發(fā)布時(shí)間：2018-06-18 01:48

  本文選題：寒燥環(huán)境 + 生物表征　； 參考：《新疆醫(yī)科大學(xué)》2009年碩士論文

【摘要】： 目的:研究西北干燥寒冷環(huán)境對小鼠生物表征的的影響,并揭示這種環(huán)境對機(jī)體產(chǎn)生影響的部分生物學(xué)基礎(chǔ),為西北寒燥證證候模型的建立及評價(jià)提供思路。方法:根據(jù)中醫(yī)病因?qū)W說理論,用人工氣候箱模擬新疆特有的干燥寒冷環(huán)境,并作用于ICR小鼠,觀察記錄小鼠各種生物表征的變化,在HE染色光鏡和透射電鏡下分別觀察小鼠各臟器組織形態(tài)和細(xì)胞超微結(jié)構(gòu)的改變,用高效液相色譜-電化學(xué)檢測法測定小鼠腦組織和外周血中單胺類神經(jīng)遞質(zhì)的變化。結(jié)果:和空白對照組相比,置于寒燥環(huán)境中的小鼠除出現(xiàn)一般寒、燥表現(xiàn)外,中期毛發(fā)稀疏少澤,逐漸變得煩躁、易怒,修飾反應(yīng)增加,對刺激反應(yīng)敏感,出現(xiàn)撕咬打斗現(xiàn)象;后期漸趨于安靜,對刺激表現(xiàn)較前遲鈍,毛色暗淡無光澤。所提取原色和亮度方面,以舌尖部的明顯變化出現(xiàn)最早,舌根部最晚;小鼠的爪、尾所提取原色和亮度數(shù)值的變化與舌的變化趨勢有著某種程度的一致性,而且爪與舌的相似度優(yōu)于尾與舌的相似度;前期兩組小鼠體重相比差異明顯,后期這種差異不具統(tǒng)計(jì)學(xué)意義;小鼠21天的進(jìn)食量和飲水量和空白對照組相比有明顯差異。寒燥環(huán)境組與空白對照組相比各種臟器在組織形態(tài)和超微結(jié)構(gòu)上有明顯改變。寒燥環(huán)境應(yīng)激21天小鼠血清中的去甲腎上腺素、多巴胺明顯低于對照組(P 0.01)五羥色胺高于對照組(P 0.05),而腦中去甲腎上腺素、多巴胺和5-羥色胺含量均明顯高于對照組(P 0.01)。結(jié)論:1、寒燥環(huán)境可引起小鼠各種生物表征的改變,且這種變化有著時(shí)程性;2、寒燥環(huán)境可能是西北寒燥證形成的主要原因;3、西北寒燥證不是某一臟器的改變,而是機(jī)體整體性、全身性、系統(tǒng)性的改變;4、寒燥證證候模型的建立,給沙漠周邊居民、石油工人、來疆民工及駐疆解放軍、武警指戰(zhàn)員等特殊人群的異常生物表征改變提供了部分生物學(xué)基礎(chǔ)研究的支撐,為下一步治療及預(yù)防工作的開展提供了實(shí)驗(yàn)支持;5、小鼠望診,可增加望爪和望尾兩部分。
[Abstract]:Objective: To study the effect of dry and cold environment on the biological characterization of mice in Northwest China, and to reveal the biological basis of the effect of this environment on the organism, and to provide ideas for the establishment and evaluation of the northwest cold dryness syndrome model. Method: according to the theory of TCM etiology, the special dry and cold environment of Xinjiang is simulated with a human working climate box. The changes of various biological characteristics of mice were observed and recorded in ICR mice. The changes of tissue morphology and ultrastructure of various organs of mice were observed under HE staining and transmission electron microscopy. The changes of monoamine neurotransmitters in the brain tissue and peripheral blood of mice were measured by high performance liquid chromatography electrochemical detection. Results: and blank control In the cold dry environment, the mice in the cold environment were not only cold and dry, but the middle hair was thinning and less, gradually became irritable, irritable, the reaction was increased, the reaction was sensitive to the stimulus, and the phenomenon of tearing and fighting appeared, and the later Yu Anjing was gradually dull and dull and dull. The obvious change of the tip was the earliest, the root of the tongue was the latest. The change of the original color and brightness values of the mice's claw and tail had some consistency with the change trend of the tongue, and the similarity between the tongue and the tongue was better than the similarity between the tail and the tongue; the two groups of mice in the early stage were obviously different in weight, and the difference was not statistically significant in the later period. Compared with the blank control group, the food intake and the amount of drinking water were significantly different between the 21 days and the blank control group. The cold dryness environment group and the blank control group had obvious changes in the tissue morphology and ultrastructure. The norepinephrine in the serum of 21 days in the cold dryness environment was significantly lower than the control group (P 0.01) and five HT was higher than the control group (P 0.05), the content of norepinephrine, dopamine and 5- hydroxytryptamine in the brain was significantly higher than that in the control group (P 0.01). Conclusion: 1, cold dryness environment can cause changes in various biological characterization of mice, and this change has time history; 2, cold dryness environment may be the main cause of the formation of northwest cold dryness syndrome; 3, northwest cold dryness syndrome is not a change of a certain organ, It is the integral, systemic and systematic change of the body; 4, the establishment of a cold dryness syndrome model provides support for the abnormal biological characterization of special populations around the desert surrounding residents, oil workers, migrant workers and the PLA, the armed police officers and other special groups of people, and for the next step of treatment and prevention. It provides experimental support. 5, the inspection of the mice can increase the two parts of the claw and the tail.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R256;R-332

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本文編號：2033458