本文選題：慢性間歇性缺氧 + 氧化應激　； 參考：《重慶醫(yī)科大學》2010年碩士論文

【摘要】： 目的： 制作可應用于常壓慢性間歇性缺氧研究的動物模型,為進一步研究OSAHS (Obstructive sleep apnea-hypopnea syndrome)的生理病理提供一個實驗平臺。通過研究正常和慢性間歇性缺氧以及去除缺氧后大鼠心肌還原型谷胱甘肽(GSH)和蛋白質(zhì)羰基化(PC)的變化來探討OSAHS患者心肌是否存在氧化應激(OS)及氧化應激引起的進一步損害。 方法： 選取24只SD健康成年雄性大鼠,隨機分為空白組(unhandled control, UC組)、慢性間歇性缺氧組(chronic intermittent hypoxia,CIH組)和去除缺氧組(Removal of hypoxia, RH組),UC組正常飼養(yǎng),CIH組每日間歇缺氧8小時,連續(xù)4周,建立慢性間歇性缺氧模型,RH組前4周同CIH組,后4周正常飼養(yǎng),所有大鼠在實驗結束后取心肌0.5g再提取心肌蛋白進行免疫印跡來分析還原型谷胱甘肽(GSH)和蛋白質(zhì)羰基化(PC)的變化。 結果： ①GSH的表達CIH組較UC組和RH組明顯降低(p0.05),而UC組和RH組之間無明顯差異(p0.05)。 ②PC的結果CIH組較UC組和RH組明顯升高(p0.05),而UC組和RH組之間無明顯差異(p0.05)。 結論： 慢性間歇性缺氧致使大鼠心肌存在氧化應激狀態(tài),并引起心肌蛋白的損傷。
[Abstract]:Purpose :



To establish an animal model applicable to the study of chronic intermittent hypoxia in normal pressure , and to provide an experimental platform for further study of the physiological and pathological features of obstructive sleep apnea - syndrome syndrome . By studying the changes of normal and chronic intermittent hypoxia and the changes of glutathione ( GSH ) and protein carbonylation ( PC ) in rats after hypoxia , it is possible to study the presence of oxidative stress ( OS ) and oxidative stress in the myocardium of patients with obstructive sleep apnea .



Method :



24 SD healthy adult male rats were randomly divided into blank group ( UC group ) , chronic intermittent hypoxia group ( group C ) , chronic intermittent hypoxia group ( CIH group ) and hypoxia group ( removal of hypoxia , RH group ) .



Results :



The expression of GSH increased significantly in CIH group than in UC group and RH group ( p < 0.05 ) , but there was no significant difference between UC group and RH group ( p < 0.05 ) .



The results of PC showed that the CIH group was significantly higher than that in UC group and RH group ( p < 0.05 ) , but there was no significant difference between UC group and RH group ( p < 0.05 ) .



Conclusion :



Chronic intermittent hypoxia causes oxidative stress in the myocardium of rats and causes damage to myocardial proteins .

【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R363

【參考文獻】

相關期刊論文 前7條

1 盧紅霞,劉長梅;阻塞性睡眠呼吸暫停綜合征患者血中WBC數(shù)量分析[J];山東醫(yī)大基礎醫(yī)學院學報;2000年06期

2 陸怡，，潘華珍，許彩民;氧化與細胞凋亡[J];生物化學與生物物理進展;1996年02期

3 張佳賓,余勤,劉曉菊;睡眠呼吸監(jiān)測期間猝死1例[J];中國實用內(nèi)科雜志;2004年07期

4 劉輝國,劉瑾,熊盛道,沈關心,張珍祥,徐永健;The Change of Interleukin-6 and Tumor Necrosis Factor in Patients with Obstructive Sleep Apnea Syndrome[J];Journal of Tongji Medical University;2000年03期

5 張潔心;殷國勇;陸甘;;阻塞性睡眠呼吸暫停綜合征與氧化應激研究進展[J];江蘇醫(yī)藥;2008年11期

6 馮學威,康健,王贊峰,王瑋,于潤江;睡眠中間斷低氧對大鼠下丘腦-垂體-腎上腺軸和生長激素的影響[J];中國應用生理學雜志;2005年04期

7 陳萍;鄧茂先;黃文婷;;睡眠呼吸暫�；颊呃唛g外肌氧化應激的研究[J];中國現(xiàn)代醫(yī)藥雜志;2008年04期

本文編號：1897235