本文選題：煙曲霉菌　切入點：生物膜　出處：《中華醫(yī)院感染學(xué)雜志》2015年06期

【摘要】：目的探討綠原酸(CRA)對早期(24h)、成熟期(48h)煙曲霉菌生物膜(BF)的破壞及其聯(lián)合兩性霉素B(AMB)的殺菌作用,為臨床煙曲霉菌BF相關(guān)性感染的治療提供參考依據(jù)。方法構(gòu)建體外煙曲霉菌24、48h BF模型,分空白組、CRA組、AMB組、CRA+AMB組;兩倍稀釋法測定藥物的最低抑菌濃度(MIC)和最小殺菌濃度(MFC);結(jié)晶紫法定量BF;掃描電鏡(SEM)觀察BF的形態(tài)。結(jié)果受試菌株經(jīng)培養(yǎng)24、48h后可形成穩(wěn)定BF,各組藥物作用48h后BF定量顯示,早期和成熟期BF空白組和單獨AMB組相比差異均無統(tǒng)計學(xué)意義,而早期的CRA組較空白組和AMB組的BF減少(P0.05),與CRA組相比,CRA+AMB組BF則進一步減少(P0.05);成熟期的CRA+AMB組與空白組、單獨AMB組或CRA組比較,BF也明顯減少(P0.05);SEM觀察早期和成熟期BF,CRA組均較空白組和AMB組胞外基質(zhì)明顯減少,CRA+AMB組胞外基質(zhì)和菌絲均顯著減少。結(jié)論綠原酸可以破壞早期和成熟期生物膜,與AMB聯(lián)合可以增強AMB對生物膜的滲透性,與AMB具有協(xié)同殺菌效果。
[Abstract]:Objective to study the bactericidal effects of Lv Yuan on the biological membrane of Aspergillus fumigatus (BFF) in early stage (24 h) and mature stage (48 h) and its combination with amphotericin (Amphotericin). Methods A 48 h BF model of Aspergillus fumigatus in vitro was established and divided into two groups: the control group was divided into two groups: the control group and the control group were divided into two groups: the control group, the control group, the control group, the control group, the control group, the control group and the control group. Two times dilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MFCU). The morphology of BF was observed by crystal violet method and scanning electron microscope (SEM). Results the tested strains could form stable BFs after 24 h culture, and the BF of each group could be quantitatively displayed after 48 h treatment. There was no significant difference between the BF blank group and the AMB group in the early and mature stage, but the BF in the early CRA group was lower than that in the blank group and the AMB group, the BF in the CRA AMB group was further reduced than that in the CRA group, and the CRA AMB group and the blank group in the mature stage. Compared with AMB group and CRA group, AMB group and CRA group also decreased the number of extracellular matrix and hyphae in early and mature stage. Conclusion Lv Yuan can destroy biofilm in early and mature stage, and the extracellular matrix in AMB group is significantly lower than that in control group and AMB group. Combined with AMB can enhance the permeability of AMB to biofilm and synergistic bactericidal effect with AMB.
【作者單位】： 廣西醫(yī)科大學(xué)第一附屬醫(yī)院呼吸疾病研究所;
【基金】：國家自然科學(xué)基金資助項目(81260002)
【分類號】：R379.6

【參考文獻】

相關(guān)期刊論文 前3條

1 溫紅俠;陳一強;朱蓮娜;宋志軍;楊朝生;王恒壯;張東偉;;綠原酸對銅綠假單胞菌生物膜干預(yù)作用的體外研究[J];中華醫(yī)院感染學(xué)雜志;2009年12期

2 李麗娟;陳偉;許輝;萬U，

本文編號：1677286