【摘要】： 目的:通過(guò)比較豚鼠、SD大鼠兩種動(dòng)物高脂血癥模型的特點(diǎn)及兩種動(dòng)物高脂血清對(duì)HUVEC損傷的影響,為治療和預(yù)防高脂血癥的研究選擇理想的動(dòng)物模型提供試驗(yàn)依據(jù)。方法:將豚鼠、SD大鼠各20只分別隨機(jī)分為對(duì)照組和模型組。對(duì)照組用基礎(chǔ)飼料飼養(yǎng),模型組用高脂飼料飼養(yǎng)。各組均于連續(xù)飼養(yǎng)30d后采血,測(cè)定各項(xiàng)血脂指標(biāo)。取肝臟、主動(dòng)脈進(jìn)行病理學(xué)檢查。收集各組血清,并以此血清分組培養(yǎng)人臍靜脈血管內(nèi)皮細(xì)胞(HUVEC),用酶聯(lián)免疫法(ELISA)檢測(cè)培養(yǎng)上清液的血管性假血友病因子(vWF)。結(jié)果:30d造模期后,豚鼠高脂血癥模型組與SD大鼠高脂血癥模型組相比:1、豚鼠的體重增長(zhǎng)變化更為顯著(P＜0.01)。2、豚鼠TC、TG、LDL-C升高程度明顯高于SD大鼠(P＜0.01)。3、豚鼠肝臟脂肪變程度較SD大鼠重。4、豚鼠轉(zhuǎn)氨酶(ALT、AST)明顯升高(P＜0.01),而SD大鼠無(wú)變化。5、豚鼠肝組織SOD活性降低較SD大鼠明顯(P＜0.05)。6、豚鼠的動(dòng)脈硬化指數(shù)(AI)升高明顯,其主動(dòng)脈內(nèi)膜能形成動(dòng)脈粥樣硬化。7、豚鼠高脂血清對(duì)HUEVC的損傷程度更重;豚鼠上清液vWF含量升高明顯,且48h較24h升高更為顯著(P＜0.01)。結(jié)論:豚鼠較具有抗AS形成能力的大鼠而言,更適用于高脂血癥、AS等的研究。
[Abstract]:Objective: to compare the characteristics of two animal hyperlipidemia models in guinea pig Sprague-Dawley (SD) rats and the effects of two kinds of animal hyperlipidemic serum on HUVEC injury, so as to provide experimental basis for selecting ideal animal models for the treatment and prevention of hyperlipidemia. Methods: 20 guinea pig SD rats were randomly divided into control group and model group. The control group was fed with basic diet, while the model group was fed with high fat diet. Blood samples were collected after 30 days of continuous feeding, and blood lipid indexes were measured. The liver and aorta were taken for pathological examination. Human umbilical vein endothelial cells (HUVEC) were cultured in each group. The levels of von Willebrand factor (vWF) in the supernatant were detected by Elisa. Results after 30 days of modeling, Compared with SD rat hyperlipidemia model group, the weight gain of guinea pigs in the guinea pig hyperlipidemia model group was more significant (P < 0.01) .2.The increase of LDL-C in guinea pigs was significantly higher than that in SD rats (P < 0.01). 3. The fatty degree of guinea pig liver was heavier than that of SD rats. (4) the activity of SOD in guinea pig liver tissue was significantly lower than that in SD rat (P < 0.05), and the arteriosclerosis index (AI) of guinea pig was significantly higher than that of SD rat (P < 0.01), while the activity of SOD in guinea pig liver was significantly lower than that in SD rat (P < 0.01), while the activity of SOD in guinea pig liver was lower than that in SD rat (P < 0.05). The intima of aorta could form atherosclerosis. 7, the damage degree of guinea-pig hyperlipidemia serum to HUEVC was more serious, and the content of vWF in guinea-pig supernatant was significantly higher than that in 24h (P < 0.01). Conclusion: the guinea pig is more suitable for the study of hyperlipidemia and as than the rats with anti-atherogenic ability.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R589.2;R-332

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