本文選題：脊柱側(cè)彎 + 雙足大鼠��； 參考：《復(fù)旦大學(xué)》2009年碩士論文

【摘要】： 目的 青少年特發(fā)性脊柱側(cè)彎(adolescent idiopathic scoliosis,AIS)是一種嚴(yán)重危害青少年健康的疾病,AIS已經(jīng)是青少年繼視力異常、肥胖、包莖和社會(huì)心理障礙后的第五大常見(jiàn)病。它的病因至今未明,其中病因研究的熱點(diǎn)集中到神經(jīng)內(nèi)分泌系統(tǒng),而褪黑素與鈣調(diào)蛋白的研究更是炙手可熱,已有研究表明褪黑素減少是其中一個(gè)重要病因,鈣調(diào)蛋白與脊柱側(cè)彎的嚴(yán)重性成正比。動(dòng)物模型是病因?qū)W研究的基礎(chǔ)。很多研究通過(guò)已知的病因制作了許多動(dòng)物模型,但是至今仍未找到制作理想脊柱側(cè)彎動(dòng)物模型的辦法。本實(shí)驗(yàn)希望通過(guò)向雙足大鼠腹腔注射褪黑素拮抗劑制作特發(fā)性脊柱側(cè)彎模型,從而探討褪黑素、鈣調(diào)蛋白與特發(fā)性脊柱側(cè)彎的關(guān)系,為進(jìn)一步研究其病因奠定一定的實(shí)驗(yàn)基礎(chǔ)。 方法 健康的剛斷乳的SD大鼠30只,適應(yīng)性生長(zhǎng)一周,隨機(jī)分為5組,每組為6只。ABCD四組為雙足大鼠組,截去雙前肢和尾巴。ABC三組每天晚上6點(diǎn)到8點(diǎn),腹腔注射褪黑素拮抗劑luzindole,劑量分別為0.2mg/kg、0.5mg/kg、1mg/kg,使用生理鹽水的體積均為10ml。D組每天相同時(shí)間腹腔注射相同體積的生理鹽水。E組為四足大鼠組,作為空白對(duì)照。處死前,抽各組的靜脈血,離心分離出血小板,用ELISA法測(cè)定各組血小板中的鈣調(diào)蛋白水平。 結(jié)果 1.ABC三組均發(fā)生了脊柱側(cè)彎,發(fā)生率為38.9%,其中A組發(fā)生的脊柱側(cè)彎顯著,DE兩組均未發(fā)生脊柱側(cè)彎,未發(fā)生率為100%。 2.五組平均體重最重的是注射褪黑素拮抗劑劑量最大的C組。 3.各組血小板中的鈣調(diào)蛋白水平未見(jiàn)明顯的差異(p＞0.05)。 結(jié)論 1.本實(shí)驗(yàn)可成功建立雙足大鼠脊柱側(cè)彎模型,重復(fù)性好,并證實(shí)了脊柱側(cè)彎的發(fā)生與褪黑素的減少有關(guān)。使用褪黑素拮抗劑luzindole的劑量0.2mg/kg能成功建立脊柱側(cè)彎模型。 2.褪黑素拮抗劑luzindole可促進(jìn)大鼠的生長(zhǎng)。 3.血小板中的鈣調(diào)蛋白水平與特發(fā)性脊柱側(cè)彎未見(jiàn)明顯的相關(guān)性。
[Abstract]:Objective: adolescent idiopathic scoliosis (adolescent idiopathic) is the fifth most common disease in adolescents after abnormal vision, obesity, phimosis and psychosocial disorders. The etiology of melatonin is still unknown, among which the focus of etiological research is focused on the neuroendocrine system, and the studies of melatonin and calmodulin are even hotter. It has been shown that the decrease of melatonin is one of the important causes. Calmodulin is proportional to the severity of scoliosis. Animal models are the basis of etiological research. Many studies have produced many animal models based on known etiology, but no method has been found to make ideal animal models of scoliosis. The purpose of this study was to investigate the relationship between melatonin, calmodulin and idiopathic scoliosis by intraperitoneal injection of melatonin antagonist in bipedal rats, so as to lay a foundation for further study on the etiology of idiopathic scoliosis. Methods Thirty healthy weaned SD rats were randomly divided into 5 groups: 6 rats in each group. ABCD group 4 was biped rats. The forelimbs and tail were amputated from 6 pm to 8 o'clock every night in the three groups. The dose of melatonin antagonist luzindolewas 0.2 mg / kg ~ 0.5 mg / kg ~ (-1 mg / kg), and the volume of normal saline was 10 ml 路d ~ (-1) mg 路kg ~ (-1) 路g ~ (-1) 路kg ~ (-1) respectively. Before death, platelets were isolated by centrifugation, and calmodulin levels in platelets were measured by Elisa. Results 1. The incidence of scoliosis was 38.9 in ABC group, and there was no scoliosis in group A (100.2%). The heaviest average weight of the five groups was group C, which received the largest dose of melatonin antagonist. There was no significant difference in calmodulin levels in platelets of each group (p > 0.05). Conclusion 1. The results showed that the occurrence of scoliosis was related to the decrease of melatonin. The model of scoliosis was successfully established by using the dose 0.2mg/kg of melatonin antagonist luzindole. 2. Melatonin antagonist luzindole can promote the growth of rats. There was no significant correlation between calmodulin levels in platelets and idiopathic scoliosis.
【學(xué)位授予單位】：復(fù)旦大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類(lèi)號(hào)】：R682;R-332

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