本文選題：骨髓間充質(zhì)干細(xì)胞 + 細(xì)胞培養(yǎng)�。� 參考：《大連醫(yī)科大學(xué)》2009年碩士論文

【摘要】： 目的:研究小鼠骨髓間充質(zhì)干細(xì)胞(mesenchymal stem cells,MSCs)體外培養(yǎng)和擴(kuò)增及定向誘導(dǎo)分化為成骨細(xì)胞的可能性和方法。 方法:取C57BL小鼠雙側(cè)股骨和脛骨骨髓細(xì)胞進(jìn)行體外培養(yǎng),貼壁細(xì)胞傳代。取第5代細(xì)胞誘導(dǎo)培養(yǎng),倒置顯微鏡下觀察其形態(tài)。取誘導(dǎo)培養(yǎng)14天后的細(xì)胞進(jìn)行堿性磷酸酶染色和誘導(dǎo)培養(yǎng)21天后的細(xì)胞進(jìn)行vonkossa礦化結(jié)節(jié)染色。 結(jié)果:體外培養(yǎng)的MSCs生長(zhǎng)較好,原代和傳代細(xì)胞都具有很強(qiáng)的增殖能力,誘導(dǎo)培養(yǎng)后,ALP檢測(cè)和vonkossa礦化結(jié)節(jié)染色均呈陽(yáng)性反應(yīng)。 結(jié)論:MSCs能于體外培養(yǎng)存活和擴(kuò)增,并且在一定條件下可以誘導(dǎo)分化為成骨細(xì)胞,可用作骨組織工程的種子細(xì)胞。
[Abstract]:Aim: to study the possibility and method of osteoblast differentiation from mesenchymal stem cells (MSCs) derived from mouse bone marrow mesenchymal stem cells (MSCs) in vitro. Methods: bone marrow cells from bilateral femur and tibia of C57BL mice were cultured in vitro and the adherent cells were subcultured. The cells of the fifth passage were cultured and the morphology of the cells was observed under inverted microscope. The cells cultured for 14 days were stained with alkaline phosphatase and those cultured for 21 days were stained with vonkossa mineralized nodules. Results: MSCs grew well in vitro and both primary and passage cells had strong proliferative ability. After induction and culture, both ALP detection and vonkossa mineralized nodule staining showed positive reaction. Conclusion\
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 姜玉峰,李思源,慕曉玲;骨髓基質(zhì)細(xì)胞成骨的研究進(jìn)展[J];解剖學(xué)研究;2004年01期

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