本文選題：流感病毒 + 表位��； 參考：《吉林大學(xué)》2008年碩士論文

【摘要】： 本研究根據(jù)當(dāng)前流感的流行現(xiàn)狀和多個(gè)亞型高致病性禽流感病毒跨種傳播的現(xiàn)實(shí),成功應(yīng)用生物信息學(xué)理論篩選出流感病毒的多個(gè)優(yōu)勢(shì)抗原表位(流感病毒內(nèi)部保守的M、NP蛋白CTL表位,和H1、H7、H9 HA的Th細(xì)胞表位),設(shè)計(jì)并合成了針對(duì)多個(gè)亞型流感病毒的通用多表位基因盒Epi I和Epi II。以pVAX1真核表達(dá)質(zhì)粒為載體構(gòu)建與篩選出共表達(dá)NP與Epi I基因盒的DNA重組體pVAX1-NP-Epi I,共表達(dá)H5、H3亞型IV HA與Epi II基因的DNA重組體pVAX1-H5HA-Epi II-H3HA1-M2e。 應(yīng)用BALB/c小鼠動(dòng)物模型進(jìn)行了免疫原性初步研究,免疫結(jié)果顯示重組DNA疫苗誘導(dǎo)機(jī)體產(chǎn)生了抗流感H1、H3、H5、H7和H9 HA的特異性體液和細(xì)胞免疫,其中誘導(dǎo)的H5HA、H3HA1抗體水平較高;淋巴細(xì)胞轉(zhuǎn)化試驗(yàn)、淋巴細(xì)胞亞類FACS檢測(cè)和γIFN-ELISPOT細(xì)胞免疫指標(biāo)檢測(cè)顯示,多表位重組體均誘導(dǎo)了較強(qiáng)的細(xì)胞免疫,說(shuō)明所構(gòu)建的DNA重組體有良好的免疫原性,為最終獲得能同時(shí)預(yù)防多種亞型流感病毒的多價(jià)疫苗奠定了基礎(chǔ)。
[Abstract]:According to the current epidemic situation of influenza and the interspecies transmission of several subtypes of highly pathogenic avian influenza virus, Several dominant antigenic epitopes of influenza virus (CTL epitopes of MNP protein in influenza virus) were successfully screened by bioinformatics theory. The universal multiepitope gene cassette Epi I and Epi II for multiple subtypes of influenza virus were designed and synthesized. Using pVAX1 eukaryotic expression plasmid as the vector, the DNA recombinant pVAX1-NP-Epi I was constructed and screened, and the DNA recombinant pVAX1-H5HA-Epi II-H3HA1-M2eof H5H3 subtype IV HA and Epi II gene was coexpressed. The immunogenicity of BALB/c mouse model was studied. The results showed that the recombinant DNA vaccine induced specific humoral and cellular immunity against H1H3H3H5H5H7 and H9HA, and the antibody level of H5H3HA1 was higher than that of H9HA. Lymphocyte transformation test, lymphocyte subclass FACS detection and 緯 IFN-ELISPOT cell immune index detection showed that the multiepitope recombinant induced strong cellular immunity, indicating that the constructed DNA recombinant had good immunogenicity. It lays the foundation for obtaining multivalent vaccine which can prevent multiple subtypes of influenza virus at the same time.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R392

【參考文獻(xiàn)】

相關(guān)期刊論文 前9條

1 郭元吉;幾種正在研究中的流感疫苗[J];國(guó)外醫(yī)學(xué).病毒學(xué)分冊(cè);1994年04期

2 彭金美,童光志,王云峰,仇華吉;抗禽流感病毒多表位DNA疫苗的構(gòu)建及其免疫效力研究[J];生物工程學(xué)報(bào);2003年05期

3 程堅(jiān),劉秀梵,彭大新,劉紅旗;表達(dá)H9亞型禽流感病毒血凝素基因的重組雞痘病毒及其免疫效力[J];微生物學(xué)報(bào);2002年04期

4 羅開(kāi)健,辛朝安,薛義山,楊克軍,黎崇洪;禽流感二價(jià)抗原油乳劑滅活疫苗的研制[J];中國(guó)獸醫(yī)科技;2000年07期

5 唐秀英,田國(guó)斌,于康震,馮菊艷;禽流感油乳劑滅活疫苗的研究[J];中國(guó)預(yù)防獸醫(yī)學(xué)報(bào);1999年06期

6 李子健,金寧一,江文正,張立樹(shù);HIV復(fù)合多表位DNA疫苗的設(shè)計(jì)及免疫研究[J];中華微生物學(xué)和免疫學(xué)雜志;2004年11期

7 戚中田;禽流感病毒與人類禽流感[J];中國(guó)科學(xué)基金;2004年02期

8 陳化蘭,于康震,田國(guó)濱,唐秀英,盧景良;DNA免疫誘導(dǎo)雞對(duì)禽流感病毒的免疫保護(hù)反應(yīng)[J];中國(guó)農(nóng)業(yè)科學(xué);1998年05期

9 陳吉祥,李廣林,薛飛群,陳化蘭,于康震,趙榮材,李樹(shù)本;禽流感HA基因疫苗脂質(zhì)體的制備及其理化性質(zhì)[J];中國(guó)獸醫(yī)學(xué)報(bào);1999年03期

，

本文編號(hào)：1801294