本文選題：瑞士乳桿菌 + 腸道微生物區(qū)系　； 參考：《天津商業(yè)大學(xué)》2008年碩士論文

【摘要】： 益生菌作為一種活的有機(jī)體,存在于腸道粘膜上,并對(duì)腸道黏膜有保護(hù)和調(diào)節(jié)作用,可以治療各種腸道疾病。益生菌可直接作用于宿主的免疫系統(tǒng),誘發(fā)腸道免疫,并刺激胸腺、脾臟和法氏囊等免疫器官的發(fā)育,促進(jìn)巨噬細(xì)胞活力或發(fā)揮佐劑作用,活化腸粘膜內(nèi)相關(guān)淋巴組織,使SIgA分泌增加。益生菌在體液免疫和細(xì)胞免疫中發(fā)揮著調(diào)節(jié)作用,其保健作用的機(jī)理成為近年來(lái)研究的重點(diǎn)內(nèi)容。本文選用瑞士乳桿菌(Lactobacillus helveticusTS206)作為研究菌株,系統(tǒng)地研究了其對(duì)小鼠腸道粘膜免疫的影響。本研究首先采用了改良培養(yǎng)基的方法,選擇了瑞士乳桿菌的最適培養(yǎng)基;接著利用蛋白質(zhì)的溶解特性和梯度離心的方法建立了菌體細(xì)胞分離純化技術(shù);然后采用選擇性培養(yǎng)技術(shù)系統(tǒng)研究了小鼠腸道微生物區(qū)系;用放射性免疫(RIA)和酶聯(lián)免疫(ELISA)檢測(cè)技術(shù)系統(tǒng)評(píng)價(jià)了小鼠腸道粘膜中SIgA和腸道粘膜中的分泌細(xì)胞因子;同時(shí)觀察條件致病菌(E.coli)灌胃組對(duì)小鼠粘膜免疫的影響,研究瑞士乳桿菌對(duì)小鼠腸粘膜免疫系統(tǒng)的作用方式。 實(shí)驗(yàn)結(jié)果表明:①瑞士乳桿菌的最適培養(yǎng)基為溴甲酚綠指示劑培養(yǎng)基。②瑞士乳桿菌對(duì)小鼠腸道微生物區(qū)系調(diào)節(jié)的最佳灌胃劑量為2×10~7cfu/d。③長(zhǎng)期灌胃瑞士乳桿菌對(duì)小鼠腸道粘膜中SIgA含量的影響:瑞士乳桿菌灌胃組小鼠腸道粘膜中SIgA的含量在第10天達(dá)到最大值,和對(duì)照組相比差異極顯著(P＜0.01),腸道粘膜中SIgA含量顯著升高。短期灌胃瑞士乳桿菌對(duì)小鼠腸道粘膜中SIgA含量的影響:瑞士乳桿菌灌胃組小鼠腸道粘膜中SIgA含量在24h內(nèi)持續(xù)上升。④長(zhǎng)期灌胃瑞士乳桿菌對(duì)小鼠腸道中細(xì)胞因子的影響:瑞士乳桿菌灌胃組小鼠腸道中四種細(xì)胞因子和比對(duì)照組明顯升高(P＜0.05),瑞士乳桿菌灌胃組小鼠腸道內(nèi)Th1型細(xì)胞因子(IL-2,IFN-γ)在第3d前達(dá)到最大值;Th2型細(xì)胞因子(IL-4,IL-6)在第5d達(dá)到頂峰;Th1型和Th2型T細(xì)胞因子的比例和對(duì)照組相比無(wú)顯著性差異(P＞0.05),腸道免疫功能未出現(xiàn)異常;大腸桿菌灌胃組則出現(xiàn)了異常。大腸桿菌灌胃組則出現(xiàn)異常。短期灌胃瑞士乳桿菌對(duì)小鼠腸道中細(xì)胞因子的影響:IL-2和IL-6在第6h達(dá)到最高點(diǎn);IL-4在第1h達(dá)到最高點(diǎn);IFN-γ在第3h達(dá)到最高值;Th2型細(xì)胞因子比Th1型的多,于是可以推測(cè)出Th1型T細(xì)胞可能是比Th2型的少一些,這是由于T細(xì)胞短時(shí)間內(nèi)不同分化方向造成的。和長(zhǎng)期灌胃組相比短期灌胃組細(xì)胞因子的波動(dòng)比較大,而長(zhǎng)期灌胃組的結(jié)果更能反映瑞士乳桿菌對(duì)小鼠腸道粘膜免疫的調(diào)理作用。
[Abstract]:Probiotics, as a living organism, exist on the intestinal mucosa and can protect and regulate the intestinal mucosa, which can treat various intestinal diseases. Probiotics can directly act on the host's immune system, induce intestinal immunity, stimulate the development of immune organs such as thymus, spleen and bursa of Fabricius, promote the activity of macrophages or play an adjuvant role, and activate the lymphoid tissues associated with intestinal mucosa. Increase the secretion of SIgA. Probiotics play a regulatory role in humoral and cellular immunity. The effects of Lactobacillus helveticus TS206 on intestinal mucosal immunity in mice were studied systematically. In this study, the method of improved culture medium was used to select the optimum medium for Lactobacillus helicus, and then the isolation and purification technology of bacteria somatic cells was established by using protein dissolution characteristics and gradient centrifugation. The intestinal microflora of mice was studied by selective culture technique, and the SIgA and secreted cytokines in intestinal mucosa of mice were evaluated by radioimmunoassay (RIA) and enzyme linked immunosorbent assay (Elisa). At the same time, the effects of E. coli on mucosal immunity in mice were observed, and the effects of Lactobacillus helicum on intestinal mucosal immune system in mice were studied. The results showed that the optimal medium for Lactobacillus Switzerland was bromocresol green indicator medium. 2 Lactobacillus helittidis for the regulation of intestinal microflora in mice. 2 脳 10~7cfu/d.3 for a long period of time. Effect of SIgA content in intestinal mucosa of mice: the content of SIgA in intestinal mucosa of Lactobacillus helicum group reached its maximum on the 10th day. Compared with the control group, the difference was very significant (P < 0.01), and the content of SIgA in intestinal mucosa was significantly higher than that in the control group (P < 0.01). Effect of Lactobacillus helicum on SIgA content in intestinal mucosa of mice: the content of SIgA in intestinal mucosa of Lactobacillus Switzerland group increased continuously within 24 hours. 4. Long term administration of Lactobacillus helicus on cytokines in intestinal tract of mice The effects of lactobacillus helicobacterium on the four cytokines in the intestinal tract of mice were significantly higher than those in the control group (P < 0.05), and the Th1 type cytokine (IL-2IFN- 緯) in the intestinal tract of the Lactobacillus hominis group reached the maximum value before the third day, and the level of IL-4 / IL-6 of Th2 cytokines was significantly higher than that of the control group (P < 0.05). On the 5th day, there was no significant difference in the proportion of T cytokines of Th1 type and Th2 type between the control group and the control group (P > 0.05), and there was no abnormal intestinal immune function. The group of Escherichia coli was abnormal. The group of Escherichia coli was abnormal. Effects of short term administration of Lactobacillus helicum on cytokines in the intestinal tract of mice, the highest levels of IL 4 and IL 2 were reached at 6 h and the highest level of IFN- 緯 at 1 h. Th2 type cytokines were more than those of Th1 type at 3 h. It can be inferred that Th1 type T cells may be less than those of Th2 type, which is due to the different differentiation directions of T cells in a short period of time. Compared with the long-term group, the fluctuation of cytokines in the short-term group was larger than that in the long-term group, and the results of the long-term group could reflect the regulation effect of Lactobacillus helicum on the intestinal mucosal immunity of mice.
【學(xué)位授予單位】：天津商業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R371

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 劉海燕;轉(zhuǎn)nisI基因植物乳桿菌Lactobacillus plantarum 590對(duì)SD大鼠腸道健康的影響研究[D];江南大學(xué);2011年

相關(guān)碩士學(xué)位論文 前1條

1 李美君;乳鐵蛋白對(duì)早期斷奶仔豬腸道結(jié)構(gòu)和免疫功能的影響研究[D];湖南農(nóng)業(yè)大學(xué);2011年

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本文編號(hào)：1774302