發(fā)布時(shí)間：2018-06-03 23:10

  本文選題：外傷性損傷 + 5-HT_(2a)受體　； 參考：《浙江大學(xué)》2010年碩士論文

【摘要】： 金魚(Carassius auratus),現(xiàn)今作為一種模式生物已經(jīng)被廣泛應(yīng)用于神經(jīng)生理領(lǐng)域的結(jié)構(gòu)與功能研究。然而,用金魚建立損傷模型,其外傷性損傷與行為學(xué)的關(guān)系,以及損傷是否會(huì)引起特定腦區(qū)某些神經(jīng)遞質(zhì)發(fā)生神經(jīng)病理性變化,依然鮮有文獻(xiàn)報(bào)道。本實(shí)驗(yàn)旨在探討外傷性應(yīng)激對(duì)金魚造成的關(guān)于平衡和運(yùn)動(dòng)的神經(jīng)病理性影響。建立金魚剪鰭的外傷性損傷模型后,我們對(duì)金魚的行為學(xué)狀況進(jìn)行定量,發(fā)現(xiàn)5-HT_(2a)受體表達(dá)量在術(shù)后小腦區(qū)域顯著增加,在這種簡單的脊椎動(dòng)物中,證實(shí)了5-羥色胺能系統(tǒng)與行為控制之間存在關(guān)聯(lián)。同時(shí),在剪鰭金魚中發(fā)現(xiàn)有顯著的伴隨caspase-8增加的細(xì)胞凋亡加劇現(xiàn)象,可能會(huì)對(duì)金魚應(yīng)激后的自我調(diào)節(jié)機(jī)制提供新的線索,提示自我修復(fù)過程可能涉及細(xì)胞凋亡途徑。另外,我們也觀察到術(shù)后金魚的c-fos蛋白表達(dá)升高�？偟膩碚f,這些結(jié)果表明剪鰭的確能夠誘導(dǎo)行為學(xué)水平和神經(jīng)病理上的變化,說明金魚可以作為一種研究體系來探討創(chuàng)傷后應(yīng)激的潛在機(jī)制,而且這種創(chuàng)傷模型進(jìn)一步優(yōu)化建立成PTSD模型也是可行的。 此外,為了評(píng)估中樞系統(tǒng)藥物是否可以在創(chuàng)傷性損傷后發(fā)揮神經(jīng)保護(hù)作用,以及其可能的作用機(jī)制,我們分別對(duì)術(shù)后金魚給予doxepin和SSRI治療,通過檢測與損傷相關(guān)的5-羥色胺能和凋亡等指標(biāo),以觀察其是否會(huì)對(duì)該模型產(chǎn)生神經(jīng)保護(hù)作用。研究發(fā)現(xiàn),5-HT_(2a)受體的下調(diào)和5-HT_(2c)受體的上調(diào),BAD蛋白水平也下降,但是C-fos蛋白水平?jīng)]有變化,顯示創(chuàng)傷模型的行為學(xué)及神經(jīng)病理變化可以同過CNS藥物得以部分改善,而這主要基于5-羥色胺能以及細(xì)胞凋亡進(jìn)程的調(diào)節(jié)。然而,對(duì)doxepin以及SSRI治療作用的深入研究有待繼續(xù),尤其是在行為學(xué)水平。
[Abstract]:Carassius auratus, which is now widely used as a model organism, has been widely used in the study of structure and function in the field of neurophysiology. However, the use of goldfish to establish the damage model, the relationship between the traumatic injury and the behavioral science, and the neuropathic changes of certain neurotransmitters in the specific brain region, is still Xian Youwen This experiment aims to explore the neuropathic effects of traumatic stress on balance and movement caused by goldfish. After establishing a traumatic injury model of goldfish fins, we quantified the behavioral status of goldfish, and found that the expression of 5-HT_ (2a) receptor was significantly increased in the cerebellum region after the operation, in this simple vertebrate. The relationship between 5- serotonin energy system and behavioral control was confirmed. At the same time, there was a significant increase in apoptosis associated with caspase-8 in the fin goldfish, which may provide new clues to the self-regulation mechanism of the goldfish stress, suggesting that the process of self repair may involve the apoptosis pathway. In addition, we also view the apoptosis pathway. The expression of c-fos protein in goldfish was increased after the operation. In general, these results suggest that the fins can indeed induce behavioral and neuropathological changes. It shows that goldfish can be used as a research system to explore the potential mechanism of post-traumatic stress, and the further optimization of this model to establish a PTSD model is also feasible.
In addition, in order to assess whether central nervous system drugs can play a neuroprotective role after traumatic injury and its possible mechanisms of action, doxepin and SSRI were given to the goldfish after the operation, and the 5- hydroxytryptamine and apoptosis related to damage were detected to observe whether it would produce neuroprotective effects on the model. The study found that the down regulation of the 5-HT_ (2a) receptor and the up regulation of the 5-HT_ (2C) receptor and the BAD protein level also decreased, but the C-fos protein level did not change, indicating that the behavioral and neuropathological changes in the traumatic model could be partially improved with the CNS drug, which was mainly based on the regulation of 5- serotonin and the process of apoptosis. However, do Further studies on the role of xepin and SSRI remain to be continued, especially at the behavioral level.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R741;R-332

【共引文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 趙丞智;墜樓事件目擊者心理病理影響及其干預(yù)的初步探索[D];河北師范大學(xué);2003年

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本文編號(hào)：1974635