本文選題：大鼠 + 煤工塵肺��； 參考：《吉林大學》2009年博士論文

【摘要】： 本研究目的旨在建立一種更為合適的安全性高、重復(fù)性好、準確、經(jīng)濟、易于制作和評價的煤工塵肺大鼠動物模型。采用氣溶膠吸入法和非暴露氣管灌注法2種染塵方式建立大鼠煤工塵肺動物模型,分別于染塵后2周、4周、8周、3個月、6個月、9個月、12個月、18個月檢測各組實驗動物肺功能、血氣分析、支氣管肺泡灌洗液細胞成分、肺泡灌洗液中TNF-α及TGF-β水平、支氣管肺泡灌洗液細胞TNF-α及TGF-β的mRNA表達水平、肺泡巨噬細胞非特異性吞噬功能、肺組織病理、肺組織內(nèi)羥脯氨酸的含量等指標;并比較各組所制動物模型不同時間、各項指標變化趨勢及相關(guān)性。通過此項研究,我們首先建立了煤塵氣溶膠吸入裝置及煤塵氣溶膠的制備,并成功采用非暴露氣管灌注法和氣溶膠吸入法2種染塵方式建立大了鼠煤工塵肺動物模型,兩種方法均準確、經(jīng)濟地復(fù)制了大鼠煤工塵肺動物模型,以高濃度暴露更為理想;但非氣管暴露灌注法對試驗者實驗技術(shù)復(fù)雜、動物死亡率較高、短時間內(nèi)可復(fù)制動物模型;氣溶膠吸入法動物死亡率低、符合煤工塵肺致病因素和發(fā)病環(huán)境及煤工塵肺的發(fā)生發(fā)展過程,簡便、易于制作和評價,更為適用于煤工塵肺發(fā)病機制、診斷及治療的臨床研究。
[Abstract]:The purpose of this study was to establish a more suitable animal model of coal worker pneumoconiosis with high safety, good reproducibility, accuracy, economy and easy to be made and evaluated.The animal models of coal worker pneumoconiosis were established by aerosol inhalation and non-exposed tracheal instillation. The lung function of experimental animals in each group was measured at 4 weeks, 8 weeks, 3 months, 6 months, 9 months, 12 months and 18 months after dust exposure, respectively.Blood gas analysis, cell composition of bronchoalveolar lavage fluid, TNF- 偽 and TGF- 尾 levels in bronchoalveolar lavage fluid, mRNA expression level of TNF- 偽 and TGF- 尾 in bronchoalveolar lavage fluid cells, nonspecific phagocytosis of alveolar macrophages, lung histopathology.The content of hydroxyproline in lung tissue was compared and the change trend and correlation of each index were compared.Through this study, we first established the coal dust aerosol inhalation device and the preparation of coal dust aerosol, and successfully established the animal model of coal worker pneumoconiosis by two kinds of dusting methods: unexposed trachea perfusion method and aerosol inhalation method.The two methods were both accurate and economically duplicated the animal model of coal worker pneumoconiosis in rats, but the non-tracheal exposure perfusion method was more suitable for the experimental technique of the experimenter, the mortality rate of the animal was higher, and the animal model could be reproduced in a short time.The aerosol inhalation method is suitable for the clinical study of pathogenesis, diagnosis and treatment of coal pneumoconiosis, because of its low mortality rate, consistent with the pathogenic factors and environment of coal pneumoconiosis and the process of occurrence and development of coal worker pneumoconiosis. It is simple and easy to make and evaluate.
【學位授予單位】：吉林大學
【學位級別】：博士
【學位授予年份】：2009
【分類號】：R135.2;R-332

【引證文獻】

相關(guān)期刊論文 前1條

1 劉建永;許海濤;;煤礦作業(yè)工人和塵肺患者誘導(dǎo)痰細胞計數(shù)分析[J];中國保健營養(yǎng);2013年07期

相關(guān)碩士學位論文 前5條

1 侯婧;煤工塵肺大鼠學習記憶能力改變及腦組織中MDA、SOD的水平變化[D];山西醫(yī)科大學;2011年

2 張顯娟;TGF-β1、MMP-2和TIMP-2在煤工塵肺病中的表達及意義[D];山西醫(yī)科大學;2011年

3 赫金鳳;煤礦作業(yè)工人誘導(dǎo)痰中轉(zhuǎn)化生長因子-β1的表達水平[D];鄭州大學;2011年

4 顧志娟;兔煤工塵肺模型HRCT表現(xiàn)與病理學對照研究[D];山西醫(yī)科大學;2012年

5 常紅生;兔煤塵肺各期高分辨率CT表現(xiàn)與肺巨噬細胞含量的相關(guān)研究[D];山西醫(yī)科大學;2013年

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本文編號：1732185