本文選題：神經(jīng)元　切入點：脈沖磁場　出處：《天津大學(xué)》2008年碩士論文　論文類型：學(xué)位論文

【摘要】： 隨著現(xiàn)代工業(yè)的發(fā)展,磁場對人體的影響日趨廣泛且不明確,同時也有越來越多的磁療儀器產(chǎn)生,并且已應(yīng)用于臨床治療,但其治療機(jī)理尚不清楚,有待于進(jìn)一步探明。因此研究磁場對細(xì)胞的作用具有現(xiàn)實的重要意義。 本文首次從細(xì)胞層次上觀察了在低強度低頻脈沖磁場環(huán)境下,小鼠皮層神經(jīng)元電壓門控鈉鉀離子通道電生理特性的變化。采用頻率為15Hz、強度為1.4mT的低強度低頻脈沖磁場對小鼠的腦皮層神經(jīng)元進(jìn)行刺激,而后應(yīng)用全細(xì)胞膜片鉗技術(shù)測量其鈉鉀離子通道特性。結(jié)果顯示:低強度低頻脈沖磁場可使神經(jīng)元電壓門控離子通道特性改變,可顯著影響皮層神經(jīng)元離子通道激活電位和通道電流,對INa、IA、IK的作用具有電壓依賴性。脈沖磁場作用于小鼠皮層神經(jīng)元對鈉通道峰值電流INa起到明顯的抑制作用,并且其改變了鈉通道的激活特性與失活特性,從而影響動作電位的去極化與超極化過程,進(jìn)而會引起神經(jīng)元細(xì)胞生理功能發(fā)生變化。同時脈沖磁場對瞬時外向鉀電流IA和延遲整流外向鉀電流IK有顯著的抑制作用,脈沖磁場作用可顯著地影響外向鉀通道的激活與失活過程,可以改變其瞬時外向鉀通道特性,調(diào)節(jié)神經(jīng)元的生理功能,可能有利于受損神經(jīng)元的恢復(fù)。 應(yīng)用膜片鉗技術(shù)在細(xì)胞和分子水平探討低強度低頻脈沖磁場的生物刺激效應(yīng)是一個全新的方法,本文從離子通道角度上研究脈沖磁場對腦皮層神經(jīng)元的影響,為全面深入地探索電磁場的生物效應(yīng)提供了借鑒。
[Abstract]:With the development of modern industry, the influence of magnetic field on human body is becoming more and more extensive and unclear. At the same time, more and more magnetic therapy instruments have been produced, and have been used in clinical treatment, but its therapeutic mechanism is not clear. Therefore, it is important to study the effect of magnetic field on cells. In this paper, we have for the first time observed the low intensity low-frequency pulsed magnetic field at the cellular level. Changes of electrophysiological characteristics of voltage-gated sodium and potassium channels in mouse cortical neurons stimulation of mouse cortical neurons by low-intensity low-frequency pulsed magnetic field with frequency of 15 Hz and intensity of 1.4 Mt. The characteristics of sodium and potassium channels were measured by whole-cell patch clamp technique. The results show that low-intensity low-frequency pulsed magnetic field can change the characteristics of voltage-gated ion channels of neurons. It can significantly affect the ion channel activation potential and channel current of cortical neurons, and has a voltage-dependent effect on INAIAIK. The effect of pulsed magnetic field on the peak sodium channel current (INa) in cortical neurons of mice was significantly inhibited. Moreover, it changes the activation and inactivation characteristics of sodium channel, thus affecting the depolarization and hyperpolarization of action potential. At the same time, pulse magnetic field can inhibit transient outward potassium current (IA) and delayed rectifier outward potassium current (IK). The action of pulsed magnetic field can significantly affect the activation and inactivation of outward potassium channel, change its transient outward potassium channel characteristics, regulate the physiological function of neurons, and may be beneficial to the recovery of injured neurons. It is a new method to investigate the biological stimulation effect of low-intensity low-frequency pulsed magnetic field at cell and molecular level by using patch clamp technique. In this paper, the effect of pulsed magnetic field on cortical neurons was studied from the angle of ion channel. It provides a reference for exploring the biological effects of electromagnetic field.
【學(xué)位授予單位】：天津大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R312

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 陳新光;檢測神經(jīng)纖維膜電位動力學(xué)的光學(xué)二次諧波編碼及其數(shù)值模擬[D];福建師范大學(xué);2012年

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本文編號：1604059