本文選題：睡眠剝奪 + 空間學(xué)習(xí)記憶　； 參考：《復(fù)旦大學(xué)》2010年碩士論文

【摘要】：第一部分 目的觀察慢性不完全睡眠剝奪對青春期大鼠睡眠結(jié)構(gòu)的影響,探討睡眠剝奪后睡眠-覺醒周期有效恢復(fù)并保證睡眠結(jié)構(gòu)穩(wěn)態(tài)的最低睡眠時間。 方法未成年雄性SD大鼠隨機(jī)分為正常對照組(C)和4小時睡眠剝奪組(4hSD),予以輕觸等方法進(jìn)行人工不完全睡眠剝奪,連續(xù)7天。利用高度自動化睡眠覺醒解析平臺記錄分析大鼠睡眠的量、質(zhì)及睡眠時相構(gòu)成；同時觀察正常幼年大鼠在發(fā)育過程中睡眠結(jié)構(gòu)的變化。 結(jié)果1每天4h奪眠,連續(xù)7天后青春期大鼠睡眠量增加以黑天的NREM增加為主；SWA、Power density增加,以白天為主；此外覺醒、NREM睡眠和REM睡眠的之間轉(zhuǎn)換均降低,根據(jù)覺醒量明顯下降可以得出睡眠以長時間NREM為主。 2在奪眠后第1天睡眠結(jié)構(gòu)改變明顯,以奪眠后各睡眠-覺醒時相持續(xù)時間和各時相轉(zhuǎn)換變化明顯,奪眠后第7天以Power density變化明顯,而奪眠后第3天,奪眠后第5天睡眠反彈變化不明顯。 結(jié)論每天4h奪眠,連續(xù)7天可增加黑天的NREM睡眠量,增加白天的NREM和REM睡眠深度。 第二部分 目的觀察不同時間的慢性不完全睡眠剝奪對青春期大鼠以及成年大鼠的空間學(xué)習(xí)記憶能力及機(jī)制,探討不同時間的慢性不完全睡眠剝奪導(dǎo)致幼年和成年大鼠認(rèn)知功能障礙和海馬內(nèi)腺苷水平、PDE等分子表達(dá)之間聯(lián)系。 方法青春期和成年雄性SD大鼠隨機(jī)分為正常對照組(C)、2小時睡眠剝奪組(2h SD)、4小時睡眠剝奪組(4h SD)、6小時睡眠剝奪組(6h SD)以及8小時睡眠剝奪組(8h SD),連續(xù)奪眠7天。采用Morris水迷宮對所有大鼠進(jìn)行空間認(rèn)知功能訓(xùn)練,訓(xùn)練結(jié)束后予以輕觸等方法進(jìn)行人工不完全睡眠剝奪；利用Morris水迷宮的空間平臺定位實驗測定大鼠的空間記憶；運(yùn)用RT-PCR技術(shù)測定大鼠海馬腺苷受體以及PDE4的表達(dá)。 結(jié)果1、各組大鼠奪眠后學(xué)習(xí)訓(xùn)練(認(rèn)知功能)的潛伏期無明顯變化,4周齡(4w)大鼠奪眠2h即出現(xiàn)上臺前總路程增加,學(xué)習(xí)能力下降：2、4周齡大鼠奪眠6h后海馬腺苷受體A1R,A2AR水平增加,4周齡大鼠奪眠8h PDE4水平增加,而8周齡大鼠無明顯變化。 結(jié)論每天奪眠2h、4h、8h,連續(xù)奪眠7天,即降低青春期大鼠的水迷宮空間學(xué)習(xí)能力,而對成年大鼠的學(xué)習(xí)能力無變化,每天奪眠2h、8h,連續(xù)奪眠7天,降低了成年大鼠的水迷宮空間記憶能力,而對青春期大鼠的記憶能力無變化；實時定量RT-PCR結(jié)果顯示奪眠后7天,在奪眠6h組青春期大鼠A1,A2A受體升高,奪眠8h后青春期大鼠PDE4升高有明顯意義。
[Abstract]:Part one Objective to observe the effect of chronic incomplete sleep deprivation on sleep structure of puberty rats, and to explore the minimum sleep time of sleep structure homeostasis after sleep deprivation. Methods male SD rats were randomly divided into normal control group (C) and 4-hour sleep deprivation group (4h SDN). The rats were subjected to artificial incomplete sleep deprivation for 7 days. The sleep quantity, quality and sleep phase composition of the rats were analyzed by using the highly automated sleep awakening analysis platform, and the changes of sleep structure during the development of normal young rats were observed at the same time. Results 1the amount of sleep in puberty rats increased after 4 hours of sleep capture every day, the increase of NREM in dark day was the main increase of SWAEM-Power density, and the change of sleep between NREM and REM sleep was decreased in Awakening and Awakening (Awakening). According to the obvious decrease in arousal, it can be concluded that sleep is mainly NREM for a long time. 2Sleep structure changed obviously on the first day after sleep capture, and the duration of each sleep-wake phase and the change of each time phase were obvious after sleep capture, Power density changed obviously on the 7th day after sleep capture, and on the third day after sleep capture. The change of sleep rebound was not obvious on the 5th day after sleep capture. Conclusion 4 h daily sleep deprivation can increase the amount of NREM sleep and NREM and REM sleep depth in dark day for 7 days. Part two Objective to observe the spatial learning and memory ability and its mechanism of chronic incomplete sleep deprivation in adolescent and adult rats at different times. To investigate the relationship between chronic incomplete sleep deprivation and expression of adenosine in hippocampus and cognitive dysfunction in young and adult rats. Methods male Sprague-Dawley rats were randomly divided into 2 hour sleep deprivation group (control group) and 8 hour sleep deprivation group. The spatial cognitive function of all rats was trained by Morris water maze, and the artificial incomplete sleep deprivation was carried out by using light touch after training, and the spatial memory of the rats was measured by the Morris water maze spatial platform positioning experiment. RT-PCR technique was used to detect the expression of adenosine receptor and PDE4 in rat hippocampus. Results 1. There was no significant change in the latency of learning training (cognitive function) after sleep capture in each group. The level of A1R A2AR in hippocampus was increased in rats of 4 weeks old, but the level of PDE4 in rats of 8 weeks old did not change. Conclusion the spatial learning ability of water labyrinth in adolescent rats was reduced after 2 hours of sleep for 4 hours and 8 hours for 7 consecutive days. However, there was no change in learning ability of adult rats. The rats were deprived of sleep for 2 hours and 8 hours a day for 7 consecutive days. The spatial memory ability of water maze in adult rats was decreased, but the memory ability of puberty rats was not changed. The results of real-time quantitative RT-PCR showed that the A1A2A receptor of adolescent rats increased 7 days after sleep deprivation. The increase of PDE4 in pubertal rats after 8 h sleep capture has significant significance.
【學(xué)位授予單位】：復(fù)旦大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R338

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