【摘要】：目的探討葡聚糖硫酸鈉(DSS)對BALB/C小鼠肝損傷及其纖維化的影響。方法 50只BALB/C小鼠隨機分成正常組、對照組和觀察組(6、12周),每組10只,對照組小鼠用5%四氯化碳(CCl4)橄欖油溶液誘導肝纖維化模型,觀察組小鼠用3.5%DSS灌胃誘導肝損傷及纖維化模型。HE染色觀察肝臟病理損傷,Massion染色觀察肝臟纖維化情況,改良賴氏法檢測血清谷丙轉氨酶(ALT)和谷草轉氨酶(AST)活性,ELISA法檢測血清Ⅰ型前膠原羧基端原肽(PⅠCP)和Ⅲ型前膠原肽(PⅢNP)含量,免疫組化檢測肝臟α-平滑肌肌動蛋白(α-SMA)表達變化。結果對照組和觀察組小鼠各時間段肝臟的病理損傷明顯,膠原纖維表達量增多,血清中ALT和AST活性增強、PⅠNP和PⅢNP含量增加,肝內α-SMA表達量增加,上述變化以對照組最明顯,觀察組12周次之,觀察組與對照組比較,差異有統(tǒng)計學意義(P0.05)。結論 DSS能有效誘導BALB/C小鼠肝纖維化,其誘導機制值得深入研究。
[Abstract]:Objective to investigate the effects of sodium dextran sulfate (DSS) on liver injury and fibrosis in BALB/C mice. Methods Fifty BALB/C mice were randomly divided into control group (n = 10), control group (n = 10) and observation group (n = 10). The control group was treated with 5% CCl4 olive oil solution to induce hepatic fibrosis. The liver injury and fibrosis model induced by 3.5%DSS were used in the observation group. The pathological injury of liver was observed by HE staining. The activities of serum alanine aminotransferase (ALT) and glutamic oxalacetic transaminase (AST) were detected by modified Lai's method. The levels of type 鈪，

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