本文選題：細(xì)胞滾動黏附　切入點(diǎn)：小波分析　出處：《華南理工大學(xué)》2010年碩士論文　論文類型：學(xué)位論文

【摘要】： 細(xì)胞滾動黏附是血栓形成和炎癥反應(yīng)過程中的重要階段。揭示細(xì)胞滾動黏附的物理過程,對于深入了解白細(xì)胞如何在血流剪切作用下到達(dá)炎癥部位,血小板如何發(fā)生黏附和聚集等相關(guān)的生理病理過程,以及對抗炎、抗腫瘤、溶解血栓等藥物的設(shè)計(jì),均具有十分重要的意義。本論文以E-選擇素/PSGL-1調(diào)控的HL-60細(xì)胞黏附為研究對象,以流動腔實(shí)驗(yàn)技術(shù)和小波分析為手段,研究了細(xì)胞滾動黏附過程中的熱響應(yīng)動力學(xué)問題。其主要內(nèi)容包括以下三個方面: 一、通過小波分析研究滾動黏附的物理過程。流動腔實(shí)驗(yàn)技術(shù)是研究流體環(huán)境下細(xì)胞-分子作用的重要技術(shù),可獲得納米級別的分子信息,但由于其在實(shí)驗(yàn)過程中,會受到細(xì)胞的不規(guī)則性、流場的局部擾動、實(shí)驗(yàn)系統(tǒng)誤差等因素的影響,實(shí)驗(yàn)結(jié)果不可避免地包含了一定的噪聲波動。本文通過對細(xì)胞運(yùn)動過程中的噪聲的統(tǒng)計(jì)分析,確定了對應(yīng)的小波分析方法,對細(xì)胞的運(yùn)動過程進(jìn)行了降噪處理,準(zhǔn)確識別出了原始信號中細(xì)胞黏附和解離的時刻,并根據(jù)降噪后的數(shù)據(jù)結(jié)果判斷細(xì)胞運(yùn)動過程中的瞬時黏附-解離事件。 二、建立細(xì)胞自由狀態(tài)和拴縛狀態(tài)的熱響應(yīng)動力學(xué)模型。通過朗之萬方程和能量均分定理,對細(xì)胞自由狀態(tài)的能量特性進(jìn)行描述,并結(jié)合拴縛細(xì)胞的振子反應(yīng)系統(tǒng)建立模型,推導(dǎo)出拴縛細(xì)胞在受流體和分子鍵作用下的能量分布。為了解決由于受到實(shí)驗(yàn)條件限制而出現(xiàn)的采樣時間間隔不等的問題,本文分析了熱響應(yīng)信號檢測的時間尺度效應(yīng)問題,以方便對實(shí)驗(yàn)數(shù)據(jù)進(jìn)行統(tǒng)一化分析。 三、對細(xì)胞運(yùn)動過程中的噪聲來源和能量特性進(jìn)行分析。通過對細(xì)胞運(yùn)動狀態(tài)的各物理量進(jìn)行小波分析,分解其不同運(yùn)動階段的細(xì)節(jié)分量,對不同的變化趨勢給出相應(yīng)的物理解釋,建立分子鍵空間約束和振子系統(tǒng)雙效應(yīng)模型;通過對速度分量進(jìn)行三層分解,對各種可能的噪聲因素進(jìn)行分離,找到了不同頻率噪聲可能的來源;分別對X和Y單方向速度進(jìn)行三層分解,結(jié)合總速度分層得到的噪聲來源,驗(yàn)證了拴縛細(xì)胞的熱響應(yīng)模型中提出的能量分布。 本文確立了小波分析方法對流動腔實(shí)驗(yàn)數(shù)據(jù)降噪的有效性,建立并驗(yàn)證了細(xì)胞運(yùn)動過程中自由和拴縛階段的熱響應(yīng)動力學(xué)模型,對細(xì)胞滾動黏附問題的進(jìn)一步研究具有重要的作用。
[Abstract]:Cell rolling adhesion is an important stage in the process of thrombosis and inflammatory reaction. To reveal the physical process of cell rolling adhesion, it is important to understand how white blood cells reach the inflammatory site under the action of blood flow shear. How platelets develop the physiological and pathological processes associated with adhesion and aggregation, as well as the design of drugs such as anti-inflammation, anti-tumor, thrombolysis, etc. In this thesis, the adhesion of HL-60 cells regulated by Eselectin / PSGL-1 was studied, and the flow chamber technique and wavelet analysis were used to study the adhesion of E- selectin / PSGL-1-regulated HL-60 cells. The thermal response kinetics in the process of cell rolling adhesion is studied. The main contents include the following three aspects:. First, the physical process of rolling adhesion is studied by wavelet analysis. The flow chamber experimental technique is an important technique to study cell-molecular interaction in fluid environment. It will be influenced by the irregularity of cell, the local disturbance of flow field, the error of experimental system and so on. The experimental results inevitably contain certain noise fluctuation. The corresponding wavelet analysis method is determined, and the noise reduction of the cell motion process is carried out, and the time of cell adhesion and dissociation in the original signal is accurately identified. The transient adhesion-dissociation events in the course of cell motion were judged according to the data after noise reduction. Secondly, the dynamic models of thermal response of free state and binding state of cells are established. The energy characteristics of free state of cells are described by Langevin equation and energy equalization theorem, and the model of the vibrator reaction system of the tied cells is established. In order to solve the problem of unequal sampling time interval due to the limitation of experimental conditions, the time-scale effect of thermal response signal detection is analyzed in this paper. In order to facilitate the unified analysis of experimental data. Third, the source of noise and energy characteristics during cell motion are analyzed. By wavelet analysis of the physical quantities in the state of cell motion, the detailed components in different motion stages are decomposed. The corresponding physical explanation of different trends is given, and the double effect model of molecular bond space constraint and vibration subsystem is established, and various possible noise factors are separated by three-layer decomposition of velocity component. The possible sources of noise at different frequencies are found and the energy distribution proposed in the thermal response model of the bundled cells is verified by decomposing X and Y unidirectional velocities into three layers and combining the noise sources obtained from the total velocity stratification. In this paper, the validity of wavelet analysis to the noise reduction of flow chamber experimental data is established, and the dynamic model of thermal response in the free and bundled stages of cell motion is established and verified. It is important to further study the problem of cell rolling adhesion.
【學(xué)位授予單位】：華南理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R364

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