【摘要】：目的:通過建立一理想的動物模型來模擬T細胞急性淋巴細胞白血病的發(fā)病狀態(tài),為探索急性淋巴細胞白血病全新的治療方法提供平臺。方法:采用抗鼠-CD122抗體注射NOD/SCID小鼠進行預(yù)處理,通過尾靜脈注射9例不同病例的白血病細胞,以及1株T-ALL細胞系。通過檢測小鼠體內(nèi)白血病細胞及臟器白血病細胞浸潤情況,觀察白血病細胞植入。將白血病細胞進行二次移植,觀察移植穩(wěn)定性。對白血病動物模型進行藥物處理,觀察小鼠生存期,模擬人體治療反應(yīng)。結(jié)果:有4例病例的細胞及T-ALL細胞株成功植入。流式細胞檢測顯示受體小鼠體內(nèi)較多比例人CD45+細胞表達,免疫組化顯示CD45+細胞浸潤小鼠不同臟器,系列移植也獲得成功。體內(nèi)藥物處理顯示地塞米松能延長小鼠的生存期,與臨床觀察相一致。結(jié)論:成功建立經(jīng)抗鼠CD122單抗預(yù)處理的人T細胞急性淋巴細胞白血病NOD/SCID小鼠模型,具有原發(fā)疾病的所有主要特征。
[Abstract]:Objective: to establish an ideal animal model to simulate the pathogenesis of T cell acute lymphoblastic leukemia (AML) and to provide a platform for exploring a new treatment for acute lymphoblastic leukemia (ALL). Methods: NOD/SCID mice were pretreated with anti-mouse CD122 antibody, 9 leukemia cells from different cases and 1 T-ALL cell line were injected via tail vein. The infiltration of leukemic cells and visceral leukemic cells in mice was detected and the implantation of leukemic cells was observed. Leukemia cells were transplanted twice and the stability of transplantation was observed. The animal model of leukemia was treated with drugs, the survival time of mice was observed, and the response of human body was simulated. Results: cells and T-ALL cell lines were successfully implanted in 4 cases. Flow cytometry showed that more proportion of human CD45 cells were expressed in recipient mice and CD45 cells infiltrated different organs of mice by immunohistochemistry. Series transplantation was also successful. Drug treatment in vivo showed that dexamethasone could prolong the survival time of mice, which was consistent with clinical observation. Conclusion: human T cell acute lymphoblastic leukemia (NOD/SCID) mouse model pretreated with anti mouse CD122 monoclonal antibody has all the main characteristics of primary disease.
【作者單位】： 華中科技大學(xué)同濟醫(yī)學(xué)院附屬同濟醫(yī)院血液科;南京中醫(yī)藥大學(xué)附屬江蘇省中醫(yī)院血液科;
【基金】：華中科技大學(xué)博士點基金(200804870008)
【分類號】：R-332;R733.71

【參考文獻】

相關(guān)期刊論文 前2條

1 許思苗;楊漾;周密;趙雪嬌;秦宇;張沛凌;原瑞鳳;周劍峰;方勇;;逆轉(zhuǎn)錄病毒介導(dǎo)的小鼠MLL-AF9白血病模型的建立[J];中國實驗血液學(xué)雜志;2013年05期

2 孔圓;劉艷榮;劉開彥;黃曉軍;Fumihiko Ishikawa;Mine Harada;;人急性B淋巴細胞白血病-NOD/SCID/IL2rγ~(null)新生小鼠異種移植模型的建立[J];中國實驗血液學(xué)雜志;2012年03期

【共引文獻】

相關(guān)期刊論文 前8條

1 王迪;王娜;張艷;馬淑燕;耿哲;周鵬飛;周劍峰;黃亮;;Establishment of Reproducible Xenotransplantation Model of T Cell Acute Lymphoblastic Leukemia in NOD/SCID Mice[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2012年04期

2 商臻;王玨;王迪;肖敏;李童娟;王娜;黃亮;周劍峰;;Establishment of Xenotransplantation Model of Human CN-AML with FLT3-ITD~(mut)/NPM1 in NOD/SCID Mice[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2013年03期

3 賴穎暉;賴永榕;盧玉英;莫武寧;;人胎兒血造血干/祖細胞的生物學(xué)特性及其移植實驗(英文)[J];中國臨床康復(fù);2006年29期

4 胡東,黃士昂;人造血干細胞的免疫缺陷小鼠移植模型研究與應(yīng)用[J];中國實驗血液學(xué)雜志;2004年06期

5 孫婉玲;韓冰;汪玄;鐘玉萍;莊俊玲;沈悌;武永吉;孫婉玲;;陣發(fā)性睡眠性血紅蛋白尿癥CD34~+CD59~+細胞長期造血的動物實驗[J];中華醫(yī)學(xué)雜志;2007年42期

6 孔圓;王亞哲;胡s，

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