本文選題：人載脂蛋A-I(ApoA-I) + THP-1細胞��； 參考：《復旦大學》2009年博士論文

【摘要】： 盡管高密度脂蛋白(HDL)的抗炎作用已被廣泛接受,但載脂蛋白A-I(ApoA-I)的抗炎作用卻了解甚少。本論文在以往研究的基礎(chǔ)上,更深入地探討了ApoA-I的抗炎機制,特別是ApoA-I對于單核細胞與內(nèi)皮細胞的相互作用、對內(nèi)毒素(LPS)誘導全身性炎癥兔血漿HDL炎癥指數(shù)的影響。結(jié)果顯示,ApoA-I能顯著減少LPS誘導的THP-1細胞釋放單核細胞趨化因子-1(MCP-1)、降低oxLDL誘導的THP-1細胞遷移率(均為P＜0.01);ApoA-I顯著降低LPS誘導的THP-1細胞釋放可溶性白細胞選擇素(sL-Selectin)、可溶性細胞間粘附分子-1(sICAM-1)、可溶性血管細胞粘附分子-1(sVCAM-1)(分別為P＜0.01,P＜0.01,P＜0.05);ApoA-I顯著抑制LPS誘導的THP-1細胞表面CD11b和VCAM-1的表達(分別為P＜0.01,P＜0.05);ApoA-I能減少LPS誘導的THP-1細胞膜CD14(mCD14)的表達(P＜0.01),抑制NFκB的核內(nèi)轉(zhuǎn)位;單次注射ApoA-I能降低LPS誘導的全身性炎癥兔血漿HDL的炎癥指數(shù),改善HDL的抗炎功能。 結(jié)論:ApoA-I能抑制THP-1細胞趨化、粘附、激活,改善血漿HDL炎癥指數(shù),發(fā)揮抗炎作用。
[Abstract]:Although the anti-inflammatory effects of HDL) have been widely accepted, little is known about the anti-inflammatory effects of apolipoprotein A-Ig ApoA-I. On the basis of previous studies, the mechanism of anti-inflammation of ApoA-I, especially the effect of ApoA-I on the interaction between monocytes and endothelial cells, and on the plasma HDL inflammation index induced by endotoxin in rabbits were discussed. The results showed that ApoA-I could significantly reduce the release of monocyte chemokine, MCP-1, and the mobility of THP-1 cells induced by oxLDL (all P < 0.01). ApoA-I could significantly reduce the release of soluble leukocyte selectin (SL-Selectinine), soluble leukocyte selectin (SL-Selectinine) from THP-1 cells induced by LPS. The expression of CD11b and VCAM-1 on the surface of THP-1 cells induced by LPS (P < 0.01, P < 0.01, P < 0.05, respectively) was significantly inhibited by the intercellular adhesion molecule-1, soluble vascular cell adhesion molecule-1, and soluble vascular cell adhesion molecule-1 (P < 0.01, P < 0.01, respectively), and the nuclear translocation of NF 魏 B was inhibited by ApoA-I (P < 0.01, P < 0.05, respectively), while the expression of CD14mCD14 on THP-1 cell membrane induced by LPS was decreased (P < 0.01), and the nuclear translocation of NF 魏 B was inhibited by ApoA-I (P < 0.01, P < 0.01, respectively). Single injection of ApoA-I could decrease the inflammatory index of plasma HDL and improve the anti-inflammatory function of HDL in rabbits with systemic inflammation induced by LPS. Conclusion: ApoA-I can inhibit the chemotaxis, adhesion and activation of THP-1 cells, improve the inflammatory index of plasma HDL, and play an anti-inflammatory effect.
【學位授予單位】：復旦大學
【學位級別】：博士
【學位授予年份】：2009
【分類號】：R363

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