本文選題：骨髓基質(zhì)干細(xì)胞 + 誘導(dǎo)分化�。� 參考：《蘇州大學(xué)》2009年碩士論文

【摘要】： 第一部分還原型谷胱甘肽誘導(dǎo)大鼠骨髓基質(zhì)干細(xì)胞分化過程中NSE、GFAP相關(guān)mRNA及蛋白表達(dá)變化研究 目的:初步探討還原型谷胱甘肽(GSH)對(duì)大鼠骨髓基質(zhì)干細(xì)胞(bone marrow stromalcells,BMSCs)的誘導(dǎo)分化機(jī)制。 方法:體外培養(yǎng)和純化大鼠BMSCs,用GSH對(duì)其進(jìn)行誘導(dǎo),分別于誘導(dǎo)后6h、12h、24h后對(duì)其進(jìn)行實(shí)時(shí)定量PCR和Westblot檢測(cè),測(cè)定各組中神經(jīng)元特異性烯醇化酶(neuron specific enolase,NSE)和膠質(zhì)纖維酸性蛋白(glial fibrillary acidic protein,GFAP)mRNA及蛋白水平的表達(dá)情況。 結(jié)果:誘導(dǎo)6h、12h、24h后BMSCs分化形成神經(jīng)元樣細(xì)胞和膠質(zhì)細(xì)胞樣細(xì)胞,實(shí)時(shí)定量PCR和Westblot檢測(cè)結(jié)果表明BMSCs中NSE及GFAP的mRNA與蛋白水平表達(dá)明顯高于對(duì)照組,以12h最為明顯。 結(jié)論:GSH可以誘導(dǎo)BMSCs分化形成神經(jīng)元樣細(xì)胞和膠質(zhì)細(xì)胞樣細(xì)胞,且誘導(dǎo)后NSE及GFAP的mRNA與蛋白水平表達(dá)增高。 第二部分堿性成纖維細(xì)胞生長(zhǎng)因子與表皮生長(zhǎng)因子誘導(dǎo)大鼠骨髓基質(zhì)干細(xì)胞向神經(jīng)元樣細(xì)胞分化相關(guān)基因表達(dá)譜研究 目的:初步探討大鼠骨髓基質(zhì)干細(xì)胞(BMSCs)向神經(jīng)元樣細(xì)胞分化前后基因表達(dá)譜的變化,尤其是與神經(jīng)元發(fā)育相關(guān)基因的表達(dá)情況。 方法:體外培養(yǎng)和純化大鼠BMSCs,用堿性成纖維細(xì)胞生長(zhǎng)因子(basic fibroblastgrowth factor,bFGF)和表皮生長(zhǎng)因子(epidermal growth factor,EGF)對(duì)其進(jìn)行誘導(dǎo),分別于誘導(dǎo)前和誘導(dǎo)后第七天用Affymetrix大鼠表達(dá)譜芯片檢測(cè),并且利用生物信息學(xué)分析技術(shù)比較誘導(dǎo)前后基因表達(dá)譜的改變。 結(jié)果:共有差異基因216條,其中上調(diào)基因125條,下調(diào)基因91條.與神經(jīng)元發(fā)育密切相關(guān)的基因有13條,其中上調(diào)7條,下調(diào)6條。 結(jié)論:經(jīng)體外誘導(dǎo)的BMSCs具有向神經(jīng)元分化的潛能并能表達(dá)一些與神經(jīng)元分化有關(guān)的重要基因。這些基因?qū)⑹呛罄m(xù)研究的重點(diǎn)對(duì)象。
[Abstract]:Part I changes in NSE, GFAP related mRNA and protein expression in rat bone marrow stromal cells induced by reduced glutathione
Objective: To explore the mechanism of reduced glutathione (GSH) induced differentiation of bone marrow stromalcells (BMSCs) in rats.
Methods: the rat BMSCs was cultured and purified in vitro, and was induced by GSH. After the induction of 6h, 12h, and 24h, the quantitative PCR and Westblot were measured, and the neuron specific enolase (neuron specific enolase, NSE) and glial fibrillary acidic protein were measured and the protein levels were measured and the protein levels were measured. The expression.
Results: 6h, 12h, and 24h were induced to differentiate into neuron like cells and glial cell like cells. Real-time quantitative PCR and Westblot detection results showed that the expression of NSE and GFAP in BMSCs was significantly higher than that in the control group, and the 12h was the most obvious.
Conclusion: GSH can induce BMSCs to differentiate into neuron like cells and glial cell like cells, and increase the expression of NSE and GFAP mRNA and protein after induction.
Second basic fibroblast growth factors and epidermal growth factor induced gene expression profiles of rat bone marrow stromal cells to neuron like cells
Objective: To investigate the changes in gene expression profiles of rat bone marrow stromal stem cells (BMSCs) before and after differentiation into neuron like cells, especially the expression of genes related to neuron development.
Methods: the rat BMSCs was cultured and purified in vitro. Basic fibroblastgrowth factor (bFGF) and epidermal growth factor (epidermal growth factor, EGF) were used to induce them. The expression chips were detected by Affymetrix rats before and seventh days after induction, and the bioinformatics analysis technique was used. The changes of gene expression profiles before and after induction were compared.
Results: there were 216 different genes, of which 125 were up-regulated and 91 were down regulated. There were 13 genes closely related to the development of neurons, of which 7 were up and 6 were down.
Conclusion: the in vitro induced BMSCs has the potential to differentiate into neurons and can express some important genes related to the differentiation of neurons. These genes will be the focus of follow-up research.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R329

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