本文選題：銅綠假單胞菌　切入點：吩嗪　出處：《西北大學》2008年碩士論文　論文類型：學位論文

【摘要】： 銅綠假單胞菌(Pseudomonas aeruginosa)是一個分布廣泛的機會致病菌[1],在燒傷病人、器官移植病人、免疫力低下病人,如接受化學治療的癌癥病人和艾滋病病人中,它幾乎可以感染人體的任何組織并常常危及患者生命。它對許多抗生素具備很高的內在抗藥性,并可產生許多致病因子,如堿性蛋白酶(alkalineprotease)和吩嗪(phenazine)等,是難以治療的病原菌。 綠膿菌素作為吩嗪的一種衍生物,不僅是一種致病因子,更重要的是作為銅綠假單胞菌中的一種信號分子。因此,研究吩嗪合成的調節(jié)途徑對于了解銅綠假單胞菌的致病機制和微生物群體中不同細菌間的相互作用具有重要意義。 本文運用轉座子突變方法,對含有報道質粒miniCTX-phzAl的銅綠假單胞菌進行轉座子隨機突變,篩選影響吩嗪合成的調節(jié)突變體。篩選到了6個影響吩嗪合成的突變體,并對這6個突變體進行了鑒定,確定突變體中轉座子插入位點,從而確定轉座子所破壞的基因。并根據生物信息分析結果,推測其功能。研究結果為了解銅綠假單胞菌致病因子的調節(jié)機理提供了新的數據和線索。 同時,對一個菌落和顏色發(fā)生明顯變化的突變體進行的深入研究表明,該轉座突變的基因是銅綠假單胞菌的一個新轉錄調節(jié)基因。并通過基因敲除的方法驗證了該基因的功能。 采用基因敲除的方法,構建了負責ABC轉運系統(tǒng)的兩個基因PA0602和PA4594基因的突變體。研究這兩個基因是否參與低抑制濃度四環(huán)素對吩嗪合成基因phzAl的調節(jié)。探索了這兩個基因與群體感應體調節(jié)系統(tǒng)和PQS系統(tǒng)之間一定的關系。
[Abstract]:Pseudomonas aeruginosa (Pseudomonas aeruginosa) is a widely distributed opportunistic pathogen in burn patients, organ transplant patients, immunocompromised patients, such as cancer patients receiving chemotherapy and AIDS patients. It can infect almost any human tissue and often endanger the life of patients. It has high intrinsic resistance to many antibiotics and can produce many pathogenic factors, such as alkaline protease, phenazine and phenazine, which are difficult to treat. As a derivative of phenazine, Pseudomonas aeruginosa is not only a pathogenic factor, but also a signaling molecule in Pseudomonas aeruginosa. It is important to study the regulatory pathway of phenazine synthesis for understanding the pathogenic mechanism of Pseudomonas aeruginosa and the interaction between different bacteria in microbial population. In this paper, transposon random mutation of Pseudomonas aeruginosa containing reporter plasmid miniCTX-phzAl was carried out by transposon mutation method to screen regulatory mutants affecting phenazine synthesis. Six mutants affecting phenazine synthesis were screened. The six mutants were identified and the transposon insertion sites were determined to determine the genes destroyed by transposons. The results provide new data and clues for understanding the regulatory mechanism of the pathogenic factors of Pseudomonas aeruginosa. At the same time, an in-depth study of a mutant with significant changes in colony and color showed that, The translocation mutant gene is a new transcriptional regulator of Pseudomonas aeruginosa and its function is verified by gene knockout. Using gene knockout, The mutants of two genes, PA0602 and PA4594, responsible for the ABC transport system, were constructed. Whether the two genes were involved in the regulation of phenazine synthesis gene phzAl by low inhibitory concentration tetracycline was studied. There is a certain relationship between the regulating system and the PQS system.
【學位授予單位】：西北大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R378

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