本文選題：白細(xì)胞介素　切入點：多態(tài)性　出處：《天津醫(yī)藥》2015年08期 　論文類型：期刊論文

【摘要】：目的分析白細(xì)胞介素(IL)-12A基因單核苷酸多態(tài)性(SNP)位點rs568408和IL-12B基因SNP位點rs3212227的各基因型和等位基因在廣西壯族人群中的分布頻率,及不同地區(qū)的分布差異。方法采用單堿基延伸的PCR技術(shù)對165例廣西壯族人群IL-12A rs568408和IL-12B rs3212227進行多態(tài)性檢測,對比國際人類基因組計劃(Hap Map)公布的中國北京、日本、非洲和意大利人群的SNP分型數(shù)據(jù),分析5個人群rs568408、rs3212227位點的基因型和等位基因頻率差異。結(jié)果在廣西壯族人群中存在rs568408和rs3212227多態(tài)性,2位點基因型及等位基因頻率在男女性別間差異無統(tǒng)計學(xué)意義(P0.05),IL-12A rs568408與北京、日本和意大利人群差異無統(tǒng)計學(xué)意義(P0.05),但與非洲人群存在差異(P0.01);IL-12B rs3212227與北京和日本人群差異無統(tǒng)計學(xué)意義,但與非洲和意大利人群存在差異(P0.01)。結(jié)論 IL-12A rs568408和IL-12B rs3212227存在多態(tài)性,2位點基因型和等位基因在廣西壯族人群中的分布頻率與其他種族和地區(qū)人群相比存在差異,這種差異可能是導(dǎo)致某些疾病在不同人群發(fā)病率和臨床表現(xiàn)存在差異的原因之一。
[Abstract]:Objective to analyze the distribution frequency of the genotypes and alleles of rs568408 and IL-12B SNP rs3212227 in Guangxi Zhuang population. Methods the polymorphisms of IL-12A rs568408 and IL-12B rs3212227 in 165 Zhuang people in Guangxi were detected by single base extension PCR technique, and compared with those of Beijing, Japan, China published by the International Human Genome Project. SNP typing data from African and Italian populations, The genotype and allelic frequency of rs568408 / rs3212227 locus were analyzed. Results there was no significant difference in genotype and allele frequency of rs568408 and rs3212227 polymorphism locus 2 between male and female in Guangxi Zhuang population, and there was no significant difference between male and female in IL-12A rs568408 between male and female. There was no significant difference between Japanese and Italian population (P 0.05), but there was no significant difference in IL-12B rs3212227 between Japanese and African population compared with that in Beijing and Japan. Conclusion IL-12A rs568408 and IL-12B rs3212227 are polymorphic loci genotype and allele in Guangxi Zhuang population, compared with other ethnic groups and regions, there are differences in the distribution frequency of genotype and allele between Guangxi Zhuang and other ethnic groups and regions. This difference may be one of the reasons for the differences in the incidence and clinical manifestations of some diseases in different populations.
【作者單位】： 右江民族醫(yī)學(xué)院附屬醫(yī)院脊柱外科;
【基金】：國家自然科學(xué)基金資助項目(81260315) 廣西自然科學(xué)基金項目(2012GXNSFAA053169) 廣西衛(wèi)生廳醫(yī)療衛(wèi)生重點科研課題項目(重2010059) 廣西衛(wèi)生廳自籌經(jīng)費科研課題項目(Z2012712)
【分類號】：R394

【共引文獻】

相關(guān)期刊論文 前6條

1 綦輝;申阿東;;免疫基因多態(tài)性與結(jié)核病易感相關(guān)性的研究進展[J];標(biāo)記免疫分析與臨床;2013年05期

2 胡世玲;陳維賢;;白細(xì)胞介素12基因多態(tài)性與結(jié)核易感性的關(guān)系[J];國際檢驗醫(yī)學(xué)雜志;2014年03期

3 戴W歐，

本文編號：1567052