本文選題：慢性牙周炎 + 動脈粥樣硬化��； 參考：《新疆醫(yī)科大學》2009年碩士論文

【摘要】： 目的:選取Wistar大鼠為實驗對象,通過建立慢性牙周炎和動脈粥樣硬化結(jié)合的動物模型并進行相關(guān)性的初步分析,探討慢性牙周炎和動脈粥樣硬化發(fā)病相關(guān)的可能機制。方法:雄性Wistar大鼠(體重200±20g)60只隨機分為四組:A對照組,B慢性牙周炎組,C動脈粥樣硬化組,D慢性牙周炎+動脈粥樣硬化組。各組按照實驗設計進行相應干預處理,至12周末處死動物,分別檢查記錄牙周指標,檢測血清中的血脂水平,酶聯(lián)免疫吸附法(ELISA)檢測血清中的腫瘤壞死因子α(TNF-α)以及基質(zhì)金屬蛋白酶-3(MMP-3)的含量,免疫組化技術(shù)分別檢測MMP-3在牙周和腹主動脈組織中的表達,并進行圖像半定量分析,同時觀察牙周和腹主動脈的組織病理學改變。數(shù)據(jù)進行統(tǒng)計學分析。結(jié)果:經(jīng)過牙周檢查、血脂、ELISA檢測以及組織病理學觀察,已成功建立了動物模型。其中D組的血脂水平、血清TNF-α及MMP-3含量明顯升高,各實驗組與對照組比較差異均有統(tǒng)計學意義(P0.05)。經(jīng)過免疫組化檢測發(fā)現(xiàn),MMP-3在B組和D組的牙周組織中,主要在牙齦上皮呈陽性表達,并且D組水平高于B組;在實驗組的腹主動脈中,主要在內(nèi)皮細胞、巨噬細胞、平滑肌細胞呈陽性表達,并且D組水平高于C組,差異均有統(tǒng)計學意義(P0.05)。MMP-3在組織中的表達與血清中的規(guī)律相一致。MMP-3與血脂的相關(guān)性分析呈現(xiàn)出不相關(guān)。結(jié)論:慢性牙周炎與動脈粥樣硬化發(fā)病可能具有一定的相關(guān)性,可能對動脈粥樣硬化和冠心病的發(fā)生發(fā)展有重要的影響。MMP-3是獨立于血脂之外的動脈粥樣硬化危險因素。
[Abstract]:Objective: to investigate the possible mechanism of chronic periodontitis and atherosclerosis by establishing the animal model of chronic periodontitis and atherosclerosis in Wistar rats. Methods: male Wistar rats (body weight 200 鹵20g)60) were randomly divided into four groups: control group (n = 4) and chronic periodontitis group (n = 4). According to the experimental design, the animals were killed at the end of 12 weeks. The periodontal indexes were recorded and the serum lipids were measured. Serum levels of TNF- 偽 and matrix metalloproteinase-3 (MMP-3) were detected by Elisa, and the expression of MMP-3 in periodontal and abdominal aorta was detected by immunohistochemistry. The histopathological changes of periodontal and abdominal aorta were observed. The data were analyzed statistically. Results: the animal model was successfully established by periodontal examination, Elisa and histopathological observation. The levels of blood lipid, TNF- 偽 and MMP-3 in group D were significantly higher than those in control group (P 0.05). The expression of MMP-3 in the periodontal tissues of group B and group D was mainly positive in gingival epithelium, and the level of MMP-3 in group D was higher than that in group B. in the abdominal aorta of experimental group, the expression of MMP-3 was mainly in endothelial cells and macrophages. The expression of smooth muscle cells was positive and the level of group D was higher than that of group C. the difference was statistically significant. The expression of P0.05U. MMP-3 was consistent with the rule of serum. There was no correlation between MMP-3 and serum lipids. Conclusion: chronic periodontitis may be associated with the pathogenesis of atherosclerosis, which may have an important effect on the occurrence and development of atherosclerosis and coronary heart disease. MMP-3 is a risk factor of atherosclerosis independent of blood lipids.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R781.4;R543.5;R-332

【參考文獻】

相關(guān)期刊論文 前10條

1 馬寧,張穎麗,張莉,高文信,孫鳳丹;牙周炎動物模型的研究[J];白求恩醫(yī)科大學學報;2001年06期

2 羅云綱,楊濤,許力強,黃洋;牙周炎動物模型的建立[J];吉林大學學報(醫(yī)學版);2004年02期

3 楊鵬遠,芮耀誠,焦亞斌;動脈粥樣硬化大鼠實驗模型的建立[J];第二軍醫(yī)大學學報;2003年07期

4 張磊,陳國榮,鄭榮遠,王群姬,謝淑萍,王擁軍;高脂飼料加空氣干燥術(shù)建立兔頸動脈粥樣硬化模型[J];中國動脈硬化雜志;2001年02期

5 金海燕;冬畢華;彭曠;孫慧;葉旭;楊永宗;;動脈粥樣硬化發(fā)生中高脂血癥與動脈狹窄的作用比較[J];中國動脈硬化雜志;2006年12期

6 彭式韞;周以鈞;陳美美;吳織芬;汪阿冬;楊連甲;孫慶妹;;牙周炎動物模型及其翻瓣術(shù)的愈合情況(猴牙實驗研究)[J];口腔醫(yī)學;1990年04期

7 李永明,林珠,段銀鐘;IL-6體外誘導成年大鼠骨髓破骨細胞形成的實驗觀察[J];口腔醫(yī)學縱橫;2001年01期

8 陳鐵樓，李秦，周以鈞，，吳織芬，金巖，劉鍵;大鼠實驗性牙周炎動物模型研究[J];臨床口腔醫(yī)學雜志;1996年04期

9 劉文衛(wèi);劉永勝;江華;朱銳;湯永謙;李彬;馬可中;葉劍文;李素珍;李智山;;急性冠狀動脈綜合征患者血清白細胞介素-10、18及腫瘤壞死因子α水平及其臨床意義[J];臨床心血管病雜志;2006年12期

10 張明珠;梁景平;李超倫;姜云濤;束蓉;;牙齦卟啉單胞菌感染兔動脈粥樣硬化模型的建立[J];上海交通大學學報(醫(yī)學版);2007年06期

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