本文選題：身心應(yīng)激　切入點(diǎn)：卵巢早衰　出處：《華中科技大學(xué)》2010年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過給予雌性SD大鼠長(zhǎng)期的身心應(yīng)激,建立大鼠POF模型,觀察大鼠動(dòng)情周期、行為癥狀改變、神經(jīng)內(nèi)分泌的改變以及卵巢局部因子的變化,研究身心應(yīng)激引起POF的機(jī)制。 方法:將SD雌性大鼠隨機(jī)分為正常組和模型組。正常組不做任何處理,常規(guī)喂養(yǎng)。模型組采用改良國(guó)際應(yīng)激方法,每天隨機(jī)抽取一種方法給予應(yīng)激,應(yīng)激每一籠模型組大鼠時(shí),將其余模型組大鼠放于旁邊,共同處于應(yīng)激氣氛中,以達(dá)到心理應(yīng)激的目的。模型組連續(xù)應(yīng)激28天,其間觀察大鼠行為及最后十天觀察動(dòng)情周期變化,于第29天時(shí)同時(shí)處死正常組和模型組,收集大鼠卵巢、血清以及腦組織,采用HE染色法觀測(cè)卵巢病理學(xué)改變,免疫組織化學(xué)法測(cè)定卵巢bax和VEGF蛋白的含量,放免法測(cè)定血清FSH和E2的含量,ELISA法檢測(cè)NE、皮質(zhì)酮和INHB的含量以及Realtime-PCR法檢測(cè)卵巢GDF-9B mRNA的表達(dá)。 結(jié)果:行為癥狀顯示,與正常組相比,模型組大鼠皮毛枯燥易掉,行動(dòng)減少、呆滯,抓咬減少,洗臉次數(shù)增多,體重減輕。與正常組相比,HE染色卵巢病理學(xué)改變顯示模型組大鼠各級(jí)正常卵泡明顯減少(P0.05),閉鎖卵泡顯著增多(P0.01),黃體數(shù)目顯著減少(P0.01),且體積大小不均;與正常組相比,模型組神經(jīng)內(nèi)分泌改變顯示血清FSH含量升高(P0.01),E2含量降低(P0.05);模型組下丘腦NE值有下降趨勢(shì)(P0.05),垂體NE值降低(P0.05),血清NE值升高(P0.01);血清皮質(zhì)酮顯著升高(P0.01);血清INHB顯著降低(P0.01);與正常組相比,模型組卵巢bax蛋白表達(dá)高于正常組(P0.01);VEGF蛋白低于正常組(P0.01);模型組卵巢GDF-9B mRNA表達(dá)有下降趨勢(shì),但差異無統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論: 1 .身心應(yīng)激能導(dǎo)致部分雌鼠卵巢早衰,是卵巢早衰的重要病因之一。 2.身心應(yīng)激可引起精神-神經(jīng)-內(nèi)分泌紊亂以致神經(jīng)遞質(zhì)分泌失調(diào),導(dǎo)致下丘腦-垂體-腎上腺軸和下丘腦-垂體-卵巢軸破壞失衡,導(dǎo)致卵巢早衰。 3.身心應(yīng)激可下調(diào)雌鼠卵巢血管內(nèi)皮生長(zhǎng)因子,減少卵巢血管發(fā)生,使卵巢血供減少,改變卵巢細(xì)胞內(nèi)環(huán)境,增加顆粒細(xì)胞凋亡因子bax表達(dá),影響卵母細(xì)胞GDF-9B mRNA表達(dá),使卵泡閉鎖加速,最終共同作用導(dǎo)致卵巢早衰。
[Abstract]:Objective: to study the mechanism of POF induced by physical and mental stress in female SD rats by observing the estrous cycle, behavioral changes, neuroendocrine changes and changes of local factors of ovary. Methods: SD female rats were randomly divided into normal group and model group. When each cage model group rats were stressed, the rest of the model group rats were placed on the side, together in the stress atmosphere, in order to achieve the purpose of psychological stress. The model group was under continuous stress for 28 days, and the rats in the model group were subjected to stress for 28 days. The behavior of rats and the changes of estrus cycle in the last ten days were observed. On the 29th day, the normal group and the model group were killed at the same time, the ovary, serum and brain tissues were collected, and the pathological changes of the ovary were observed by HE staining. The contents of bax and VEGF protein in ovary were detected by immunohistochemistry, the contents of FSH and E2 in serum were detected by radioimmunoassay, the contents of INHB and corticosterone were detected by Elisa, and the expression of GDF-9B mRNA in ovary was detected by Realtime-PCR method. Results: the behavioral symptoms showed that compared with the normal group, the fur of the model group was dull and easy to lose, the movement decreased, the grip decreased, the number of facial washing increased. Compared with the normal group, the pathological changes of the ovary stained by HE staining showed that the normal follicles in the model group decreased significantly (P 0.05), the atresia follicles increased significantly (P 0.01), the number of corpus luteum decreased significantly (P 0.01), and the volume was uneven, compared with the normal group. The neuroendocrine changes in the model group showed that the serum FSH content was increased P0.01E _ 2 content was decreased, the hypothalamus NE value was decreased, the pituitary NE value was decreased, the serum NE value was increased, the serum NE value was increased, the serum corticosterone level was significantly increased, and the serum INHB was significantly decreased. Compared with normal group, The expression of bax protein in the model group was higher than that in the normal group, and the expression of GDF-9B mRNA in the model group was lower than that in the normal group, but there was no significant difference in the expression of GDF-9B mRNA between the model group and the normal group (P 0.05). Physical and mental stress can lead to premature ovarian failure in some female rats, which is one of the important causes of premature ovarian failure. 2. 2. Mental and physical stress can lead to the disorder of neurotransmitter secretion, the destruction and imbalance of hypothalamus-pituitary-adrenal axis and hypothalamus-pituitary-ovarian axis, and premature ovarian failure. 3. Physical and mental stress could down-regulate vascular endothelial growth factor (VEGF), decrease ovarian angiogenesis, decrease ovarian blood supply, change the intracellular environment of ovary, increase the expression of apoptotic factor bax in granulosa cells, and affect the expression of GDF-9B mRNA in oocytes. Accelerate follicular atresia and eventually lead to premature ovarian failure.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R-332

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本文編號(hào)：1595885