本文選題：低氧/復(fù)氧損傷 + 堿性成纖維細(xì)胞生長(zhǎng)因子��； 參考：《蘭州大學(xué)》2010年碩士論文

【摘要】： 目的：探討堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)對(duì)心臟成纖維細(xì)胞低氧／復(fù)氧損傷后Wnt通路中β-catenin蛋白表達(dá)的調(diào)節(jié)作用及其機(jī)制。 方法：采用物理缺氧法制作體外培養(yǎng)SD大鼠心臟成纖維細(xì)胞低氧／復(fù)氧損傷模型。將細(xì)胞隨機(jī)分6組(1)對(duì)照組：細(xì)胞置于常氧培養(yǎng)箱內(nèi)持續(xù)孵育。(2)低氧／復(fù)氧組(損傷組)：細(xì)胞置于低氧培養(yǎng)箱中低氧培養(yǎng)24小時(shí),然后置于常氧培養(yǎng)箱中復(fù)氧培養(yǎng)半小時(shí)。(3-6)堿性成纖維細(xì)胞生長(zhǎng)因子+低氧／復(fù)氧組(堿性成纖維細(xì)胞生長(zhǎng)因子干預(yù)組),細(xì)胞低氧／復(fù)氧后以堿性成纖維細(xì)胞生長(zhǎng)因子(basic fibroblast growth factor, bFGF)處理(堿性成纖維細(xì)胞生長(zhǎng)因子濃度5μg/L、10μg/L、20μg/L、40μg/L)。實(shí)驗(yàn)采用采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴鹽(MTT)比色法、末端脫氧核糖核酸轉(zhuǎn)移酶介導(dǎo)的dUTP切口末端標(biāo)記(TUNEL)法、免疫熒光法分析檢測(cè)心臟成纖維細(xì)胞相對(duì)活力、凋亡百分率、P-catenin蛋白的平均熒光值。 結(jié)果：SD (Sprague Dawley)大鼠低氧／復(fù)氧后MTT檢測(cè)結(jié)果提示堿性成纖維細(xì)胞生長(zhǎng)因子+低氧／復(fù)氧組較低氧／復(fù)氧損傷組增值率增加(P0.05),而激光共聚焦顯微鏡結(jié)果提示堿性成纖維細(xì)胞生長(zhǎng)因子+低氧／復(fù)氧組β-catenin蛋白平均熒光值高于低氧／復(fù)氧損傷組(P0.05),β-catenin蛋白增多；TUNEL陽(yáng)性反應(yīng)產(chǎn)物百分比較低氧／復(fù)氧損傷組減少(P0.05)。 結(jié)論：堿性成纖維細(xì)胞生長(zhǎng)因子能夠減少低氧／復(fù)氧損后心臟成纖維細(xì)胞凋亡,并參與了Wnt通路中β-catenin蛋白表達(dá)的調(diào)節(jié),對(duì)低氧／復(fù)氧損傷后的心臟成纖維細(xì)胞具有促進(jìn)增殖作用。
[Abstract]:Aim: to investigate the effect of basic fibroblast growth factor (bFGF) on the expression of 尾 -catenin protein in Wnt pathway of cardiac fibroblasts after hypoxia / reoxygenation injury. Methods: hypoxia / reoxygenation injury model of rat cardiac fibroblasts in vitro was established by physical hypoxia. The cells were randomly divided into 6 groups: control group: the cells were incubated in a normoxic incubator for 24 hours in hypoxia / reoxygenation group (injury group: cells were incubated in hypoxia incubator for 24 hours). Then incubated in a normoxic incubator for half an hour after reoxygenation, the basic fibroblast growth factor hypoxia / reoxygenation group (basic fibroblast growth factor intervention group), cells hypoxia / reoxygenation after the basic fibroblast growth factor growth Basic fibroblast growth factor (bFGF) treatment (concentration of basic fibroblast growth factor 5 渭 g / L ~ 10 渭 g / L ~ (10) 渭 g / L ~ (20) 渭 g / L ~ (20) 渭 g / L ~ (40) 渭 g / L ~ (-1)). The relative viability of cardiac fibroblasts was detected by the method of 3- (4-dimethylthiazole-2-dimethylthiazolium) -2-diphenyltetrazolium bromide (MTT) colorimetry, terminal deoxyribonucleic acid transferase-mediated dUTP Nick end labeling (Tunel) method, and immunofluorescence assay. Percentage of apoptosis and average fluorescence value of P-catenin protein. Results the results of MTT detection after hypoxia / reoxygenation showed that the proliferation rate of basic fibroblast growth factor hypoxia / reoxygenation group was higher than that of hypoxia / reoxygenation injury group, while the results of laser confocal microscopy showed that basic fibroblast growth factor (bFGF) fibroblast growth factor in hypoxia / reoxygenation group was higher than that in hypoxia / reoxygenation group. The average fluorescence value of 尾 -catenin protein in the hypoxia / reoxygenation group was higher than that in the hypoxia / reoxygenation group, and the percentage of Tunel positive reaction products in the hypoxia / reoxygenation group was lower than that in the hypoxia / reoxygenation group. Conclusion: basic fibroblast growth factor can reduce the apoptosis of cardiac fibroblasts after hypoxia / reoxygenation, and participate in the regulation of 尾 -catenin protein expression in Wnt pathway. It can promote the proliferation of cardiac fibroblasts after hypoxia / reoxygenation injury.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 嚴(yán)俊,高鈺琪,張恒,王培勇,謝增柱;低氧促進(jìn)大鼠心臟成纖維細(xì)胞膠原蛋白合成[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2005年19期

2 林嵐,洪華山,王一波,江瓊;體外培養(yǎng)心臟成纖維細(xì)胞增殖的檢測(cè)方法[J];福建醫(yī)科大學(xué)學(xué)報(bào);2000年01期

3 林灼鋒,李校坤,鄭青,黃亞?wèn)|,孟娟;酸性成纖維細(xì)胞生長(zhǎng)因子在培養(yǎng)的乳鼠心肌細(xì)胞中分泌表達(dá)及其意義[J];中國(guó)藥科大學(xué)學(xué)報(bào);2004年02期

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本文編號(hào)：1857151