rats. Histopathological examination showed that the histopathological features of leiomyoma cells were similar to those of human uterine leiomyoma. The expression of estrogen and progesterone receptor (ER, PR) was significantly increased in leiomyoma tissues. [conclusion] after intramuscular injection of E2200 渭 t / L / 2 wk, the rat model of uterine leiomyoma similar to human can be established after continuous 8wk, which is suitable for clinical pathogenesis research and drug screening.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級別】：博士
【學(xué)位授予年份】：2009
【分類號】：R-332;R737.33
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，本文編號：2251926
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