本文選題：骨髓間充質(zhì)干細(xì)胞 + 神經(jīng)生長相關(guān)蛋白-43��； 參考：《吉林大學(xué)》2008年碩士論文

【摘要】： 目的: 觀察β-巰基乙醇及腦源性神經(jīng)營養(yǎng)因子(brain derived neurotrophic factor,BDNF)誘導(dǎo)下大乳鼠骨髓間充質(zhì)干細(xì)胞向神經(jīng)樣細(xì)胞分化后神經(jīng)生長相關(guān)蛋白-43(growth associated protein- 43,GAP-43)的表達(dá)變化,以探討其在骨髓間充質(zhì)干細(xì)胞定向分化過程中的作用。 方法: 體外培養(yǎng)大鼠骨髓間充質(zhì)干細(xì)胞,經(jīng)流式細(xì)胞儀檢測細(xì)胞表面標(biāo)志物進(jìn)行鑒定,分別以β-巰基乙醇及腦源性神經(jīng)營養(yǎng)因子誘導(dǎo)其向神經(jīng)元樣細(xì)胞方向分化,觀察誘導(dǎo)后細(xì)胞形態(tài)學(xué)變化,應(yīng)用免疫細(xì)胞化學(xué)技術(shù)對分化后細(xì)胞進(jìn)行鑒定,分析神經(jīng)巢蛋白(nestin)、神經(jīng)元特異性烯醇化酶(NSE)、神經(jīng)絲蛋白(NF)的表達(dá)情況,并觀察分化后的GAP-43的表達(dá)變化。 結(jié)果: 體外培養(yǎng)所得BMSCs經(jīng)流式細(xì)胞儀檢測CD90(+)、CD71(+)、CD29(+),CD44(-),經(jīng)兩種方法誘導(dǎo)BMSCs均可分化為神經(jīng)樣細(xì)胞,分化后細(xì)胞均表達(dá)GAP-43、NSE、NF。誘導(dǎo)后1,2,4,6,8小時(shí)GAP-43表達(dá)陽性率兩組均逐漸升高,GAP-43表達(dá)強(qiáng)度兩組均在誘導(dǎo)后8h內(nèi)先增強(qiáng)后下降,以誘導(dǎo)后4h表達(dá)達(dá)到高峰。 結(jié)論: 1、骨髓間充質(zhì)干細(xì)胞可以在體外分離及培養(yǎng)。 2、骨髓間充質(zhì)干細(xì)胞可以在體外誘導(dǎo)分化為類神經(jīng)樣細(xì)胞。 3、GAP-43在骨髓間充質(zhì)干細(xì)胞向類神經(jīng)樣細(xì)胞分化過程中可能起到某種作用甚至是支配作用。
[Abstract]:Aim: to observe the expression of nerve growth related protein 43 (growth associated protein-43 (GAP-43) after differentiation of bone marrow mesenchymal stem cells into neural cells induced by 尾 -mercaptoethanol and brain-derived neurotrophic factor (brain derived neurotrophic). To investigate its role in the differentiation of bone marrow mesenchymal stem cells. Methods: rat bone marrow mesenchymal stem cells were cultured in vitro and identified by flow cytometry. 尾 -mercaptoethanol and brain-derived neurotrophic factor were used to induce the differentiation into neuron-like cells. The morphological changes after induction were observed, the differentiation cells were identified by immunocytochemistry, and the expression of neuron-specific enolase (NSE) and neurofilament protein (NF) of nestin (nestin), were analyzed. The expression of GAP-43 was observed after differentiation. Results: BMSCs cultured in vitro were detected for CD90 () CD71 () and CD29 () CD29 () CD44 (-) by flow cytometry. BMSCs were induced to differentiate into nerve like cells by two methods. The positive rate of GAP-43 expression in both groups increased gradually at 8 hours after induction. The expression intensity of GAP-43 in both groups increased first within 8 hours after induction and then decreased, and reached the peak at 4 hours after induction. Conclusion: 1. Bone marrow mesenchymal stem cells can be isolated and cultured in vitro. 2bone marrow mesenchymal stem cells can be induced to differentiate into neuron-like cells in vitro. Mesenchymal stem cells may play a certain role or even a dominant role in the process of differentiation into neuroblast-like cells.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R329

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