本文選題：四硝基喹啉-1-氧化物 + 口腔癌�。� 參考：《廣西醫(yī)科大學(xué)》2013年碩士論文

【摘要】：研究目的：(1).通過比較四硝基喹啉-1-氧化物(4-nitroquinoline1-oxide,4-NQO)飲水法在不同給藥時間誘發(fā)小鼠口腔癌淋巴道轉(zhuǎn)移模型過程中口腔黏膜病變程度的差異,進(jìn)一步改進(jìn)小鼠口腔癌淋巴道轉(zhuǎn)移模型。 (2).利用4-硝基喹啉-1-氧化物(4-nitroquinoline1-oxide,4-NQO)飲水法誘發(fā)小鼠口腔癌模型,提取不同時期的骨髓播散細(xì)胞,比較口腔黏膜癌變過程不同時期骨髓播散細(xì)胞的差異。 研究方法：(1).70只balb/c小鼠,隨機(jī)分為2組：實(shí)驗(yàn)組60只,給予200mg/L4NQ0飲水喂養(yǎng)8、14、20周(各20只),停藥改用普通自來水喂養(yǎng)到45周；對照組10只小鼠,單純飲用自來水。45周時通過肉眼和組織學(xué)觀察各組其病變情況并比較小鼠體重的變化情況。 (2).通過第一部分完善構(gòu)建動物模型的方法構(gòu)建小鼠口腔癌變動物模型,選擇病理檢查明確診斷為正常舌粘膜的小鼠10例、舌單純性增生的小鼠10例、舌輕中度異常增生的小鼠20例、舌重度異常增生的小鼠20例、舌高分化鱗癌的小鼠20例。收集所有小鼠的后腿股骨骨髓,用Ficoll密度梯度離心法提取單個核細(xì)胞并制成細(xì)胞涂片后,以PAN-CK上皮性特異性抗體為標(biāo)記物,用免疫細(xì)胞化學(xué)SP法鑒定骨髓播散上皮細(xì)胞,比較不同時期的骨髓播散細(xì)胞的數(shù)目差異。 結(jié)果：(1).隨著給藥時間的延長,實(shí)驗(yàn)組小鼠舌背黏膜相繼出現(xiàn)白色斑塊、潰瘍、乳頭狀增生等改變,小鼠舌背產(chǎn)生輕度異常增生—中度異常增生—重度異常增生—原位癌—鱗癌的典型病理變化。給藥8周組70.6%小鼠舌背黏膜表現(xiàn)為單純性增生,29.4%為輕中重度異常增生,舌癌發(fā)生率為0%；給藥14周組88.2%為輕中重度異常增生,11.8%為原位癌；給藥20周組100%為鱗癌,并且100%轉(zhuǎn)移頸部淋巴結(jié)；三組未見遠(yuǎn)處轉(zhuǎn)移。14、20周組小鼠體重較給藥前明顯減輕(p0.05),8周組和對照組小鼠體重較給藥前無明顯變化(p0.05)。 (2).10例正常舌粘膜和10例單純性增生,20例輕中度異常增生的小鼠骨髓細(xì)胞涂片均未能發(fā)現(xiàn)播散陽性細(xì)胞,而在20例舌重度異常增生的小鼠骨髓細(xì)胞涂片中有8例發(fā)現(xiàn)播散陽性細(xì)胞,占40%；20例鱗癌小鼠骨髓涂片中有13例發(fā)現(xiàn)播散陽性細(xì)胞,占65%。重度異常增生、鱗癌組兩組之間播散細(xì)胞陽性率的比較有顯著性差異(P0.05)。并發(fā)現(xiàn)骨髓播散細(xì)胞陽性組之間,隨著小鼠舌黏膜逐步惡變,骨髓中的播散細(xì)胞數(shù)越多。 結(jié)論：(1).在相同劑量下,隨著給藥時間延長,口腔黏膜癌前病變以及口腔癌發(fā)生率越高,但是體重減輕越明顯。 (2).隨著小鼠舌黏膜逐步惡變,在舌重度異常增生時,已經(jīng)出現(xiàn)骨髓播散細(xì)胞,并且隨著病變程度加重,骨髓播散細(xì)胞發(fā)生率和骨播散細(xì)胞數(shù)量增加。
[Abstract]:Objective: to study. In order to improve the lymphatic metastasis model of oral cancer in mice, the difference of oral mucosal lesion in mice induced by tetranitroquinoline 4-nitroquinoline 1-oxidetio (4-NQO) drinking water at different time was compared. There are two pieces of water. The mouse oral cancer model was induced by 4-nitroquinoline-1-oxide-4-NQO (4-nitroquinoline-1-oxide-4-NQO) drinking water. Bone marrow diffusing cells were extracted from different stages of oral mucosa carcinogenesis. The difference of bone marrow spreading cells in different stages of oral mucosa carcinogenesis was compared. Methods one hundred and seventy balb/c mice were randomly divided into two groups: the experimental group (n = 60) was fed with 200mg/L4NQ0 for 14 weeks (20 mice in each group) and the control group (n = 10) was fed with tap water for 45 weeks, and the control group (n = 10). After drinking tap water for 45 weeks, the pathological changes of each group were observed by naked eye and histology, and the changes of body weight of mice were compared. There are two pieces of water. In the first part, the animal model of oral carcinogenesis in mice was established by perfecting the animal model. Ten mice with normal tongue mucosa and 10 mice with simple hyperplasia of tongue were selected by pathological examination. There were 20 mice with mild to moderate dysplasia of tongue, 20 mice with severe dysplasia of tongue and 20 mice with highly differentiated squamous cell carcinoma of tongue. Bone marrow of femur of hind leg of all mice was collected. Mononuclear cells were extracted by Ficoll density gradient centrifugation and made into cell smears. Bone marrow disseminated epithelial cells were identified by immunocytochemistry SP method with PAN-CK epithelial-specific antibody as marker. To compare the number of bone marrow diffusing cells in different periods. The result is that one is one. With the prolongation of administration time, white plaques, ulcers, papillary hyperplasia and other changes appeared in the dorsal tongue mucosa of mice in the experimental group. The typical pathological changes of mouse tongue were mild dysplasia, moderate dysplasia, severe dysplasia, carcinoma in situ and squamous cell carcinoma. In the 8-week group, 70.6% of the mice showed simple hyperplasia of the tongue, 29.4% of the mice showed mild and severe dysplasia, and the incidence of tongue cancer was 0, 88.2% of the 14-week group showed mild and severe dysplasia in situ, and 100% of the 20 weeks of administration showed squamous cell carcinoma. The weight of the mice in the distant metastasis group was significantly lower than that in the control group and the control group at the 8th week after treatment, and there was no significant change in the body weight of the mice in the group of distant metastasis and in the control group compared with the control group before and after administration of the drug, and no significant change was found in the weight of the mice in the group of distant metastasis. Diffusion positive cells were not found in bone marrow cell smears of 10 cases of normal tongue mucosa and 10 cases of simple hyperplasia and 20 cases of mild to moderate dysplasia. Of the 20 mice with severe dysplasia of tongue, 8 cases were diffused positive cells in bone marrow smears, and 13 cases (65%) were positive cells in bone marrow smears of 20 cases of squamous cell carcinoma (SCC). There was a significant difference in the positive rate of disseminated cells between the two groups in severe dysplasia and squamous cell carcinoma group (P 0.05). It was also found that the number of disseminated cells in bone marrow increased with the gradual malignant change of tongue mucosa between the positive group of bone marrow diffusing cells. Conclusion: 1: 1. At the same dose, the oral precancerous lesion and oral cancer rate increased with the prolongation of administration time, but the weight loss was more obvious. There are two pieces of water. With the gradual malignant change of the mouse tongue mucosa, the bone marrow spreading cells appeared in the severe dysplasia of the tongue, and with the severity of the lesion, the incidence of bone marrow spreading cells and the number of bone dissemination cells increased.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R-332;R739.8

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