本文選題：SD大鼠 + 模型��； 參考：《復(fù)旦大學(xué)》2008年碩士論文

【摘要】： 目的: 建立兩種SD大鼠慢性非細(xì)菌性前列腺炎的模型,研究其生物學(xué)特性,探討慢性非細(xì)菌性前列腺炎的發(fā)病機(jī)制,觀察模型對(duì)生殖功能的影響,觀察舍尼通對(duì)模型的療效,探討舍尼通的作用機(jī)理。 方法: 1、模型一:自體免疫性慢性非細(xì)菌性前列腺炎模型的建立(簡稱免疫性模型) 32只大鼠隨機(jī)分成4組,其中一組為陰性對(duì)照組,其它三組為試驗(yàn)組,第0、7天多點(diǎn)皮內(nèi)注射濃度分別為20、40、80mg/ml的大鼠前列腺蛋白提純液聯(lián)合完全弗氏佐劑(1:1的混懸液)1.0ml,腹腔注射百白破疫苗0.5ml,第21天腹腔注射大鼠前列腺蛋白提純液0.5ml,陰性對(duì)照組在相同時(shí)間和部位注射等量的生理鹽水,造模后第35天處死大鼠。 2、模型二:化學(xué)性慢性非細(xì)菌性前列腺炎模型的建立(簡稱化學(xué)性模型) 16只大鼠隨機(jī)分為兩組,陰性對(duì)照組和模型組。模型組用1%λ-角叉菜膠生理鹽水溶液0.1ml直接注射前列腺內(nèi),陰性對(duì)照組注射生理鹽水。造模后第35天處死大鼠。 3、觀察大鼠的前列腺大體和組織病理學(xué)變化,評(píng)價(jià)模型是否成功,并測定血清中的Ca~(2+)、Mg~(2+)、雌二醇(E_2)、睪酮(T)、雙氫睪酮(DHT)含量,觀察其生物學(xué)特性變化,初步探討慢性非細(xì)菌性前列腺炎的發(fā)病機(jī)制。 4、造模成功后,雌雄鼠1:1交配,觀察雌鼠的妊娠率,仔鼠的生長發(fā)育,評(píng)價(jià)模型對(duì)生殖功能的影響。 5、用陽性藥舍尼通模擬臨床途徑灌胃給藥,觀察舍尼通對(duì)兩種模型的療效,初步探討舍尼通的作用機(jī)理。 結(jié)果: 1、在大鼠前列腺蛋白提純液聯(lián)合雙重免疫佐劑制作的SD大鼠自體免疫性前列腺炎模型中,高劑量(80mg/ml)模型組的前列腺部分腺腔破壞,明顯的充血、水腫,間質(zhì)和腺腔有淋巴細(xì)胞和單核細(xì)胞浸潤,呈現(xiàn)明顯的慢性炎癥表現(xiàn)。 2、在SD大鼠前列腺內(nèi)注射1%λ-角叉菜膠生理鹽水溶液制作的化學(xué)性前列腺炎模型中,觀察到前列腺間質(zhì)有大量淋巴細(xì)胞和單核細(xì)胞侵潤,血管增生和充血明顯,呈現(xiàn)明顯的慢性炎癥表現(xiàn)。 3、在免疫模型組中,與陰性對(duì)照組相比,高劑量模型組的血液白細(xì)胞數(shù)明顯升高(P≤0.05):低、中劑量模型組的血清Ca~(2+)升高,但差別不顯著(P＞0.05),高劑量模型組的血清Ca~(2+)明顯降低(P≤0.05);低、中、高劑量模型組的血清IgG均明顯升高(P≤0.01);低劑量模型組的血清T明顯升高(P≤0.05),中、高劑量模型組的血清T也升高,但差別不顯著(P＞0.05);中、高劑量模型組的DHT升高,且高劑量模型組升高的幅度較大,但差別不顯著(P＞0.05)。 4、在1%角叉菜膠模型組中,與陰性對(duì)照組相比,模型組的血清雙氫睪酮含量明顯降低(P≤0.01);前列腺臟器系數(shù)明顯下降,說明前列腺重量明顯減輕(P≤0.05);血清中的T、Ca~(2+)和Mg~(2+)濃度升高,但差別不顯著(P＞0.05)。 5、兩種模型對(duì)生殖的影響:精子活力分析結(jié)果為:A、B、D級(jí)活力的精子:陰性對(duì)照組與1%角叉菜膠模型組之間有顯著差異(P≤0.05),C級(jí)活力的精子:陰性對(duì)照組與1%角叉菜膠模型組之間無顯著差異(P≥0.05),各級(jí)活力的精子在陰性對(duì)照組和免疫模型組之間均無顯著差異(P≥0.05);雌鼠妊娠率、產(chǎn)仔數(shù)和仔鼠出生的性別比例及外觀畸形數(shù)和死亡數(shù):三組之間無明顯差別;仔鼠的前列腺病理學(xué)表現(xiàn):與陰性對(duì)照組相比,模型組的仔鼠前列腺無明顯改變,都是光滑、無結(jié)節(jié),無充血水腫,無炎細(xì)胞侵潤。 6、舍尼通對(duì)兩種模型的作用:對(duì)免疫模型的作用表現(xiàn)在顯著升高血清中的Ca~(2+)水平和T水平(P≤0.05);對(duì)化學(xué)模型的作用表現(xiàn)在用藥后血液中的白細(xì)胞數(shù)顯著下降(P≤0.05):對(duì)前列腺的組織病理學(xué)改變都顯示出充血水腫減輕。 結(jié)論: 1、第0、7天多點(diǎn)皮內(nèi)注射濃度為80mg/ml的大鼠前列腺蛋白提純液聯(lián)合完全弗氏佐劑(1:1的混懸液),腹腔注射百白破疫苗0.5ml,第21天腹腔注射大鼠前列腺蛋白提純液0.5ml,造模35天可以成功制作SD大鼠自體免疫性慢性前列腺炎的模型。 2、采用1%λ-角叉菜膠生理鹽水溶液0.1ml直接注射前列腺內(nèi),造模35天可以成功制作SD大鼠化學(xué)性慢性非細(xì)菌性前列腺炎的模型。 3、化學(xué)性模型能明顯降低精子活力,兩種模型對(duì)雌鼠妊娠率、仔鼠的生長發(fā)育無明顯影響。 4、舍尼通對(duì)兩種慢性前列腺炎均有效,但作用機(jī)制不同。對(duì)免疫模型是通過升高Ca~(2+)和T的水平,對(duì)化學(xué)性模型是通過減少白細(xì)胞數(shù)來起作用。
[Abstract]:Objective:
To establish a model of two chronic non bacterial prostatitis in SD rats, study its biological characteristics, explore the pathogenesis of chronic non bacterial prostatitis, observe the effect of the model on the reproductive function, observe the effect of the model and explore the mechanism of the action of the model.
Method:
1, model 1: establishment of autoimmune chronic nonbacterial prostatitis model (Immune Model).
32 rats were randomly divided into 4 groups. One group was a negative control group and the other three groups were in the experimental group. The rat prostatic protein purify solution (20,40,80mg/ml) combined with complete Freund's adjuvant (1:1 suspension) 1.0ml, intraperitoneal injection of pestilence 0.5ml in the abdominal cavity and intraperitoneal injection of prostatic protein purification in the abdominal cavity on day twenty-first. The rats in the negative control group were injected with the same amount of normal saline at the same time and place, and rats were killed thirty-fifth days after modeling. 0.5ml
2, model two: establishment of a chemical chronic non bacterial prostatitis model (chemical model).
16 rats were randomly divided into two groups, the negative control group and the model group. The model group was injected with 1% lambda carrageenan physiological saline solution 0.1ml directly into the prostate, and the negative control group was injected with saline. The rats were killed thirty-fifth days after building the model.
3, observe the general and histopathological changes of the prostate in rats, evaluate the success of the model, and determine the content of Ca~ (2+), Mg~ (2+), estradiol (E_2), testosterone (T), and dihydrotestosterone (DHT) in the serum, and observe the changes in the biological characteristics of the rats. The pathogenesis of chronic non bacterial prostatitis is preliminarily discussed.
4, after successful modeling, male and female mice 1:1 mating, observe the pregnancy rate of female rats, the growth and development of offspring rats, and evaluate the effect of the model on reproductive function.
5, we used the positive drug Shinto Tong to simulate the clinical route of gavage, observe the curative effect of shinton on the two models, and preliminarily explore the mechanism of the effect of shinby.
Result:
1, in the SD rat autoimmune prostatitis model of rat prostatic protein purification fluid combined with double immuno adjuvant, the partial adenocarcinoma of the prostate in the high dose (80mg/ml) model group was obviously congested, edema, interstitial and adenocavities were infiltrated with lymphocytes and mononuclear cells, showing obvious chronic inflammatory performance.
2, in the chemical prostatitis model produced by injection of 1% lambda - carrageenan saline solution in the prostate of SD rats, a large number of lymphocytes and monocytes were infiltrated, vascular proliferation and hyperemia were evident in the prostatic interstitium, showing obvious chronic inflammatory manifestations.
3, in the immune model group, the number of white blood cells in the high dose model group was significantly higher than that in the negative control group (P < 0.05). The serum Ca~ (2+) in the middle dose model group increased, but the difference was not significant (P > 0.05). The serum Ca~ (2+) of the high dose model group decreased significantly (P < 0.05), and the serum IgG in the high dose model group increased significantly (P). In the low dose model group, the serum T increased significantly (P < 0.05). In the high dose model group, the serum T increased, but the difference was not significant (P > 0.05). In the high dose model group, the DHT increased, and the high dose model group increased significantly, but the difference was not significant (P > 0.05).
4, in the 1% corner carrageenan model group, the serum dihydrotestosterone content of the model group was significantly lower than that of the negative control group (P < 0.01), and the prostate organ coefficient decreased significantly, indicating that the prostate weight was significantly reduced (P < 0.05), and the concentration of T, Ca~ (2+) and Mg~ (2+) in the serum was increased, but the difference was not significant (P > 0.05).
5, the effect of two models on reproduction: sperm motility analysis results were: A, B, D class vitality sperm: negative control group and 1% carrageenan model group significant difference (P < 0.05), C class vitality sperm: negative control group and 1% corner carrageenan model group no significant difference (P > 0.05), the vitality of sperm at all levels in negative control and immunization There was no significant difference between the two groups (P > 0.05). The pregnancy rate, the number of offspring and the sex ratio of the offspring, the number of deformities and the number of deaths were no significant difference between the three groups. No inflammatory cells invade.
6, the effect of seronont on the two models: the effect on the immune model was shown to significantly increase the level of Ca~ (2+) and T (P < 0.05) in the serum; the effect of the chemical model on the number of white blood cells in the blood decreased significantly (P < 0.05) after the drug use: the histopathological changes in the prostatic group showed the reduction of congestion and edema.
Conclusion:
1, on day 0,7, the rat prostatic protein purify solution with intradermal injection of 80mg/ml combined with complete Freund's adjuvant (1:1 suspension), intraperitoneal injection of pertussis vaccine 0.5ml, and intraperitoneal injection of prostatic protein purified liquid 0.5ml in rats on the twenty-first day, and the model of autoimmune chronic prostatitis in SD rats could be successfully made for 35 days.
2, 1% lambda carrageenan physiological saline solution 0.1ml was injected into the prostate directly. The model of chemical chronic non bacterial prostatitis in SD rats could be successfully made for 35 days.
3, the chemical model can significantly reduce sperm motility, and the two models have no significant effect on the pregnancy rate and the growth and development of offspring rats.
4, all of the two kinds of chronic prostatitis were effective, but the mechanism was different. The immune model was used to increase the level of Ca~ (2+) and T, and the chemical model was used to reduce the number of white cells.
【學(xué)位授予單位】：復(fù)旦大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R-332;R697.33

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 薛博;丹蒲膠囊對(duì)實(shí)驗(yàn)性自身免疫前列腺炎大鼠Th1/Th2平衡及Treg細(xì)胞的影響[D];北京中醫(yī)藥大學(xué);2010年

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本文編號(hào)：1902140