發(fā)布時間：2018-04-16 07:52

  本文選題：降鈣素基因相關(guān)肽 + 骨重建��； 參考：《吉林大學(xué)》2010年博士論文

【摘要】： 骨組織存在神經(jīng)分布目前已被證實(shí),但骨組織內(nèi)神經(jīng)的功能研究尚處于初級階段。各種對骨的生理、病理過程的論述中也極少提及神經(jīng)系統(tǒng)的作用。但臨床上觀察到的一些現(xiàn)象,如Charcot關(guān)節(jié)、異位骨化、假體松動、骨質(zhì)疏松、肥胖相關(guān)的骨密度增加,都被發(fā)現(xiàn)有神經(jīng)系統(tǒng)參與的跡象。我們也觀察到患有神經(jīng)纖維瘤病的患者有的表現(xiàn)為骨形成過度,如巨肢癥,而有的表現(xiàn)為骨形成不良,如先天性脛骨假關(guān)節(jié)。這說明不同的神經(jīng)瘤類型可能是導(dǎo)致患者不同表型的原因。所有這些都提示我們骨骼與神經(jīng)系統(tǒng)存在內(nèi)在聯(lián)系。骨重建是骨骼的最重要的生理過程,骨重建過程是上述病理生理過程最終導(dǎo)致骨質(zhì)結(jié)構(gòu)改變的“執(zhí)行者”。因此,研究神經(jīng)系統(tǒng)在骨組織中的作用自然要著重研究其對骨重建過程的影響。這就是我們立題的出發(fā)點(diǎn)。降鈣素基因相關(guān)肽(CGRP)是目前已知與成骨關(guān)系最密切、在骨組織中分布最為廣泛的一種神經(jīng)肽。在體內(nèi),CGRP對骨重建的影響,目前存在兩種不同的看法,一種是CGRP促進(jìn)骨形成,另一種看法認(rèn)為在體內(nèi)CGRP不具有促進(jìn)骨形成作用,雙方各有各自的實(shí)驗證據(jù)。本研究通過在兔脛骨髓腔內(nèi)植入形狀記憶合金條,從內(nèi)部對兔脛骨施加一個彎曲應(yīng)力,建立了一個彎曲應(yīng)力刺激下的骨重建模型,同時考察了骨重建與CGRP在骨重建局部表達(dá)的相互關(guān)系,發(fā)現(xiàn)在骨內(nèi)CGRP表達(dá)與骨重建呈顯著正相關(guān),說明CGRP免疫陽性神經(jīng)纖維參與體內(nèi)骨重建過程,進(jìn)而也說明神經(jīng)系統(tǒng)對骨重建存在調(diào)控作用。本研究創(chuàng)新點(diǎn):①成功建立了兔脛骨在長期彎曲應(yīng)力刺激下的骨干皮質(zhì)骨骨重建模型,證實(shí)骨重建過程中骨形成的量與所承受的應(yīng)力呈正相關(guān)。②本研究證實(shí)在兔脛骨皮質(zhì)骨重建過程中CGRP促進(jìn)骨形成。③本研究提示神經(jīng)系統(tǒng)參與骨重建調(diào)節(jié),CGRP免疫陽性神經(jīng)纖維是神經(jīng)系統(tǒng)調(diào)控骨重建過程的重要媒介之一。
[Abstract]:The distribution of nerve in bone tissue has been confirmed, but the function of nerve in bone tissue is still in the primary stage.The role of the nervous system is rarely mentioned in the discussion of the physiological and pathological processes of bone.But some clinical phenomena, such as Charcot joint, ectopic ossification, loosening of prosthesis, osteoporosis, increased bone mineral density associated with obesity, have been found to be involved in the nervous system.We also observed that some patients with neurofibromatosis presented with hyperosteogenesis, such as macrosomia, and others with poor bone formation, such as congenital pseudarthrosis of the tibia.This suggests that different types of neuroma may be responsible for different phenotypes of patients.All this suggests that our bones are intrinsically linked to the nervous system.Bone reconstruction is the most important physiological process of bone.Therefore, the study of the role of nervous system in bone tissue should focus on its impact on bone reconstruction process.This is the starting point of our problem.Calcitonin gene-related peptide (CGRP) is known to be the most closely related to osteogenesis and most widely distributed in bone tissue.There are two different views on the effect of CGRP on bone reconstruction in vivo, one is that CGRP promotes bone formation, the other is that CGRP does not promote bone formation in vivo, and each side has its own experimental evidence.In this study, a bone reconstruction model was established by inserting a shape memory alloy strip into the rabbit tibial medullary cavity and applying a bending stress to the rabbit tibia from the inside.At the same time, the relationship between bone reconstruction and local expression of CGRP in bone reconstruction was investigated. It was found that the expression of CGRP in bone was positively correlated with bone reconstruction, indicating that CGRP immunoreactive nerve fibers were involved in the process of bone reconstruction in vivo.It also shows that the nervous system has a regulatory effect on bone reconstruction.In this study, the model of cortical bone reconstruction of the rabbit tibia under long-term bending stress was successfully established by the innovative point: 1.This study confirms that CGRP promotes bone formation in rabbit tibia cortical bone reconstruction. 3. This study suggests that the nervous system is involved in the regulation of bone remodeling. 2. This study suggests that the nervous system participates in the regulation of bone remodeling.The positive nerve fiber is one of the important mediums of nervous system regulating bone reconstruction.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2010
【分類號】：R392.1

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本文編號：1757940