本文選題：胚胎多潛能干細(xì)胞 + 成骨細(xì)胞�。� 參考：《吉林大學(xué)》2010年碩士論文

【摘要】： 由于外傷、感染、腫瘤切除以及發(fā)育異常等原因造成的骨組織缺損的修復(fù),一直是骨科領(lǐng)域所面臨的重大問題。近年來,隨著骨組織工程研究的不斷發(fā)展,種子細(xì)胞的來源、培養(yǎng)、誘導(dǎo)、增殖、分化等研究逐漸成為骨組織工程研究的熱點。由于干細(xì)胞在特定的環(huán)境下,具有能大量增殖、分化為各種組織、器官的潛能性以及表達(dá)外源目的基因等優(yōu)點,并且可以在特定的條件下定向向成骨細(xì)胞分化。因此,利用干細(xì)胞的多向分化潛能治療骨組織損傷,有著廣闊的研究前景。 本實驗以人胚胎肝臟組織中分離出的多潛能干細(xì)胞(胎肝干細(xì)胞)為研究對象,利用維生素C、地塞米松、β-甘油磷酸鈉,在體外將其誘導(dǎo)分化為成骨細(xì)胞,并通過茜素紅染色、堿性磷酸酶染色、鈣結(jié)節(jié)染色、RT-PCR、免疫細(xì)胞化學(xué)等方法,對誘導(dǎo)后的細(xì)胞進(jìn)行鑒定,探討胎肝干細(xì)胞分化為成骨細(xì)胞的潛能及其分化機制。結(jié)果表明,體外環(huán)境下在適當(dāng)濃度的維生素C、地塞米松、β-甘油磷酸鈉的共同作用下,胎肝干細(xì)胞可以分化為成骨細(xì)胞,茜素紅染色、堿性磷酸酶染色等檢測結(jié)果呈陽性,RT-PCR檢測到成骨細(xì)胞OPN基因的表達(dá),免疫細(xì)胞化學(xué)檢測結(jié)果也呈陽性,為今后干細(xì)胞用于臨床治療骨組織缺損提供一定的理論依據(jù)和實踐依據(jù)。
[Abstract]:The repair of bone defects caused by trauma, infection, tumor excision and abnormal development has always been a major problem in orthopedics. In recent years, with the development of bone tissue engineering, the research on the origin, culture, induction, proliferation and differentiation of seed cells has gradually become a hot spot in bone tissue engineering. Stem cells have the advantages of proliferation, differentiation into various tissues, organ potential and expression of exogenous target genes in a specific environment, and can be directed to osteoblast differentiation under specific conditions. Therefore, the multidirectional differentiation potential of stem cells in the treatment of bone injury has a broad research prospect. In this study, multipotent stem cells (FHSCs) isolated from human embryonic liver tissues were induced to differentiate into osteoblasts by vitamin C, dexamethasone, 尾 -glycerophosphate in vitro and stained with alizarin red. Alkaline phosphatase staining (ALP), calcium nodule staining (RT-PCR) and immunocytochemistry were used to identify the induced cells, and to investigate the potential of fetal liver stem cells to differentiate into osteoblasts and the mechanism of differentiation. The results showed that fetal liver stem cells could differentiate into osteoblasts and stained with alizarin red under the combined action of vitamin C, dexamethasone and 尾 -glycerophosphate in vitro. The expression of OPN gene in osteoblasts was detected by RT-PCR and the results of immunocytochemistry were also positive by alkaline phosphatase staining, which provided some theoretical and practical basis for clinical treatment of bone tissue defects by stem cells.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李海紅;付小兵;張慶紅;周崗;孫同柱;;人骨髓間充質(zhì)干細(xì)胞定向誘導(dǎo)分化為脂肪細(xì)胞[J];創(chuàng)傷外科雜志;2006年01期

2 余衛(wèi),張洹,何冬梅;體外誘導(dǎo)小鼠胎肝間充質(zhì)干細(xì)胞向胰島B樣細(xì)胞分化的研究[J];第二軍醫(yī)大學(xué)學(xué)報;2004年08期

3 謝常青,林戈,盧光t;人類成纖維細(xì)胞作為人胚胎干細(xì)胞飼養(yǎng)層細(xì)胞的初步研究[J];科學(xué)通報;2002年24期

4 朱爭艷,杜智,李濤,張金卷,馬瑞麗;人胎肝干細(xì)胞的體外培養(yǎng)及誘導(dǎo)分化[J];臨床肝膽病雜志;2004年05期

5 馬兆文;吳煒;陳瑜;;人骨髓間充質(zhì)干細(xì)胞體外向肝樣細(xì)胞的誘導(dǎo)分化[J];溫州醫(yī)學(xué)院學(xué)報;2009年05期

6 孫艷;張洹;;聯(lián)合神經(jīng)生長因子誘導(dǎo)小鼠胎肝間充質(zhì)干細(xì)胞向神經(jīng)外胚層分化[J];中國臨床康復(fù);2006年09期

7 呼瑩,張麗艷,馬冠杰,姜學(xué)英,趙春華;胎兒骨髓和肝臟間充質(zhì)干細(xì)胞的表型和生物學(xué)性狀研究[J];中國實驗血液學(xué)雜志;2001年04期

8 丁亮華,羅光華,董選;成人骨髓基質(zhì)干細(xì)胞體外誘導(dǎo)成骨細(xì)胞[J];江蘇醫(yī)藥;2005年02期

9 項鵬,夏文杰,張麗蓉,陳振光,張秀明,李艷,李樹濃;成人骨髓間質(zhì)干細(xì)胞定向誘導(dǎo)為神經(jīng)元樣細(xì)胞的研究[J];中國病理生理雜志;2001年05期

10 江遜,崔鵬程,陳文弦,趙大慶,羅家勝,李貴澤,孫安;定向誘導(dǎo)人骨髓間質(zhì)干細(xì)胞向軟骨細(xì)胞分化的初步研究[J];中華耳鼻咽喉科雜志;2002年02期

，

本文編號：1970628