本文選題：重組疫苗 + 禽流感�。� 參考：《吉林大學(xué)》2009年碩士論文

【摘要】： 高致病性禽流感(HPAI)病毒,特別是H5N1毒株,可以在飼養(yǎng)和野生的鳥類種群有很高的發(fā)病率與死亡率,在最近的暴發(fā)流行導(dǎo)致了嚴(yán)重的經(jīng)濟(jì)損失。亞洲和歐洲相繼有H9N2、H7N7和H5N1亞型禽流感病毒感染人類的報(bào)道,而且疫情有日益嚴(yán)重的趨勢(shì)。2009年以來,我國(guó)數(shù)個(gè)省區(qū)陸續(xù)有人感染禽流感的報(bào)告。 HA(凝血素蛋白)不但在病毒分類及變異中是主要標(biāo)志,在抗病毒感染中也有很重要的作用,在現(xiàn)有的對(duì)禽流感病毒感染的免疫機(jī)制研究中認(rèn)為,HA蛋白是介導(dǎo)病毒進(jìn)入宿主細(xì)胞的重要配體分子,因此抗HA蛋白的中和抗體在阻斷HA介導(dǎo)病毒進(jìn)入細(xì)胞內(nèi)增殖的過程中具有重要的作用。HA是禽流感病毒的重要抗原成分,進(jìn)入機(jī)體后可引起特異性的免疫應(yīng)答,可避免再次接觸同種病毒時(shí)的感染。因此,誘導(dǎo)機(jī)體形成對(duì)HA蛋白的特異性免疫是預(yù)防禽流感的一個(gè)重要目標(biāo)。HA蛋白還可能是抗病毒藥物作用的靶點(diǎn)。 本研究的目的就是用原核系統(tǒng)重組H5蛋白,使其能夠模擬天然H5蛋白誘導(dǎo)有效的針對(duì)H5亞型禽流感病毒的免疫,在劉旭等人工作的基礎(chǔ)上,進(jìn)一步完善了重組H5蛋白L3的表達(dá)條件、純化方案,建立了較穩(wěn)定的間接ELISA實(shí)驗(yàn)體系檢測(cè)L3蛋白作為抗原檢測(cè)相應(yīng)抗體和滅活病毒作為抗原檢測(cè)相應(yīng)抗體,并且根據(jù)交叉反應(yīng)原理來評(píng)估重組蛋白L3與天然蛋白H5在免疫原性上的差異,初步評(píng)估重組蛋白是否可以作為新型禽流感疫苗應(yīng)用。
[Abstract]:The highly pathogenic avian influenza virus (HPAI), especially the H5N1 strain, can cause high morbidity and mortality in breeding and wild bird populations, resulting in serious economic losses in recent outbreaks. In Asia and Europe, human infection with H9N2FH7N7 and H5N1 subtype avian influenza virus has been reported, and the epidemic has become increasingly serious. In several provinces and regions of China, there have been reports of human infection with avian influenza. HAA (hemagglutinin protein) is not only the main marker of virus classification and mutation, but also plays an important role in anti-virus infection. In the current studies on the immune mechanism of avian influenza virus infection, it is considered that the HA protein is an important ligand that mediates virus entry into host cells. Therefore, the neutralizing antibody against HA protein plays an important role in blocking the proliferation of virus into cells mediated by HA. Ha is an important antigen component of avian influenza virus, and it can cause a specific immune response after entering the body. Can avoid the infection of contact with the same virus again. Therefore, inducing the formation of specific immunity to HA protein is an important target for the prevention of avian influenza. Ha protein may also be a target of antiviral drugs. The aim of this study is to recombine H5 protein with prokaryotic system, so that it can mimic the natural H5 protein to induce effective immunization against H5 subtype avian influenza virus, based on the work of Liu Xu et al. The expression conditions and purification schemes of recombinant H5 protein L3 were further improved. A stable indirect Elisa system was established to detect L3 protein as antigen and inactivated virus as antigen to detect corresponding antibody. The immunogenicity of recombinant protein L3 and natural protein H5 was evaluated according to the principle of cross reaction, and whether the recombinant protein could be used as a new avian influenza vaccine was preliminarily evaluated.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R392

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