本文選題：壓力性尿失禁　切入點：動物模型　出處：《山西醫(yī)科大學》2009年碩士論文　論文類型：學位論文

【摘要】： 1.研究目的 1.1建立壓力性尿失禁的動物模型; 1.2研究轉化生長因子β-3(Tgfβ-3),層粘連蛋白(Lamin),松弛素(Relaxin)在SUI大鼠和正常大鼠陰道前壁和肛提肌組織中的表達情況; 1.3分析三種因子含量在壓力性尿失禁動物模型組和正常對照組中表達含量的變化,初步探討該模型形成的機制以及三種因子與壓力性尿失禁發(fā)生發(fā)展的關系,為臨床發(fā)病機制、診斷和治療提供一定的實驗室依據。 2.實驗方法 2.1將50只雌性SD大鼠分為五組:Ⅰ組為正常對照組(10只);Ⅱ組為正常分娩組(10只);Ⅲ組為正常分娩+陰道擴張組(10只);Ⅳ組為正常分娩+卵巢切除組(10只);Ⅴ組為正常分娩+陰道擴張+卵巢切除組(10只); 2.2進行尿動力學檢測,以證實壓力性尿失禁; 2.3實驗結束后,取實驗證實的SUI大鼠與正常大鼠的陰道前壁和肛提肌,RT-PCR分別檢測大鼠陰道前壁和肛提肌中三種因子Tgfβ-3、Lamin和Relaxin mRNA含量的表達,從而比較壓力性尿失禁動物模型組和正常組中三種因子含量表達的變化。 3.實驗結果 3.1 4個月中共4只大鼠死亡,Ⅰ組腹漏尿點壓(ALPP)為(74.24±5.01) cmH2O,Ⅱ組ALPP為(70.81±3.06)cm H2O,Ⅲ組ALPP為(52.62±3.63)cm H2O,Ⅳ組ALPP為(53.26±3.45)cmH2O,Ⅴ組ALPP為(35.20±3.61)cmH2O。 3.2Ⅱ組的ALPP低于Ⅰ組,但二者比較無統(tǒng)計學意義(P0.05);Ⅲ組、Ⅳ組和Ⅴ組的ALPP均低于Ⅰ組,兩兩比較有統(tǒng)計學意義(P0.05);Ⅲ組、Ⅳ組兩者ALPP比較無統(tǒng)計學意義(P0.05);Ⅱ組、Ⅲ組、Ⅳ組與Ⅴ組ALPP兩兩相比差異均有統(tǒng)計學意義(P0.05)。 3.3Ⅴ組中的SUI模型鼠陰道前壁中轉化生長因子β-3(Tgfβ-3),層粘連蛋白(Lamin)二種因子的表達含量較Ⅰ組有明顯的降低,差異有統(tǒng)計學意義(P0.05);松弛素(Relaxin)因子的表達含量較Ⅰ組有明顯的升高,差異有統(tǒng)計學意義(P0.05)。 3.4Ⅴ組中SUI模型鼠肛提肌中轉化生長因子β-3(Tgfβ-3),層粘連蛋白(Lamin)二種因子的表達含量較Ⅰ組有明顯的降低,差異有統(tǒng)計學意義(P0.01);松弛素(Relaxin)因子的表達含量較Ⅰ組有明顯的升高,差異有統(tǒng)計學意義(P0.01)。 4.結論 4.1模擬妊娠、難產、雌激素減退可以成功建立壓力性尿失禁動物模型,同時模擬幾種病因成功率更高。 4.2壓力性尿失禁動物模型中陰道前壁組織中轉化生長因子β-3(Tgfβ-3),層粘連蛋白(Lamin)二種因子的表達含量較正常無壓力性尿失禁組有明顯的降低,松弛素(Relaxin)表達含量有明顯的升高。 4.3壓力性尿失禁動物模型中肛提肌中轉化生長因子β-(3Tgfβ-3),層粘連蛋白(Lamin)二種因子的表達含量較正常無壓力性尿失禁組有明顯的降低,松弛素(Relaxin)表達含量有明顯的升高。提示這Tgfβ-3 ,Lamin,Relaxin與SUI發(fā)展有相關性。
[Abstract]:1. purpose of research
1.1 animal model of stress urinary incontinence was established.
1.2, we studied the expression of transforming growth factor beta -3 (Tgf beta -3), laminin (Lamin) and relaxin (Relaxin) in the anterior vaginal wall and the levator ani muscle of SUI rats and normal rats.
1.3 factor analysis of three kinds of content changes in expression of urinary stress incontinence animal model group and normal control group, to investigate the relationship between the formation mechanism of this model and three kinds of factors and stress urinary incontinence development, clinical pathogenesis, and provide laboratory evidence for a diagnosis and treatment.
2. experimental method
2.1 50 female SD rats were randomly divided into five groups: normal control group (10 rats); group II, normal delivery group (10 rats); group III normal delivery + colpectasis group (10 rats); group IV normal delivery + ovariectomy group (10 rats); V group of normal delivery + colpectasis + ovariectomized group (10 rats);
2.2 the urodynamic test was carried out to confirm the pressure urinary incontinence.
2.3 after the end of the experiment, the experiment proved SUI rats and normal rats anterior vaginal wall and levator ani muscle, RT-PCR was used to detect the anterior wall of vagina and anus levator muscle of rats in three kinds of factor Tgf beta -3, Lamin expression and Relaxin content of mRNA, so as to compare group urinary stress incontinence animal model and three a factor in the changes of the expression of the normal group.
3. experimental results
In 3.14 months, 4 rats died. The abdominal leak point pressure (ALPP) in group I was (74.24 + 5.01) cmH2O, group ALPP was (70.81 + 3.06) cm H2O, group III ALPP (52.62 + 3.63) cm H2O, group IV ALPP (53.26 3.45 3.45) cmH2O, group V ALPP (35.20 + 35.20) cmH2O.
The 3.2 group II ALPP lower than that of group I, but there was no significant difference between the two (P0.05); group III, IV and V group group ALPP were lower than that in group I, 22 were statistically significant (P0.05); group III, IV group two ALPP was not statistically significant (P0.05); group II, group III in group IV, V and 22 ALPP group had a significant difference (P0.05).
3.3鈪ょ粍涓殑SUI妯″瀷榧犻槾閬撳墠澹佷腑杞寲鐢熼暱鍥犲瓙尾-3(Tgf尾-3),灞傜矘榪炶泲鐧，

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