發(fā)布時(shí)間：2018-05-28 10:59

  本文選題：持續(xù)光照 + 恐嚇應(yīng)激�。� 參考：《南京師范大學(xué)》2008年碩士論文

【摘要】： 應(yīng)激(stress是指機(jī)體在受到各種內(nèi)外環(huán)境因素刺激時(shí)所發(fā)生的非特異性全身反應(yīng)。長(zhǎng)期處于應(yīng)激狀態(tài),機(jī)體的自穩(wěn)態(tài)將改變,從而誘發(fā)機(jī)體產(chǎn)生疾病。連續(xù)的光照刺激對(duì)內(nèi)分泌系統(tǒng)特別是垂體-性腺軸產(chǎn)生重要改變,導(dǎo)致個(gè)體體重和性腺發(fā)育出現(xiàn)異常,影響GnRH、LH分泌的強(qiáng)度和持久性。此外,長(zhǎng)日照會(huì)導(dǎo)致大鼠胰腺和小腸的淀粉酶的活性,影響消化系統(tǒng)功能。某些軍人在遭受戰(zhàn)爭(zhēng)環(huán)境刺激下會(huì)喪失應(yīng)付能力,并伴有精神障礙,稱(chēng)之為作戰(zhàn)應(yīng)激反應(yīng)(combatstress reaction CRS);發(fā)展到后期會(huì)導(dǎo)致創(chuàng)傷后應(yīng)激性障礙(post-traumaticstress disorder,PTSD)。 本實(shí)驗(yàn)?zāi)M戰(zhàn)爭(zhēng)環(huán)境建立了兩個(gè)應(yīng)激模型:持續(xù)光照應(yīng)激模型和戰(zhàn)爭(zhēng)恐懼應(yīng)激模型。分別在兩個(gè)應(yīng)激模型下檢測(cè)消化系統(tǒng)的GnRH及其受體的表達(dá)。并研究了在光照應(yīng)激模型下,大鼠性腺的發(fā)育情況。 光照應(yīng)激組大鼠睪丸重量均比對(duì)照組偏高,但差異不明顯(P＞0.05)。附睪重量與對(duì)照組相比差異不明顯(P＞0.05)。光照應(yīng)激組精子相對(duì)計(jì)數(shù)高于相應(yīng)對(duì)照組,差異顯著(P＜0.05)。 光照應(yīng)激組胃底腺壁細(xì)胞、胃小凹上皮細(xì)胞呈GnRH、GnRHR陽(yáng)性。免疫反應(yīng)陽(yáng)性細(xì)胞定位與對(duì)照組無(wú)區(qū)別。光照應(yīng)激組GnRH的平均灰度值高于相應(yīng)對(duì)照組,持續(xù)光照2w時(shí),GnRH的灰度值達(dá)到最高,光照2w到4w時(shí),實(shí)驗(yàn)組與對(duì)照組相比差異均顯著(P＜0.05)。胃的GnRHR表達(dá)量比相應(yīng)對(duì)照組升高,持續(xù)光照1w后,實(shí)驗(yàn)組與相應(yīng)的對(duì)照組相比差異顯著(P＜0.05);持續(xù)光照2w時(shí),GnRHR的表達(dá)達(dá)到最高水平,與對(duì)照組相比,差異極顯著(P＜0.01);光照3w到4w后,GnRHR的表達(dá)水平有所降低,但與相應(yīng)的對(duì)照組相比,差異仍顯著(P＜0.05)。隨著光照時(shí)間的延長(zhǎng),實(shí)驗(yàn)組大鼠GnRH和GnRHR mRNA均升高,光照應(yīng)激2w后,實(shí)驗(yàn)組GnRH mRNA與對(duì)照組相比,具有顯著性差異(P＜0.05)。在光照應(yīng)激3w后,實(shí)驗(yàn)組GnRHR mRNA與對(duì)照組相比具有顯著性差異(P＜0.05)。 恐懼應(yīng)激下,急性應(yīng)激組、慢性應(yīng)激組胃底腺壁細(xì)胞、胃小凹上皮細(xì)胞、腸黏膜層上皮細(xì)胞呈GnRHR陽(yáng)性,和相應(yīng)對(duì)照組相比,沒(méi)有明顯差異。陽(yáng)性反應(yīng)物質(zhì)定位于胞膜和胞質(zhì)中,胞核呈陰性。急性應(yīng)激條件下,大鼠胃中GnRHR表達(dá)量與對(duì)照組相比下降不顯著(P＜0.05)。慢性應(yīng)激中,GnRHR的表達(dá)量與對(duì)照組相比,差異有統(tǒng)計(jì)學(xué)意義(P＜0.01)。而在大鼠的小腸中,實(shí)驗(yàn)組大鼠比對(duì)照組大鼠GnRHR的表達(dá)量增加了29.3%。在急性應(yīng)激條件下,GnRHR表達(dá)有增多的趨勢(shì),但與對(duì)照組相比沒(méi)有顯著差異(P＞0.05);慢性應(yīng)激組與相應(yīng)的對(duì)照組相比,GnRHR表達(dá)明顯增加(P＜0.05)。 結(jié)論:持續(xù)光照對(duì)大鼠睪丸和附睪的重量無(wú)影響,但可影響精子的數(shù)量。光照應(yīng)激模型和恐懼應(yīng)激模型都能改變GnRH的分泌頻率,影響GnRH的分泌表達(dá)水平。隨著應(yīng)激時(shí)間的延長(zhǎng),消化系統(tǒng)內(nèi)GnRH及其受體的蛋白表達(dá)水平、mRNA水平也隨之變化。實(shí)驗(yàn)結(jié)果表明消化道可以產(chǎn)生GnRH,GnRH通過(guò)其受體介導(dǎo),以自分泌和旁分泌的機(jī)制對(duì)消化系統(tǒng)產(chǎn)生直接調(diào)節(jié)作用。
[Abstract]:Stress (stress refers to the non specific systemic reaction that occurs when the body is stimulated by a variety of internal and external environmental factors. For a long time in stress state, the body's self stability will change and induce the body to produce disease. Continuous illumination stimulates important changes in the endocrine system, especially the pituitary gonadal axis, leading to individual weight and gonadal glands. " Abnormal development affects the intensity and persistence of GnRH, LH secretion. In addition, long sunshine can lead to the activity of amylase in the pancreas and small intestine of the rat and affect the digestive system function. Some servicemen lose their ability to cope with war environment and have mental disorders, called combatstress reaction CRS; Post-traumaticstress disorder (PTSD) will develop later.
In this experiment, two stress models were established to simulate the war environment: the continuous light stress model and the war fear stress model. The expression of GnRH and its receptors in the digestive system were detected under two stress models, and the development of the gonadal development of rats under the light stress model was also studied.
The weight of the testis in the light stress group was higher than that in the control group, but the difference was not obvious (P > 0.05). The weight of epididymis was not significant compared with the control group (P > 0.05). The relative count of sperm in the light stress group was higher than that in the control group (P < 0.05).
Light illuminated group of gastric fundus parietal cells, gastric concave epithelial cells were GnRH, GnRHR positive. The location of immunoreactive cells was not different from that of the control group. The average gray value of GnRH in the light stress group was higher than that of the control group. The gray value of GnRH reached the highest when the light illumination was 2W, and the difference was significant between the experimental group and the control group when the light was 2W to 4W (P < 0.). 05) the expression of GnRHR in the stomach was higher than that in the corresponding control group. After 1W, the experimental group was significantly different from the corresponding control group (P < 0.05), and the expression of GnRHR reached the highest level when 2W was sustained. Compared with the control group, the difference was very significant (P < 0.01); the expression level of GnRHR decreased after the light 3W to 4W, but it was compared with the corresponding control group. The difference was still significant (P < 0.05). With the prolongation of light time, the GnRH and GnRHR mRNA in the experimental group increased, and the GnRH mRNA in the experimental group was significantly different from the control group (P < 0.05) after the photoperiod stimulated 2W (P < 0.05). The GnRHR mRNA in the experimental group was significantly different from that of the control group (P < 0.05).
In the acute stress group, the gastric fundus parietal cells, the gastric concave epithelial cells and the intestinal mucosa epithelial cells were GnRHR positive in the acute stress group and the chronic stress group. Compared with the corresponding control group, there was no significant difference. The positive reaction substance was located in the cytoplasm and cytoplasm, and the nucleus was negative. The expression of GnRHR in the stomach of the rats was compared with the control group. The decrease was not significant (P < 0.05). In the chronic stress, the expression of GnRHR was statistically significant (P < 0.01) compared with the control group (P < 0.01). In the small intestine of the rat, the expression of GnRHR in the rats of the experimental group increased the increasing tendency of 29.3%. in the acute stress condition, but there was no significant difference compared with the control group (P (0.05) compared with the corresponding control group, the expression of GnRHR increased significantly in chronic stress group (P < 0.05).
Conclusion: continuous illumination has no influence on the weight of the testis and epididymis, but affects the number of sperm. Both the light stress model and the fear stress model can change the secretion frequency of GnRH and affect the level of the expression of GnRH. As the time of stress prolongs, the protein expression level of GnRH and its receptor in the digestive system and the level of mRNA also change. The results indicate that GnRH can be produced in the alimentary tract, and GnRH mediates direct regulation of the digestive system through its receptor mediated autocrine and paracrine mechanisms.
【學(xué)位授予單位】：南京師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類(lèi)號(hào)】：R363

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