發(fā)布時(shí)間：2018-05-01 17:00

  本文選題：軟骨細(xì)胞 + 組織工程��； 參考：《大連醫(yī)科大學(xué)》2010年博士論文

【摘要】： 目的：微流控芯片平臺(tái)上探索三維培養(yǎng)環(huán)境下胰島素樣生長(zhǎng)因子-1(Insulin-Like Growth Factor-1, IGF-1)、堿性成纖維細(xì)胞生長(zhǎng)因子(basic Fibroblast Growth Factor, bFGF)對(duì)軟骨細(xì)胞體外增殖的影響；通過(guò)驗(yàn)證微流控平臺(tái)的有效性將微流控芯片技術(shù)引入軟骨組織工程領(lǐng)域。 方法：微流控芯片上,分別在IGF-1、bFGF以及二者組合濃度梯度下三維培養(yǎng)軟骨細(xì)胞,兩周后計(jì)算軟骨細(xì)胞增殖率并與96孔板傳統(tǒng)方法所獲得的數(shù)據(jù)進(jìn)行比較。 結(jié)果：IGF-1在57.14ng/ml產(chǎn)生最大增殖效應(yīng)(平均倍增2.38倍),bFGF在5.72ng/ml產(chǎn)生最大增殖效應(yīng)(平均倍增3.85倍),85.71ng/ml的IGF-1與1.43ng/ml bFGF的組合使軟骨細(xì)胞產(chǎn)生最大的增殖效應(yīng)(平均倍增4.76倍)；芯片與傳統(tǒng)96孔板所得增殖率不存在統(tǒng)計(jì)學(xué)顯著性差異。 結(jié)論：一定濃度組合的IGF-1和bFGF可協(xié)同產(chǎn)生最大的增殖效應(yīng)；微流控芯片技術(shù)可有效應(yīng)用于軟骨組織工程領(lǐng)域,其在軟骨組織工程領(lǐng)域存在廣闊的應(yīng)用前景。
[Abstract]:Objective: to explore the effects of insulin-like Growth Factor-1 (IGF-1) and basic fibroblast growth factor (basic Fibroblast Growth Factor, bFGF) on the proliferation of chondrocytes in three dimensional culture on microfluidic chip. The microfluidic chip technology is introduced into the cartilage tissue engineering field by verifying the effectiveness of the microfluidic platform. Methods: the chondrocytes were cultured on microfluidic chip under IGF-1bFGF and their combined concentration gradient. The proliferation rate of chondrocytes was calculated two weeks later and compared with the data obtained by the traditional method of 96-well plate. Results the maximum proliferative effect (average doubling 2.38 times) of 57.14ng/ml was produced by the combination of IGF-1 and 1.43ng/ml bFGF (the combination of IGF-1 and 1.43ng/ml bFGF was 3.85 times and 85.71ng / ml), and the proliferation effect of chondrocytes was 4.76 times (average doubling 4.76 times). There was no significant difference in the proliferation rate between the plate and the traditional 96 hole plate. Conclusion: the combination of IGF-1 and bFGF with a certain concentration can produce the greatest proliferative effect and microfluidic chip technology can be effectively applied in cartilage tissue engineering and has a broad application prospect in the field of cartilage tissue engineering.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2010
【分類號(hào)】：R329

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

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