本文選題：動脈結(jié)扎 + 自身免疫性睪丸炎��； 參考：《新疆醫(yī)科大學(xué)》2009年碩士論文

【摘要】： 目的:本實(shí)驗(yàn)通過結(jié)扎小鼠一側(cè)睪丸動脈并于不同時間后再通以誘導(dǎo)自身免疫性睪丸炎,觀察睪丸生精上皮、血液中抗精子抗體(AsAb)IgG、IgM以及遲發(fā)型超敏反應(yīng)的變化。實(shí)驗(yàn)探討了一側(cè)睪丸動脈結(jié)扎再通后,小鼠發(fā)生自身免疫性睪丸炎的機(jī)制,并試圖將此作為一種新型的構(gòu)建小鼠自身免疫性睪丸炎模型的方法。方法:選用健康成年雄性BALB/c小鼠,隨機(jī)分為4組:對照組(A)(麻醉后僅進(jìn)行陰囊切開后縫合),實(shí)驗(yàn)1組(S1)(單側(cè)睪丸動脈結(jié)扎,2小時后再開通血管),實(shí)驗(yàn)2組(S2)(單側(cè)睪丸動脈結(jié)扎,4小時后開通血管),實(shí)驗(yàn)3組(S3)(單側(cè)睪丸動脈結(jié)扎,6小時后再開通血管)。分別于術(shù)后12h,2周,4周應(yīng)用光鏡觀察石蠟包埋HE染色后的小鼠睪丸組織結(jié)構(gòu),應(yīng)用透射電鏡技術(shù)觀察術(shù)后12小時小鼠睪丸組織的超微結(jié)構(gòu)。采用ELISA法檢測2周、4周時各組小鼠血液中的抗精子抗體(AsAb)IgG、IgM濃度水平;采用游標(biāo)卡尺測量單側(cè)動脈結(jié)扎再通后對睪丸自身抗原的足墊反應(yīng)。結(jié)果:1.HE染色觀察:①術(shù)后12h的,A組小鼠睪丸生精小管內(nèi)無生精細(xì)胞脫落,各級生精細(xì)胞排列整齊、層次清楚,結(jié)構(gòu)正常,顯示活躍的精子發(fā)生。間質(zhì)中可見間質(zhì)細(xì)胞正常,核大而圓,偏位。S1組小鼠術(shù)側(cè)睪丸生精小管較為完整,小管內(nèi)可見輕微的生精細(xì)胞脫落,偶見生精小管內(nèi)空泡變。S3組小鼠術(shù)側(cè)睪丸生精小管之間間隙增寬,生精小管內(nèi)生精細(xì)胞層數(shù)減少。間質(zhì)中血管充血明顯,管腔中充滿了紅細(xì)胞,血管壁增厚呈玻璃樣變,同時血管內(nèi)近壁側(cè)可以看見較多的炎性細(xì)胞(主要是白細(xì)胞)的浸潤。S4組小鼠術(shù)側(cè)睪丸間質(zhì)中可以看到較多的炎性細(xì)胞浸潤。睪丸生精小管內(nèi)生精細(xì)胞層數(shù)減少,看不到精子,細(xì)胞腫脹明顯,胞質(zhì)透亮。可以看到多核巨細(xì)胞散落分布在睪丸生精小管管腔中。各組非術(shù)側(cè)睪丸生精上皮規(guī)則,生精細(xì)胞排列有序,可見大量精子。間質(zhì)中可見間質(zhì)細(xì)胞,核大而圓,偏位。②術(shù)后2周的光鏡結(jié)果:A組小鼠睪丸組織結(jié)構(gòu)正常,與術(shù)后12h無顯著差異。S1組睪丸生精小管內(nèi)生精細(xì)胞層數(shù)減少,可見空泡狀結(jié)構(gòu)。S2、S3組均可見不同程度的生精細(xì)胞脫落、排列紊亂,生精上皮變薄、空泡樣變。間質(zhì)中血管增多、血管壁變厚,在血管周圍和生精小管界膜附近,可見炎性細(xì)胞浸潤。③術(shù)后4周時各組光鏡結(jié)果與2周時相比較,無明顯結(jié)構(gòu)上的差異。2.透射電鏡觀察:A組小鼠睪丸生精上皮結(jié)構(gòu)完整,細(xì)胞無腫脹變性;其余各手術(shù)組均可見不同程度的睪丸生精細(xì)胞腫脹變性壞死。3.血清抗精子抗體IgG與IgM檢測結(jié)果:各實(shí)驗(yàn)組與正常對照組比較,血清中抗精子抗體IgG及IgM的濃度明顯升高,具有統(tǒng)計(jì)學(xué)意義(P0.05);4.足趾腫脹實(shí)驗(yàn):各手術(shù)組均出現(xiàn)遲發(fā)型過敏反應(yīng)。結(jié)論:單側(cè)睪丸動脈結(jié)扎/再通后:1.術(shù)側(cè)睪丸組織首先表現(xiàn)為一種缺血性的炎癥表現(xiàn),有大量的炎性細(xì)胞的浸潤(主要是白細(xì)胞);2.術(shù)后雖然恢復(fù)了血液供應(yīng),但仍可見小鼠睪丸生精上皮脫落明顯,生精細(xì)胞層數(shù)減少,可見炎細(xì)胞浸潤(主要為單個核細(xì)胞)。3.血清中有抗精子抗體IgG、IgM出現(xiàn)。4.可以刺激機(jī)體形成遲發(fā)型超敏反應(yīng)。5.此種手術(shù)方法可以成功建立自身免疫性睪丸炎模型。
[Abstract]:Objective: to induce autoimmune orchitis by ligating one of the testicular arteries in mice at a different time and observing the changes in the spermatogenic epithelium of the testis and the changes of anti sperm antibody (AsAb) IgG, IgM and delayed hypersensitivity in the blood. Mechanism, and try to use this as a new model of mouse autoimmune orchitis. Methods: healthy adult male BALB/c mice were randomly divided into 4 groups: control group (A) (after anaesthesia only after scrotal incision suture), experimental 1 (S1) (unilateral testicular artery ligation, 2 hours after the reopening of blood vessels), experimental 2 (S2) (single The lateral testis artery was ligated and the blood vessels were opened 4 hours later. The experimental 3 groups (S3) (unilateral testicular artery ligation and reopening of blood vessels after 6 hours) were used to observe the histological structure of the testis after paraffin embedded HE staining in 12h, 2 weeks and 4 weeks after the operation, and the ultrastructure of the testis in 12 hours after the operation was observed by transmission electron microscopy. ELISA was used to observe the ultrastructure of the testis in the mice. The method was used to detect the level of antisperm antibody (AsAb) IgG and IgM in the blood of each group for 2 weeks and 4 weeks. The foot pad reaction of the autoantigen of the testis was measured by the vernier caliper and after the unilateral artery was ligated and repassed. Results: 1.HE staining was used to observe: (1) after 12h, the no spermatogenic cells in the testicular seminiferous tubules of the A mice were lost, and the spermatogenic cells at all levels were arranged neatly. The interstitial cells were normal, the interstitial cells were normal, the nucleus was large and round, and the testicular seminiferous tubules on the side of the.S1 group were more complete, and the minor spermatogenic cells in the tubule were seen in the tubule. The interstitial cells in the vacuoled.S3 group of the spermatogenic tubule group were widened in the space between the seminiferous tubules and the seminiferous tubules. The number of spermatogenic cells decreased. The vascular congestion was obvious in the interstitium, red blood cells were filled in the lumen, the thickening of the wall of the blood vessel was glass like change. At the same time, more inflammatory cells (mainly leucocytes) in the proximal wall of the blood vessels were seen in the.S4 group. The number of cell layers was reduced, the sperm was not visible, the cells were swollen and the cytoplasm was bright. It could be seen that the multinuclear giant cells scattered in the seminiferous tubules. The rules of the spermatogenic epithelium in the non lateral testis were regular, the spermatogenic cells were arranged in order, and a large number of spermatozoa were visible. The interstitial cells were visible in the interstitium, and the nucleus was large and round and partial. (2) the results of light microscopy 2 weeks after the operation: A The histological structure of the testis was normal in the group of mice. There was no significant difference between the 12h and the postoperatively. The number of spermatogenic cells in the testicular seminiferous tubules in the.S1 group decreased, and the vacuolated structure was.S2. The S3 group showed the exfoliation of spermatogenic cells in different degrees, the disordered arrangement, the thinning of the spermatogenic epithelium and the vacuolation. The blood Guan Zengduo, the blood vessel wall thickened, and the peripheral blood vessels and the seminiferous tubules In the 4 weeks after the operation, the results of the light microscopy were compared with the 2 weeks, and there was no obvious difference in structure.2. transmission electron microscopy: the spermatogenic epithelium of the mice in group A was complete and the cells were not swollen and denatured, and the other surgical groups showed a different degree of testicular spermatocyte swelling and necrosis of.3. serum ANTISPERMATOGENESIS. The results of the detection of subantibody IgG and IgM: compared with the normal control group, the concentration of antisperm antibody IgG and IgM in the serum was significantly increased, with statistical significance (P0.05); 4. toe swelling test: all the operation groups had delayed anaphylaxis. Conclusion: the unilateral testicular vein ligation / repassage: the 1. operative side of the testis is the first manifestation of one kind. There was a large number of inflammatory cells infiltrating (mainly white blood cells). After 2., the blood supply was restored, but the spermatogenic epithelium of mouse testis was obvious, the number of spermatogenic cells decreased, and the infiltration of inflammatory cells (mainly mononuclear cells) had anti sperm antibody IgG in.3. serum, and.4. could be stimulated by IgM. .5. is a delayed type hypersensitivity reaction. This method can successfully establish the autoimmune orchitis model.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R-332;R697.22

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 聶振英,吳俊,劉斌;睪丸炎動物模型的建立(一)——對睪丸形態(tài)結(jié)構(gòu)的研究[J];北京醫(yī)科大學(xué)學(xué)報(bào);1998年04期

2 王滿貴,宋志宙;HE染色切片的技術(shù)操作規(guī)范[J];大同醫(yī)學(xué)專科學(xué)校學(xué)報(bào);2004年02期

3 陳平;蘇東輝;蘭風(fēng)華;;抗精子抗體與免疫不育的研究進(jìn)展[J];福建醫(yī)科大學(xué)學(xué)報(bào);2006年01期

4 鄭旭琴,孫敏;催乳素與人類腫瘤關(guān)系的新認(rèn)識[J];國外醫(yī)學(xué).內(nèi)分泌學(xué)分冊;2004年02期

5 余楠;環(huán)境雌激素與人類疾病[J];國外醫(yī)學(xué).臨床生物化學(xué)與檢驗(yàn)學(xué)分冊;2002年02期

6 賈頤舫,吳愛華,張愛東;Y染色體AZF基因缺失與原發(fā)性無精子癥的相關(guān)性研究[J];國外醫(yī)學(xué).遺傳學(xué)分冊;2005年01期

7 林震;高泌乳素血癥與性和生育[J];國外醫(yī)學(xué).泌尿系統(tǒng)分冊;1996年04期

8 青成言;無精子癥生殖激素測定18例臨床分析[J];貴州醫(yī)藥;2002年08期

9 邱東茹,吳振斌,賀鋒;內(nèi)分泌擾亂化學(xué)品對動物的影響和作用機(jī)制[J];環(huán)境科學(xué)研究;2000年06期

10 向桂橋,陳榮安,張益康,江利,許良余;6498例男性不育癥病因分析[J];南華大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2005年02期

相關(guān)碩士學(xué)位論文 前1條

1 宋非無;隱睪癥中雌激素水平和雌激素受體亞型-α、β的研究[D];福建醫(yī)科大學(xué);2007年

，

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