本文選題：龜芪生精膠囊 + 生精障礙�。� 參考：《昆明醫(yī)科大學(xué)》2013年碩士論文

【摘要】：[目的]建立家兔機(jī)械性生精障礙模型,通過分析家兔精液參數(shù)變化和睪丸組織學(xué)改變,觀察龜芪生精膠囊對機(jī)械性生精障礙模型家兔的促生精作用,為中藥治療男性不育提供實(shí)驗(yàn)依據(jù)。 [方法]以手術(shù)方法復(fù)制家兔單側(cè)(日本雄性大耳白兔)、雙側(cè)(雄性加利福尼亞兔)睪丸生精障礙模型,并分別根據(jù)是否進(jìn)行睪丸下降固定術(shù)予以分為不同的亞組,以黃精贊育膠囊和溫水為對照,通過縱向和橫向比較,觀察龜芪生精膠囊對各組家兔的精子密度、活力、活率和精液量的影響及睪丸組織病理變化的影響。 [結(jié)果]1.日本雄性大耳白兔單側(cè)生精障礙模型組： 1.1單側(cè)造模前后、行睪丸下降固定或不予下降處理的各組縱向比較精子密度、活力、活率均具有顯著差異(均為P0.05),精液量無顯著差異(均為P0.05),睪丸組織病理改變明顯。 1.2單側(cè)造模后行睪丸下降固定手術(shù)后4個(gè)月的各亞組(a1、a2、a3、c1、 c2、c3)與未行睪丸下降處理4個(gè)月的各亞組(b1、b2、b3、d1、d2、d3)相比較(即a1與b1、a2與b2、a3與b3、c1與d1、c2與d2、c3與d3比較)精子密度、活力、活率具有顯著差異(均為P0.05),精液量無顯著差異(均為P0.05),睪丸組織病理變化明顯。 1.3單側(cè)造模后行睪丸下降固定手術(shù)后4個(gè)月的各亞組a1、a2、a3之間及c1、c2、c3之間兩兩比較精子密度、活力、活率及精液量,a2與a1、c2與c1有顯著差異(P0.05),a3與al、c3與c1有顯著差異(P0.05),a3與a2、c3與c2無明顯差異(P0.05),各組間精液量無明顯差異(均為P0.05)。單側(cè)造模后未行睪丸下降處理4個(gè)月后的各亞組b1、b2、b3之間及d1、d2、d3之間兩兩比較精子密度、活力、活率及精液量都無明顯差異(均為P0.05)。 2.雄性加利福尼亞兔雙側(cè)生精障礙組： 2.1雙側(cè)造模前后、行睪丸下降固定或不予下降處理的各組縱向比較精子密度、活力、活率均具有顯著差異(均為P0.05),精液量無顯著差異(均為P0.05),睪丸組織病理改變明顯。 2.2雙側(cè)造模后行睪丸下降固定手術(shù)后4個(gè)月的各亞組(B1、B2、B3、C1、 C2、C3、D1、D2、D3)與未行睪丸下降處理4個(gè)月的各亞組(A1、A2、A3)相比較(即B1與A1、B2與A2、B3與A3、C1與A1、C2與A2、C3與A3、D1與A1、D2與A2、D3與A3比較)精子密度、活力、活率具有顯著差異(均為P0.05),精液量無顯著差異(均為P0.05)；雙側(cè)造模后行單側(cè)睪丸下降固定各亞組(B1、 B2、B3、C1、C2、C3)與行雙側(cè)睪丸下降固定各亞組(D1、D2、D3)相比較(即B1與Dl、B2與D2、B3與D3、C1與D1、C2與D2、C3與D3比較)精子密度、活力、活率具有顯著差異(均為P0.05),精液量無顯著差異(均為P0.05)。 2.3雙側(cè)造模后行睪丸下降固定手術(shù)后4個(gè)月的各亞組B1、B2、B3之間、C1、C2、C3之間及D1、D2、D3之間兩兩比較精子密度、活力、活率及精液量,B2與B1、C2與C1、D2與D1有顯著差異(P0.05),B3與B1、C3與C1、D3與D1有顯著差異(P0.05),B3與B2、C3與C2、D3與D2無明顯差異(P0.05),各組間精液量無明顯差異(均為P0.05)。雙側(cè)造模后未行睪丸下降處理4個(gè)月后的各亞組A1、A2、A3之間兩兩比較精子密度、活力、活率及精液量都無明顯差異(均為P0.05)。 [結(jié)論] 1.溫度因素對家兔睪丸生精障礙的影響具有一定的可逆性。 2.造成生精障礙的誘因(溫度)不解除,家兔精液質(zhì)量(精子密度、活力、活率)呈進(jìn)行性下降,睪丸組織病理變化繼續(xù)加重。 3.解除造成生精障礙的誘因(溫度)后再給予生精藥物治療能更好的促進(jìn)家兔精液質(zhì)量(精子密度、活率、活力)及睪丸組織病理變化的恢復(fù),且龜芪生精膠囊與已經(jīng)上市的國藥準(zhǔn)字號產(chǎn)品黃精贊育膠囊都有較好的療效。這為以后研究龜芪生精膠囊的作用機(jī)理以及進(jìn)行臨床試驗(yàn)奠定了實(shí)驗(yàn)基礎(chǔ)。
[Abstract]:[Objective] to establish a model of mechanical spermatogenesis disorder in rabbits. By analyzing the changes of the semen parameters and the histological changes of the testis in rabbits, the effect of turtling Qi Shengjing Capsule on the promoting spermatogenic effect of the rabbit model of mechanical spermatogenesis disorder was observed, and the experimental basis for the treatment of male infertility was provided.
[Methods] the unilateral (male, Japanese white rabbit) and bilateral (male California rabbit) model of testicular spermatogenesis were replicated by the operation method, and the testicular descent fixation was divided into different subgroups according to whether the testicular descent fixation was carried out. The effects of rabbit sperm density, viability, viability and semen volume on the pathological changes of testicular tissue were observed.
[results]1. model of unilateral spermatogenesis disorder in Japanese male white rabbits was established.
1.1 before and after the unilateral model, the sperm density, vitality and survival rate of each group with declines or no treatment were significantly different (all P0.05), and the amount of semen was not significantly different (all P0.05), and the pathological changes of the testicular tissue were obvious.
1.2 the subgroups (A1, A2, A3, C1, C2, C3) were compared with those of the subgroups (B1, B2, B3, D1, D2, D3) for 4 months after the 4 months after the unilateral model of the testicular descending operation (B1, B2, B3, D1, D2, D3). The pathological changes of the testis were obvious (all P0.05).
1.3 A1, A2, A3 and C1, C2, C3 between the subgroups of the unilateral model after the descending operation of the testis, and C1, C2, and C3, compared with the sperm density, vigor, the viability and the semen quantity, A2 and A1, C2 and C1 significant difference (P0.05). All of the subgroups of B1, B2, B3 and D1, D2, D3 after 4 months after the unilateral model of the unilateral model were compared with D1, D2, and D3, and there were no significant differences in sperm density, activity, viability and semen quantity (P0.05).
2. male California rabbit bilateral spermatogenic disorder group:
2.1 there were significant differences in sperm density, vitality and survival rate (all P0.05), and no significant difference in semen volume (all P0.05), and the pathological changes of testicular tissue were obvious in each group.
2.2 compared with each subgroup (B1, B2, B3, C1, C2, C3, D1, D2, D3) in the 4 months after the bilateral testicular descending operation (D1, D2, D3) in each subgroup (A1, A2, A3) There was no significant difference in semen volume (all P0.05), and bilateral testicular descending groups (B1, B2, B3, C1, C2, C3) were compared with each subgroup (D1, D2, D3) of bilateral testicular descent (D1, D2, D3) in bilateral spermatozoa after bilateral model building. Significant differences (all P0.05).
2.3 B1, B2, B3, C1, C2, C3, and D1, D2, and D3 were compared with D1, D2, and D3, compared with D1, D2, and D3, and there were significant differences between B2 and B1 (B2 and B1). 5) there was no significant difference between the semen of each group (all P0.05). There was no significant difference in sperm density, activity, viability and semen amount (all P0.05) between the subgroups of A1, A2, and A3 after 4 months after bilateral modeling.
[Conclusion]
1. the influence of temperature on spermatogenesis impairment of rabbit testis is reversible.
2. the semen quality (sperm density, vitality, viability) decreased progressively, and the pathological changes of testicular tissue continued to increase.
3. relieving the cause of spermatogenesis disorder (temperature) and giving the treatment of spermatogenic drugs can better promote the quality of the rabbit's semen (sperm density, survival rate, vitality) and the recovery of the pathological changes of the testis. The mechanism of Shengjing capsule and its clinical trials have laid an experimental foundation.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R277.5;R-332

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