本文選題：壓力超負荷 + 去甲腎上腺素轉運蛋白�。� 參考：《河北醫(yī)科大學》2008年碩士論文

【摘要】： 目的:檢測長期心臟壓力超負荷(pressure overload,POL )大鼠心臟交感神經(jīng)去甲腎上腺素轉運蛋白(norepinephrine transporter, NET)表達與心肌神經(jīng)生長因子(nerve growth factor, NGF)表達的關系,探討NGF對NET的影響。 方法: 40只雄性Wistar大鼠隨機分為:正常組(n=10)和POL組(n=30)二組,采用腹主動脈縮窄術建立大鼠心臟POL模型。手術8周后,用超聲心電圖測定大鼠心功能指標(IVSd、IVSs、IVIDd、IVIDs、LVPWd、EDV、ESV、EF、SV、FS%),計算左心室重量指數(shù)(LVMI),觀察心臟結構變化,評價心臟功能。取左心室心肌組織切片,用免疫熒光方法觀察左心室心肌NGF蛋白表達變化。取心臟交感神經(jīng)節(jié),即雙側頸中-星狀神經(jīng)節(jié)復合體(MC-SG complex,MC-SGC),及左室心肌組織,用實時熒光定量PCR檢測MC-SGC中NET mRNA表達水平,及左室GAP43(growth associated factor, GAP43)mRNA、TrkA mRNA表達水平。 結果:與正常組比較,超聲心動圖檢測到壓力負荷組大鼠IVSs、LVPWd、IVIDs顯著增厚;LVMI升高33.23%(p0.05);心功能(EF、FS%)增強(p0.05),表明POL大鼠處于心力衰竭代償期。免疫熒光檢測結果顯示POL組心肌NGF蛋白表達增加(p0.05)。與正常對照組大鼠比較,Real Time PCR檢測POL大鼠MC-SGC中NET mRNA表達水平未見顯著變化;心肌GAP43 mRNA表達水平亦未見顯著變化,但TrKA mRNA表達水平顯著下降(p0.05)。 結論:本實驗結果表明,代償期長期壓力超負荷大鼠心肌NGF蛋白表達增加,但心肌GAP43mRNA、心臟交感神經(jīng)NET mRNA表達水平無明顯變化,TrKA mRNA表達降低。提示(1)NET mRNA轉錄后異�？赡苁菍е翽OL后NET功能異常的基礎;(2)心肌NGF表達增高未增加神經(jīng)生長,TrKA mRNA低表達水平可能是影響NGF發(fā)揮作用的重要因素。
[Abstract]:Aim: to investigate the relationship between the expression of cardiac norepinephrine transporter (NET) and the expression of nerve growth factor (NGF) nerve (NGF) nerve growth factor, NGF) in long-term cardiac pressure overload (NGF) rats, and to explore the effect of NGF on NET. Methods: forty male Wistar rats were randomly divided into two groups: normal group (n = 10) and POL group (n = 30). The rat heart POL model was established by abdominal aortic coarctation. After 8 weeks of operation, echocardiographic electrocardiogram was used to measure the cardiac function of rats. The left ventricular mass index (LVMIA) was calculated and the changes of cardiac structure and cardiac function were evaluated. The changes of NGF protein expression in left ventricular myocardium were observed by immunofluorescence. The cardiac sympathetic ganglion, MC-SG complex MC-SG complex, and left ventricular myocardial tissue were collected to detect the expression of NET mRNA in MC-SGC and the expression of GAP43(growth associated factor, GAP43mRNA-TrkA mRNA in left ventricle by real-time fluorescence quantitative PCR. Results: compared with the normal group, echocardiography showed that the IVSsl LVPWdN IVIDs were significantly thickened and LVMI was increased by 33.233p0.05, and the cardiac function was enhanced by FSI (P 0.05), indicating that the POL rats were in the compensatory phase of heart failure. The results of immunofluorescence assay showed that the expression of NGF protein increased in POL group. Compared with the control group, the expression of NET mRNA in MC-SGC of POL rats was not significantly changed by Real Time PCR, and the expression of GAP43 mRNA in myocardium was not changed significantly, but the expression of TrKA mRNA decreased significantly (p0.05). Conclusion: the results of this study indicate that the expression of NGF protein in myocardium of compensatory long-term pressure overload rats is increased, but the expression level of NET mRNA in cardiac sympathetic nerve does not change significantly. However, the expression of Trka mRNA in cardiac sympathetic nerve does not change significantly. It is suggested that the posttranscriptional abnormality of net mRNA may be the basis of NET dysfunction after POL. The increase of NGF expression in myocardium does not increase the expression of Trka mRNA, which may be an important factor affecting the role of NGF.
【學位授予單位】：河北醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R363

【參考文獻】

相關期刊論文 前1條

1 ;Norepinephrine transporter (NET) is expressed in cardiac sympathetic ganglia of adult rat[J];Cell Research;2001年04期

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本文編號：1920114