【摘要】： 目的:探討青春期酒精刺激對大鼠成年后學(xué)習(xí)記憶與海馬結(jié)構(gòu)突觸囊泡素表達的影響 方法:取青春期40只SD大鼠分為對照組與實驗組,兩組動物分別以生理鹽水、2g/kg酒精連續(xù)注射5天,每天固定時間2次,然后正常飼養(yǎng),待成年后用Morris水迷宮對各組大鼠進行空間辨別性學(xué)習(xí)記憶的測試,用攝像機拍攝大鼠的行為,再用統(tǒng)計分析軟件分析水迷宮檢測結(jié)果。用免疫組織化學(xué)方法檢測正常對照組與實驗組大鼠海馬結(jié)構(gòu)突觸囊泡素的表達。 結(jié)果:Morris水迷宮結(jié)果:各組大鼠隨著訓(xùn)練次數(shù)的增加逃避潛伏期(EL)所用時間逐漸縮短。酒精組逃避潛伏期(EL)比正常對照組長,差異有統(tǒng)計學(xué)意義(P＜0.05)。 突觸囊泡素免疫表達:突觸囊泡素的免疫表達主要集中表達在海馬結(jié)構(gòu)的CA_3以及齒狀回,為褐色顆粒狀,呈板狀分布。正常對照組與酒精組比較,大鼠海馬結(jié)構(gòu)CA_3跟齒狀回突觸囊泡素免疫反應(yīng)陽性產(chǎn)物的平均灰度值差異有統(tǒng)計學(xué)意義(P＜0.05)。 結(jié)論:青春期接受酒精刺激的大鼠,成年后海馬結(jié)構(gòu)CA_3、齒狀回突觸囊泡素表達減少,Morris水迷宮測試中的逃避潛伏期延長,空間學(xué)習(xí)與記憶能力下降。
[Abstract]:Objective: to investigate the effects of alcohol stimulation during adolescence on learning and memory and synaptic vesicles expression in hippocampus of adult rats. Methods: forty puberty SD rats were divided into two groups: control group and experimental group. The animals in the two groups were injected with saline 2g / kg alcohol for 5 days, fixed twice a day, then fed normally. The spatial discriminant learning and memory were tested by Morris water maze after adulthood. The behavior of rats was photographed by video camera and the results of water maze detection were analyzed by statistical analysis software. The expression of synaptophysin in hippocampal structure of normal control group and experimental group was detected by immunohistochemical method. Results: the time of escaping latency (EL) of rats in each group was shortened with the increase of training times. The escape latency (EL) of alcohol group was longer than that of normal control group (P < 0. 05). Immunoreactivity of synaptophysin: the immunoreactivity of synaptophysin was mainly expressed in CA_3 and dentate gyrus of hippocampal structure. Compared with alcohol group, the average gray value of CA_3 and synaptic vesicle immunoreactive products in rat hippocampal structure was significantly different from that in alcohol group (P < 0. 05). Conclusion: the expression of synaptic vesicles in CA_3, dentate gyrus of adult rats exposed to alcohol in adolescence decreased the escape latency prolonged and the ability of spatial learning and memory decreased in Morris water maze test.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R363

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