本文選題：人載脂蛋A-I(ApoA-I) + THP-1細(xì)胞��； 參考：《復(fù)旦大學(xué)》2009年博士論文

【摘要】： 盡管高密度脂蛋白(HDL)的抗炎作用已被廣泛接受,但載脂蛋白A-I(ApoA-I)的抗炎作用卻了解甚少。本論文在以往研究的基礎(chǔ)上,更深入地探討了ApoA-I的抗炎機(jī)制,特別是ApoA-I對(duì)于單核細(xì)胞與內(nèi)皮細(xì)胞的相互作用、對(duì)內(nèi)毒素(LPS)誘導(dǎo)全身性炎癥兔血漿HDL炎癥指數(shù)的影響。結(jié)果顯示,ApoA-I能顯著減少LPS誘導(dǎo)的THP-1細(xì)胞釋放單核細(xì)胞趨化因子-1(MCP-1)、降低oxLDL誘導(dǎo)的THP-1細(xì)胞遷移率(均為P＜0.01);ApoA-I顯著降低LPS誘導(dǎo)的THP-1細(xì)胞釋放可溶性白細(xì)胞選擇素(sL-Selectin)、可溶性細(xì)胞間粘附分子-1(sICAM-1)、可溶性血管細(xì)胞粘附分子-1(sVCAM-1)(分別為P＜0.01,P＜0.01,P＜0.05);ApoA-I顯著抑制LPS誘導(dǎo)的THP-1細(xì)胞表面CD11b和VCAM-1的表達(dá)(分別為P＜0.01,P＜0.05);ApoA-I能減少LPS誘導(dǎo)的THP-1細(xì)胞膜CD14(mCD14)的表達(dá)(P＜0.01),抑制NFκB的核內(nèi)轉(zhuǎn)位;單次注射ApoA-I能降低LPS誘導(dǎo)的全身性炎癥兔血漿HDL的炎癥指數(shù),改善HDL的抗炎功能。 結(jié)論:ApoA-I能抑制THP-1細(xì)胞趨化、粘附、激活,改善血漿HDL炎癥指數(shù),發(fā)揮抗炎作用。
[Abstract]:Although the anti-inflammatory effects of HDL) have been widely accepted, little is known about the anti-inflammatory effects of apolipoprotein A-Ig ApoA-I. On the basis of previous studies, the mechanism of anti-inflammation of ApoA-I, especially the effect of ApoA-I on the interaction between monocytes and endothelial cells, and on the plasma HDL inflammation index induced by endotoxin in rabbits were discussed. The results showed that ApoA-I could significantly reduce the release of monocyte chemokine, MCP-1, and the mobility of THP-1 cells induced by oxLDL (all P < 0.01). ApoA-I could significantly reduce the release of soluble leukocyte selectin (SL-Selectinine), soluble leukocyte selectin (SL-Selectinine) from THP-1 cells induced by LPS. The expression of CD11b and VCAM-1 on the surface of THP-1 cells induced by LPS (P < 0.01, P < 0.01, P < 0.05, respectively) was significantly inhibited by the intercellular adhesion molecule-1, soluble vascular cell adhesion molecule-1, and soluble vascular cell adhesion molecule-1 (P < 0.01, P < 0.01, respectively), and the nuclear translocation of NF 魏 B was inhibited by ApoA-I (P < 0.01, P < 0.05, respectively), while the expression of CD14mCD14 on THP-1 cell membrane induced by LPS was decreased (P < 0.01), and the nuclear translocation of NF 魏 B was inhibited by ApoA-I (P < 0.01, P < 0.01, respectively). Single injection of ApoA-I could decrease the inflammatory index of plasma HDL and improve the anti-inflammatory function of HDL in rabbits with systemic inflammation induced by LPS. Conclusion: ApoA-I can inhibit the chemotaxis, adhesion and activation of THP-1 cells, improve the inflammatory index of plasma HDL, and play an anti-inflammatory effect.
【學(xué)位授予單位】：復(fù)旦大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2009
【分類號(hào)】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 謝金文;萬勇;余為一;;CD14分子的研究進(jìn)展[J];安徽農(nóng)學(xué)通報(bào);2006年04期

2 余燈廣,高秋華,胡錦東,陳然;TBARS法測(cè)定胰島素口腔噴霧劑中脂質(zhì)過氧化物研究[J];分析科學(xué)學(xué)報(bào);2005年01期

3 周興建;蔣綠芝;;MCP-1與胰島素抵抗和2型糖尿病的關(guān)系[J];國際內(nèi)分泌代謝雜志;2006年02期

4 劉興,馮文莉,康格非;脂多糖受體的研究進(jìn)展[J];國外醫(yī)學(xué).臨床生物化學(xué)與檢驗(yàn)學(xué)分冊(cè);2001年03期

5 陳理華;CD14分子的受體作用及其信號(hào)傳遞[J];國外醫(yī)學(xué)(免疫學(xué)分冊(cè));2000年03期

6 彭世國,胡定遠(yuǎn),余建華,李萍;沉淀劑濃度對(duì)測(cè)定高密度脂蛋白膽固醇的影響[J];華西醫(yī)學(xué);1999年04期

7 劉軒,魏育林;LPS受體CD14的最新研究進(jìn)展[J];解剖科學(xué)進(jìn)展;1999年02期

8 徐也魯;動(dòng)脈粥樣硬化——一種慢性炎癥過程[J];中國動(dòng)脈硬化雜志;2001年02期

9 張慧平,孫福成,王抒;高密度脂蛋白與動(dòng)脈粥樣硬化和冠心病[J];中國動(dòng)脈硬化雜志;2004年06期

10 范樂明;動(dòng)脈粥樣硬化炎癥機(jī)制的再認(rèn)識(shí)[J];中國動(dòng)脈硬化雜志;2005年03期

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