本文選題：超順磁性氧化鐵 + 骨髓間充質(zhì)干細(xì)胞��； 參考：《山西醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 背景：一些研究者利用超順磁性氧化鐵對(duì)骨髓間充質(zhì)干細(xì)胞進(jìn)行標(biāo)記,并利用MRI技術(shù)對(duì)標(biāo)記細(xì)胞肝內(nèi)、腎內(nèi)移植后進(jìn)行初步的活體示蹤,但是,對(duì)于超順磁性氧化鐵標(biāo)記骨髓間充質(zhì)干細(xì)胞后,對(duì)其存活、增殖以及分化是否有影響研究較少。 目的：觀察超順磁性氧化鐵標(biāo)記骨髓間充質(zhì)干細(xì)胞后,對(duì)其存活、增殖以及向神經(jīng)細(xì)胞的分化是否有影響。 方法：使用超順磁性氧化鐵標(biāo)記大鼠骨髓間充質(zhì)干細(xì)胞,采用普魯士藍(lán)染色法鑒定其標(biāo)記率、臺(tái)盤(pán)藍(lán)染色法檢測(cè)細(xì)胞活力、MTT法檢測(cè)標(biāo)記干細(xì)胞的細(xì)胞增值活力、以1 mmol／Lβ-巰基乙醇及無(wú)血清DMEM培養(yǎng)液體外誘導(dǎo)標(biāo)記細(xì)胞向神經(jīng)細(xì)胞分化并用免疫組織化學(xué)鑒定誘導(dǎo)后細(xì)胞、再次使用普魯士藍(lán)染色法鑒定神經(jīng)細(xì)胞內(nèi)的鐵顆粒。 結(jié)果與結(jié)論：普魯士藍(lán)染色對(duì)干細(xì)胞的標(biāo)記率接近100%,臺(tái)盤(pán)藍(lán)染色顯示標(biāo)記細(xì)胞的存活率不受影響；MTT法檢測(cè)發(fā)現(xiàn)標(biāo)記干細(xì)胞的增殖活力與未標(biāo)記干細(xì)胞相比差異無(wú)顯著性意義(P0.05)；以β-巰基乙醇誘導(dǎo)后大部分骨髓間充質(zhì)干細(xì)胞分化為神經(jīng)元樣細(xì)胞、免疫組織化學(xué)陽(yáng)性,再次普魯士藍(lán)染色顯示鐵顆粒位于神經(jīng)細(xì)胞的細(xì)胞漿內(nèi)。提示超順磁性氧化鐵標(biāo)記骨髓間充質(zhì)干細(xì)胞后,對(duì)于干細(xì)胞的存活、增殖以及向神經(jīng)細(xì)胞的分化無(wú)影響。
[Abstract]:Background: some researchers used superparamagnetic ferric oxide to label bone marrow mesenchymal stem cells and MRI technique was used to trace the labeled cells in liver and kidney, but, There are few studies on the survival, proliferation and differentiation of bone marrow mesenchymal stem cells labeled with superparamagnetic iron oxide. Aim: to investigate the effects of superparamagnetic ferric oxide on the survival, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods: superparamagnetic ferric oxide was used to label rat bone marrow mesenchymal stem cells (BMSCs). Prussian blue staining was used to identify the labeling rate, and table-blue staining was used to detect cell viability. MTT assay was used to detect the cell proliferation activity of labeled stem cells. The labeled cells were induced to differentiate into neural cells with 1 mmol/L 尾 -mercaptoethanol and serum-free DMEM in vitro. The induced cells were identified by immunohistochemistry, and the iron granules in the neurons were identified by Prussian blue staining. Results and conclusion: the labeling rate of stem cells by Prussian blue staining was close to 100. The viability of labeled stem cells was not affected by plate blue staining. The proliferative activity of labeled stem cells was not different from that of unlabeled stem cells by MTT assay. Most bone marrow mesenchymal stem cells differentiated into neuron-like cells after induced by 尾 -mercaptoethanol. Immunohistochemical staining showed that iron granules were located in the cytoplasm of nerve cells. These results suggest that superparamagnetic ferric oxide labeled bone marrow mesenchymal stem cells has no effect on the survival, proliferation and differentiation of stem cells.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 方亮;施曉雷;褚薛慧;方勝;丁義濤;;磁標(biāo)記豬骨髓間充質(zhì)干細(xì)胞自體肝內(nèi)移植后MR活體示蹤研究[J];肝膽外科雜志;2008年03期

2 居勝紅,滕皋軍,毛曦,張愛(ài)鳳,張宇,馬明,顧寧;臍血間充質(zhì)干細(xì)胞磁探針標(biāo)記和MR成像研究[J];中華放射學(xué)雜志;2005年01期

3 孫軍輝;滕皋軍;居勝紅;馬占龍;麥筱莉;張宇;馬明;;腎功能衰竭大鼠超順磁性氧化鐵標(biāo)記骨髓間充質(zhì)干細(xì)胞腎臟移植MR活體示蹤的研究[J];中華放射學(xué)雜志;2006年02期

4 ;Functional recovery after rhesus monkey spinal cord injury by transplantation of bone marrow mesenchymal-stem cell-derived neurons[J];Chinese Medical Journal;2005年18期

5 景猛,劉新權(quán),梁鵬,李長(zhǎng)宇,張相彤,王丹,欒鈺,劉恩重;應(yīng)用超順磁性氧化鐵納米粒子標(biāo)記神經(jīng)干細(xì)胞及活體磁共振示蹤的實(shí)驗(yàn)研究[J];中華醫(yī)學(xué)雜志;2004年16期

6 ;Characterization and enrichment of hepatic progenitor cells in adult rat liver[J];World Journal of Gastroenterology;2004年10期

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本文編號(hào)：1832549