三種類型的蘇木精在HE染色中的效果比較
發(fā)布時間:2018-09-08 07:19
【摘要】:正自1863年Waldeyer倡導(dǎo)使用蘇木精染色細(xì)胞核,伊紅染色細(xì)胞質(zhì),使不同組織和細(xì)胞成分呈現(xiàn)不同顏色和產(chǎn)生不同的折射率,HE染色一直被廣泛應(yīng)用于病理形態(tài)學(xué)診斷、教學(xué)和研究。在實際工作中使用的蘇木精通常為自然氧化或人工氧化兩種,將自然氧化和人工氧化的蘇木精以1∶1比例混合成混合蘇木精,染色效果很好,現(xiàn)將這三種類型的蘇木精在HE染色中的效果介紹如下。
[Abstract]:Since 1863 Waldeyer advocated the use of hematoxylin staining of nucleus and eosin staining of cytoplasm, which makes different tissues and cell components present different colors and produces different refractive index HE staining. It has been widely used in pathological morphology diagnosis, teaching and research. The hematoxylin used in practical work is usually natural oxidation or artificial oxidation. The mixture of natural and artificially oxidized hematoxylin is mixed into hematoxylin at 1:1, and the dyeing effect is very good. The effects of these three types of hematoxylin in HE dyeing are described below.
【作者單位】: 武漢市中心醫(yī)院病理科;武漢大學(xué)人民醫(yī)院病理科;
【基金】:湖北省自然科學(xué)基金課題(2013CFB374) 湖北省武漢市衛(wèi)生局立項課題(WX12C58)
【分類號】:R361.2
本文編號:2229781
[Abstract]:Since 1863 Waldeyer advocated the use of hematoxylin staining of nucleus and eosin staining of cytoplasm, which makes different tissues and cell components present different colors and produces different refractive index HE staining. It has been widely used in pathological morphology diagnosis, teaching and research. The hematoxylin used in practical work is usually natural oxidation or artificial oxidation. The mixture of natural and artificially oxidized hematoxylin is mixed into hematoxylin at 1:1, and the dyeing effect is very good. The effects of these three types of hematoxylin in HE dyeing are described below.
【作者單位】: 武漢市中心醫(yī)院病理科;武漢大學(xué)人民醫(yī)院病理科;
【基金】:湖北省自然科學(xué)基金課題(2013CFB374) 湖北省武漢市衛(wèi)生局立項課題(WX12C58)
【分類號】:R361.2
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1 林志雄;蘇木素染液快速配制法[J];臨床與實驗病理學(xué)雜志;1992年03期
,本文編號:2229781
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