本文選題：隔藥餅灸 + 功能性消化不良��； 參考：《世界華人消化雜志》2015年19期

【摘要】：目的:觀察隔藥餅灸對功能性消化不良(functional dyspepsia,FD)肝郁脾虛模型大鼠海馬鹽皮質(zhì)激素受體(mineralocorticoid receptor,MR)、糖皮質(zhì)激素受體(glucocorticoid receptor,GR)、5-羥色胺1A受體(5-hydroxytryptamine receptor 1A,5-HTR1A)表達(dá)的影響.方法:將50只SD大鼠按隨機(jī)數(shù)字表法隨機(jī)分為空白組、模型組、隔藥餅灸組、逍遙散組、多潘立酮組5組,每組10只,空白組正常喂養(yǎng),其余4組采用復(fù)合病因造模法(慢性束縛應(yīng)激+過度疲勞+飲食失節(jié)+夾尾+搖晃)造模,連續(xù)3 wk;再按被試因素予以隔藥餅灸和逍遙散、多潘立酮灌胃,共治療2 wk.實(shí)驗(yàn)結(jié)束后采用營養(yǎng)性半固體糊灌胃法測定大鼠胃排空率,用實(shí)時(shí)熒光定量PCR(real-time fluorescence quantitative PCR,RT-PCR)法檢測大鼠海馬MR、GR、5-HTR1A的表達(dá).結(jié)果:(1)模型組大鼠胃排空率較空白組明顯下降(P0.01),治療后各治療組大鼠胃排空率較模型組上升(均P0.01),但3組間比較差異無統(tǒng)計(jì)學(xué)意義(P0.05);(2)與空白組比較,模型組大鼠MR表達(dá)差異無統(tǒng)計(jì)意義(P0.05);而G R及5-H T R1A的表達(dá)下降且差異均有統(tǒng)計(jì)意義(均P0.05);與模型組比較,隔藥餅灸組、逍遙散組、多潘立酮組大鼠海馬MR的表達(dá)亦無統(tǒng)計(jì)意義(P0.05),而GR及5-HTR1A的表達(dá)上調(diào)且差異均有統(tǒng)計(jì)學(xué)意義(均P0.05),但3組間比較差異均無統(tǒng)計(jì)學(xué)意義(P0.05).結(jié)論:隔藥餅灸同逍遙散及多潘立酮一樣能調(diào)節(jié)海馬GR、5-HTR1A的表達(dá),從而調(diào)節(jié)邊緣系統(tǒng)-下丘腦-垂體-腎上腺軸(limbic system-hypothalamic-pituitary-adrenal axis,LHPA/HPA軸),提高大鼠胃排空率,改善胃腸動力,最終起到治療本病的作用.
[Abstract]:Objective: to observe the effect of herbal cake moxibustion on the expression of 5-hydroxytryptamine receptor 1A (5-hydroxytryptamine receptor 1A) and glucocorticoid receptor (glucocorticoid receptor) in hippocampus of rats with liver stagnation and spleen deficiency model of functional dyspepsia. Methods: fifty Sprague-Dawley rats were randomly divided into blank group, model group, moxibustion group, Xiaoyao powder group, doperidone group, 10 rats in each group. The other four groups were made model by compound etiological method (chronic restraint stress excessive fatigue diet disjointed tail shake) for 3 wk, and then given moxibustion and Xiaoyao powder by medicine separated cake and Xiaoyao powder according to the factors of the test, and treated with domperidone for 2 wk. At the end of the experiment, the gastric emptying rate of rats was measured by semi-solid paste method, and the expression of 5-HTR1A in rat hippocampus was detected by real-time fluorescence quantitative PCR(real-time fluorescence quantitative PCR RT-PCR method. Results the gastric emptying rate in the model group was significantly lower than that in the blank group, and the gastric emptying rate in each treatment group was higher than that in the model group after treatment (all P 0.01, but there was no significant difference among the three groups) and there was no significant difference between the three groups and the blank group. There was no significant difference in Mr expression between model group and model group (P 0.05), but the expression of G R and 5 H T R1A decreased and the difference was statistically significant (all P 0 05); compared with the model group, the moxibustion group and Xiaoyao powder group were divided into two groups: moxibustion group and Xiaoyao powder group. There was no statistical significance in the expression of Mr in hippocampus of rats in domperidone group. However, the expression of gr and 5-HTR1A was up-regulated and the difference was statistically significant (P 0.05), but there was no significant difference among the three groups. Conclusion: herbal cake moxibustion, like Xiaoyao Powder and domperidone, can regulate the expression of GRN5-HTR1A in hippocampus, thus regulating limbic system-hypothalamic-pituitary-adrenal axis-LHPA / HPA axis of limbic hypothalamus-pituitary-adrenal axis, increasing gastric emptying rate and improving gastrointestinal motility in rats. Finally play a role in the treatment of this disease.
【作者單位】： 湖南中醫(yī)藥大學(xué);湖南中醫(yī)藥大學(xué)第二附屬醫(yī)院針灸腦病科;
【基金】：國家自然科學(xué)青年基金資助項(xiàng)目,No.81303049 湖南省中醫(yī)藥科研計(jì)劃課題基金資助項(xiàng)目,Nos.2013117,2015133 國家自然科學(xué)基金資助項(xiàng)目,No.81173326 湖南省高校創(chuàng)新平臺開放基金資助項(xiàng)目,No.12K087 高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金資助項(xiàng)目,No.20124323110001~~
【分類號】：R245.8;R-332

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