本文選題：滇藍(lán)尾蠑螈　切入點：肢體　出處：《昆明醫(yī)學(xué)院》2009年碩士論文　論文類型：學(xué)位論文

【摘要】： [目的]:建立再生生物學(xué)研究的理想模型,觀察滇藍(lán)尾蠑螈前肢肢體的割處再生能力,通過組織形態(tài)學(xué)觀察,了解局部細(xì)胞的去分化現(xiàn)象及其細(xì)胞來源。 [方法]:取野生成體雌性及雄性滇藍(lán)尾蠑螈共15條,麻醉下自肘部稍下切除右側(cè)前肢,術(shù)后3、7、12、15、20、30、45天切取創(chuàng)部新生組織,通過大體形態(tài)、組織切片觀察肢體的再生以及再生過程中局部組織細(xì)胞的形態(tài)學(xué)變化和再生組織的細(xì)胞來源。 [結(jié)果]:所有實驗動物均健康成活,被切除的右前肢均能再生出缺損的肢體,其過程如下:術(shù)后3天,形成創(chuàng)面表皮封閉,也稱為頂帽或頂外胚層帽(apicalectodermal cap),頂帽下方存在一群圓形的單個核細(xì)胞沿殘肢近遠(yuǎn)中軸生長,同時可見狹長的骨骼肌細(xì)胞及成纖維細(xì)胞向遠(yuǎn)端移行,有些細(xì)胞胞核可見特異肌源性蛋白Myogenin陽性表達(dá);術(shù)后7～15天天,頂帽下方形成再生芽基(blastema),細(xì)胞呈現(xiàn)幼稚化,核仁明顯,細(xì)胞體小,核質(zhì)比變大,Myogenin陽性表達(dá)增多;大約20～30天天,芽基細(xì)胞轉(zhuǎn)分化形成成骨細(xì)胞,骨骼肌細(xì)胞等,部分成骨細(xì)胞胞核有Myogenin陽性表達(dá),最終約45天細(xì)胞再分化構(gòu)成一個新的肢體,只是新生肢體較原切除前肢在色澤大小上略有差異。 [結(jié)論]:(1)滇藍(lán)尾蠑螈前肢具有完全的“割處再生”能力,可作為再生生物學(xué)研究的理想動物模型;(2)滇藍(lán)尾蠑螈前肢的割處再生是鄰近組織如骨骼肌細(xì)胞遷移、去分化、增殖并再分化的結(jié)果;(3)滇藍(lán)尾蠑螈前肢再生芽基中,骨骼肌源性特異性蛋白Myogenin的表達(dá)為強陽性,提示骨骼肌細(xì)胞是再生芽基的細(xì)胞來源之一。
[Abstract]:[objective] to establish an ideal model of regenerative biology, to observe the regeneration ability of the forelimb of salamander, and to understand the dedifferentiation of local cells and the origin of the cells by histomorphological observation. [methods] Fifteen wild adult female and male salamanders were removed from the right forelimb slightly below the elbow under anesthesia. After 3 days of operation, the neovascularization tissues of the trauma region were cut out at 30 and 45 days after operation, and the gross morphology was adopted. Tissue sections were used to observe the regeneration of limbs, the morphological changes of local histocytes and the origin of regenerated tissues. [results]: all the experimental animals survived healthily, and all the right forelimbs resected could regenerate the defective limbs. The process was as follows: 3 days after operation, the wound epidermis was closed. Also known as the top cap or apical ectodermal cap, a group of circular mononuclear cells grow along the distal axis of the remnant limb beneath the cap, and long narrow skeletal muscle cells and fibroblasts can be seen migrating to the distal end. Positive expression of specific myogenic protein Myogenin was found in some cell nuclei. After 7 days, 15 days after operation, regenerated blastocyst was formed under the top cap. The cells were immature, nucleoli were obvious, the cell body was small, and the positive expression of Myogenin was increased with the increase of the ratio of nucleus to cytoplasm; about 20 to 30 days after operation, the positive expression of Myogenin was found in the nucleus of some cells. Budding cells were transformed into osteoblasts, skeletal muscle cells and other osteoblasts. Some osteoblasts showed Myogenin positive expression in their nuclei. Finally, the cells were redifferentiated for about 45 days to form a new limb. However, the color of the new limb was slightly different from that of the original forelimb. [conclusion] the forelimb of the salamander has the ability to regenerate completely and can be used as an ideal animal model for the study of regenerative biology. The regeneration of the forelimb of the salamander is the migration and dedifferentiation of adjacent tissues such as skeletal muscle cells. The expression of skeletal myogenic specific protein (Myogenin) was strongly positive in the regenerated bud of the forelimb of Salamander, suggesting that skeletal muscle cells are one of the cell sources of regenerated bud.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R-332

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 王芳;渦蟲再生相關(guān)差異蛋白質(zhì)組的研究[D];鄭州大學(xué);2010年

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本文編號：1608814