本文選題：裸鼠　切入點：卵巢癌　出處：《重慶醫(yī)科大學》2010年碩士論文　論文類型：學位論文

【摘要】： 背景與目的卵巢癌仍是病死率最高的婦科惡性腫瘤。理想的動物模型對于卵巢癌生物學行為及治療方法的研究等方面均有重要作用。腫瘤原位模型因具有相對較高的仿真性而成為動物實驗研究的首選。本研究選擇人卵巢癌高轉移細胞株HO-8910PM作為成瘤細胞,擬建立裸鼠卵巢癌原位移植瘤模型,并對相關生物學特性進行研究。 方法首先,將HO-8910PM細胞(約2×106個細胞)注射到雌性裸鼠皮下。待皮下成瘤后再通過顯微外科手術將瘤體小塊轉移到其它裸鼠卵巢內(nèi),追蹤觀察原位腫瘤的生長及體內(nèi)的轉移情況,在荷瘤鼠處于瀕死狀態(tài)時將其處死,取原位及轉移病灶處的腫瘤組織作光鏡及電鏡的病理檢查。 結果共有14只裸鼠接受了原位移植手術,經(jīng)追蹤觀察,有8只原位成瘤,成功率為57.14%。通過尸檢發(fā)現(xiàn)有5只荷瘤鼠出現(xiàn)了遠處器官的轉移(轉移率為83.33%),轉移器官為肺,肝臟、膈肌、胃表面、腸管表面、胰臟、腹膜、對側卵巢、髂窩淋巴結等處,以膈肌和肝臟較為普遍。 結論本研究成功地建立了裸鼠原位移植瘤模型,其生物學特性在某種程度上與人類卵巢癌更為相似,為卵巢腫瘤的研究提供了一種動物模型。
[Abstract]:Background & objective Ovarian cancer is still the most fatal gynecologic malignant tumor. The ideal animal model plays an important role in the study of biological behavior and treatment of ovarian cancer. In this study, the human ovarian cancer cell line HO-8910PM was selected as the tumor-forming cell. To establish an orthotopic transplanted tumor model of ovarian cancer in nude mice and to study its biological characteristics. Methods first, HO-8910PM cells (about 2 脳 106 cells) were injected subcutaneously into female nude mice. After subcutaneous tumorigenesis, the tumor was transferred to the ovary of other nude mice by microsurgery. The tumor growth and metastasis in vivo were observed, and the tumor tissues in situ and metastases were examined by light microscope and electron microscope when the tumor-bearing mice were on the verge of death. Results A total of 14 nude mice underwent orthotopic transplantation, 8 of them underwent orthotopic transplantation, and the success rate was 57.14.The distant organ metastasis was found in 5 tumor-bearing mice by autopsy (the metastasis rate was 83.33), and the metastatic organs were lung and liver. Diaphragm, stomach surface, intestinal canal surface, pancreas, peritoneum, contralateral ovary, iliac fossa lymph nodes are more common in diaphragm and liver. Conclusion the model of orthotopic transplanted tumor in nude mice was successfully established in this study, and its biological characteristics were more similar to those of human ovarian cancer to some extent, which provided an animal model for the study of ovarian tumor.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R737.31;R-332

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