本文選題：流感病毒 + 細(xì)胞凋亡��； 參考：《山東師范大學(xué)》2008年碩士論文

【摘要】： (一)細(xì)胞凋亡是一個非常重要的細(xì)胞生理過程,它對于維持機(jī)體自身的穩(wěn)定性和個體正常的生長發(fā)育以及抑制腫瘤發(fā)生等許多方面都起著重要的作用。目前研究表明,許多病毒的感染都能夠誘導(dǎo)宿主細(xì)胞的凋亡,但其具體的機(jī)制還不清楚,包括是病毒的什么成份引起了宿主細(xì)胞的凋亡以及宿主細(xì)胞是通過什么途徑走向凋亡的。 凋亡誘導(dǎo)因子(apoptosis-inducing factor, AIF)和線粒體內(nèi)切核酸酶G(endonuclease G, Endo G)在細(xì)胞凋亡中起到了非常重要的作用,但其發(fā)揮凋亡效應(yīng)的特點(diǎn)還沒有定論,在不同的凋亡誘導(dǎo)因素下可能會有不同的行為特點(diǎn)。本文首先證明了AIF和Endo G在流感病毒感染誘導(dǎo)細(xì)胞凋亡過程中起到了重要的作用,重點(diǎn)研究了AIF和Endo G發(fā)揮的凋亡誘導(dǎo)效應(yīng)與caspase活化之間的關(guān)系,發(fā)現(xiàn)二者有著不同的作用特點(diǎn)。本文還研究了AIF和Endo G在誘導(dǎo)細(xì)胞凋亡過程中與P53活化以及Hsp70等相關(guān)蛋白的關(guān)系。 (二)蛋白質(zhì)的泛素化修飾是蛋白質(zhì)的一種重要的翻譯后水平修飾,人們對泛素-蛋白酶體途徑介導(dǎo)的蛋白質(zhì)的降解機(jī)制也已經(jīng)有了較深入地了解,但近年來發(fā)現(xiàn)有些蛋白質(zhì)(例如組蛋白)在被泛素化修飾后并不能使蛋白質(zhì)降解,而是對蛋白質(zhì)的功能進(jìn)行調(diào)控。 Dhr6是酵母中Rad6的在果蠅中的同源蛋白,在酵母中Rad6是催化泛素化過程中起E2作用的酶,并且在DNA損傷修復(fù)中發(fā)揮著重要的作用。但對果蠅Dhr6的研究就尚未見報道,本文重點(diǎn)研究了果蠅Dhr6的亞細(xì)胞定位以及對DNA損傷試劑MMS的應(yīng)答特點(diǎn),還研究了另外一個同樣處于E2位值的泛素化酶DubcX與Dhr6的行為差別,最后優(yōu)化了Dhr6的RNAi實(shí)驗(yàn)條件。
[Abstract]:(1) apoptosis is a very important cellular physiological process, which plays an important role in maintaining the stability of the body itself, normal growth and development of the individual, and inhibiting tumorigenesis. Current studies have shown that many viral infections can induce apoptosis in host cells, but its specific mechanism is not clear. Including what components of the virus cause the apoptosis of host cells and through what way the host cells apoptosis. Apoptosis-inducing factors (AIFs) and mitochondrial endonuclease G(endonuclease G (Endo G) play a very important role in apoptosis, but the characteristics of apoptosis-inducing factor (AIFs) and mitochondrial endonuclease G(endonuclease G (Endo G) play an important role in apoptosis, but the characteristics of apoptosis-inducing factor play an important role in apoptosis. There may be different behavioral characteristics under different apoptosis-inducing factors. In this paper, we first demonstrated that AIF and Endo G play an important role in the process of apoptosis induced by influenza virus infection, and focused on the relationship between the apoptosis-inducing effect of AIF and Endo G and the activation of caspase. It is found that the two have different characteristics. We also studied the relationship between AIF and Endo G and p53 activation, Hsp70 and other related proteins in the process of inducing apoptosis. (2) the ubiquification modification of proteins is an important post-translational modification of proteins, and the mechanism of protein degradation mediated by the ubiquitin proteasome pathway has been well understood. However, in recent years, it has been found that some proteins (such as histone) can not degrade proteins after being modified by ubiquitin, but regulate the function of proteins. Dhr6 is the homologous protein of Rad6 in Drosophila and Rad6 is the enzyme that catalyzes ubiquification and plays an important role in the repair of DNA damage. However, the study of Drosophila Dhr6 has not been reported. In this paper, the subcellular localization of Drosophila Dhr6 and its response to DNA damage reagent MMS were studied, and the behavioral differences between DubcX and Dhr6, another ubiquitin enzyme at E2 level, were also studied. Finally, the RNAi experimental conditions of Dhr6 are optimized.
【學(xué)位授予單位】：山東師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R373

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