本文選題：流感病毒 + 表位��； 參考：《吉林大學》2008年碩士論文

【摘要】： 本研究根據(jù)當前流感的流行現(xiàn)狀和多個亞型高致病性禽流感病毒跨種傳播的現(xiàn)實,成功應用生物信息學理論篩選出流感病毒的多個優(yōu)勢抗原表位(流感病毒內(nèi)部保守的M、NP蛋白CTL表位,和H1、H7、H9 HA的Th細胞表位),設(shè)計并合成了針對多個亞型流感病毒的通用多表位基因盒Epi I和Epi II。以pVAX1真核表達質(zhì)粒為載體構(gòu)建與篩選出共表達NP與Epi I基因盒的DNA重組體pVAX1-NP-Epi I,共表達H5、H3亞型IV HA與Epi II基因的DNA重組體pVAX1-H5HA-Epi II-H3HA1-M2e。 應用BALB/c小鼠動物模型進行了免疫原性初步研究,免疫結(jié)果顯示重組DNA疫苗誘導機體產(chǎn)生了抗流感H1、H3、H5、H7和H9 HA的特異性體液和細胞免疫,其中誘導的H5HA、H3HA1抗體水平較高;淋巴細胞轉(zhuǎn)化試驗、淋巴細胞亞類FACS檢測和γIFN-ELISPOT細胞免疫指標檢測顯示,多表位重組體均誘導了較強的細胞免疫,說明所構(gòu)建的DNA重組體有良好的免疫原性,為最終獲得能同時預防多種亞型流感病毒的多價疫苗奠定了基礎(chǔ)。
[Abstract]:According to the current epidemic situation of influenza and the interspecies transmission of several subtypes of highly pathogenic avian influenza virus, Several dominant antigenic epitopes of influenza virus (CTL epitopes of MNP protein in influenza virus) were successfully screened by bioinformatics theory. The universal multiepitope gene cassette Epi I and Epi II for multiple subtypes of influenza virus were designed and synthesized. Using pVAX1 eukaryotic expression plasmid as the vector, the DNA recombinant pVAX1-NP-Epi I was constructed and screened, and the DNA recombinant pVAX1-H5HA-Epi II-H3HA1-M2eof H5H3 subtype IV HA and Epi II gene was coexpressed. The immunogenicity of BALB/c mouse model was studied. The results showed that the recombinant DNA vaccine induced specific humoral and cellular immunity against H1H3H3H5H5H7 and H9HA, and the antibody level of H5H3HA1 was higher than that of H9HA. Lymphocyte transformation test, lymphocyte subclass FACS detection and 緯 IFN-ELISPOT cell immune index detection showed that the multiepitope recombinant induced strong cellular immunity, indicating that the constructed DNA recombinant had good immunogenicity. It lays the foundation for obtaining multivalent vaccine which can prevent multiple subtypes of influenza virus at the same time.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R392

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