【摘要】：目的:通過建立一理想的動物模型來模擬T細(xì)胞急性淋巴細(xì)胞白血病的發(fā)病狀態(tài),為探索急性淋巴細(xì)胞白血病全新的治療方法提供平臺。方法:采用抗鼠-CD122抗體注射NOD/SCID小鼠進(jìn)行預(yù)處理,通過尾靜脈注射9例不同病例的白血病細(xì)胞,以及1株T-ALL細(xì)胞系。通過檢測小鼠體內(nèi)白血病細(xì)胞及臟器白血病細(xì)胞浸潤情況,觀察白血病細(xì)胞植入。將白血病細(xì)胞進(jìn)行二次移植,觀察移植穩(wěn)定性。對白血病動物模型進(jìn)行藥物處理,觀察小鼠生存期,模擬人體治療反應(yīng)。結(jié)果:有4例病例的細(xì)胞及T-ALL細(xì)胞株成功植入。流式細(xì)胞檢測顯示受體小鼠體內(nèi)較多比例人CD45+細(xì)胞表達(dá),免疫組化顯示CD45+細(xì)胞浸潤小鼠不同臟器,系列移植也獲得成功。體內(nèi)藥物處理顯示地塞米松能延長小鼠的生存期,與臨床觀察相一致。結(jié)論:成功建立經(jīng)抗鼠CD122單抗預(yù)處理的人T細(xì)胞急性淋巴細(xì)胞白血病NOD/SCID小鼠模型,具有原發(fā)疾病的所有主要特征。
[Abstract]:Objective: to establish an ideal animal model to simulate the pathogenesis of T cell acute lymphoblastic leukemia (AML) and to provide a platform for exploring a new treatment for acute lymphoblastic leukemia (ALL). Methods: NOD/SCID mice were pretreated with anti-mouse CD122 antibody, 9 leukemia cells from different cases and 1 T-ALL cell line were injected via tail vein. The infiltration of leukemic cells and visceral leukemic cells in mice was detected and the implantation of leukemic cells was observed. Leukemia cells were transplanted twice and the stability of transplantation was observed. The animal model of leukemia was treated with drugs, the survival time of mice was observed, and the response of human body was simulated. Results: cells and T-ALL cell lines were successfully implanted in 4 cases. Flow cytometry showed that more proportion of human CD45 cells were expressed in recipient mice and CD45 cells infiltrated different organs of mice by immunohistochemistry. Series transplantation was also successful. Drug treatment in vivo showed that dexamethasone could prolong the survival time of mice, which was consistent with clinical observation. Conclusion: human T cell acute lymphoblastic leukemia (NOD/SCID) mouse model pretreated with anti mouse CD122 monoclonal antibody has all the main characteristics of primary disease.
【作者單位】： 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬同濟(jì)醫(yī)院血液科;南京中醫(yī)藥大學(xué)附屬江蘇省中醫(yī)院血液科;
【基金】：華中科技大學(xué)博士點(diǎn)基金(200804870008)
【分類號】：R-332;R733.71

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