本文選題：蛋白質(zhì)組 + 雙向電泳�。� 參考：《昆明醫(yī)學(xué)院》2008年碩士論文

【摘要】： [目的]檢測貓雙側(cè)坐骨神經(jīng)切斷后所屬脊神經(jīng)節(jié)蛋白質(zhì)組的變化。 [方法]健康成年雄性貓4只,切斷雙側(cè)坐骨神經(jīng),動物存活3天后取材,分別提取脊神經(jīng)節(jié)組織總蛋白,測定其濃度。采用雙向電泳技術(shù)純化分離出蛋白質(zhì);應(yīng)用2-DE圖譜分析軟件PDQuest,分析出各組間雙向電泳圖譜中表達(dá)差異的蛋白點;選取明顯差異蛋白點做基質(zhì)輔助激光解析電離飛行時間質(zhì)譜和串聯(lián)質(zhì)譜分析,獲取肽指紋圖譜和肽序列標(biāo)簽,用Mascot軟件從NCBI數(shù)據(jù)庫中檢索確定蛋白質(zhì)名稱。 [結(jié)果]獲得重復(fù)性較好的雙向電泳圖譜,經(jīng)質(zhì)譜鑒定,坐骨神經(jīng)切斷脊神經(jīng)節(jié)組與正常對照組間存在4個差異蛋白:神經(jīng)微絲重多肽、熱休克蛋白90β、γ烯醇酶、3-磷酸甘油醛脫氫酶。此四種蛋白在坐骨神經(jīng)切斷后的所屬脊神經(jīng)節(jié)中缺失。 [結(jié)論]上述四個缺失蛋白質(zhì),可能參與了坐骨神經(jīng)切斷后引發(fā)的所屬脊神經(jīng)節(jié)的病理反應(yīng)過程。
[Abstract]:[objective] to detect the proteome changes of spinal ganglion after bilateral sciatic nerve transection in cats. [methods] the sciatic nerve was cut off from 4 healthy adult male cats. The total protein of spinal ganglion was extracted and the concentration was measured after the animals survived for 3 days. The protein was purified and separated by two-dimensional electrophoresis, and the protein spots were analyzed by 2-DE analysis software. Obviously different protein spots were selected for matrix assisted laser desorption ionization time of flight mass spectrometry and tandem mass spectrometry to obtain peptide fingerprints and peptide sequence tags. The protein names were retrieved from NCBI database by Mascot software. [results] Two-dimensional electrophoretic patterns with good reproducibility were obtained. The results showed that there were four different proteins between the sciatic nerve transection spinal ganglion group and the normal control group: neural microfilament weight polypeptide. Heat shock protein 90 尾, 緯 -enolase 3-glyceraldehyde phosphate dehydrogenase. These four proteins are absent in the spinal ganglion of the sciatic nerve. [conclusion] the above four missing proteins may be involved in the pathological reaction of the spinal ganglion after sciatic nerve transection.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R363

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