本文選題：七氟醚　切入點(diǎn)：異氟醚　出處：《廣西醫(yī)科大學(xué)》2010年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 目的觀察七氟醚、異氟醚預(yù)處理對(duì)大鼠肝臟缺血再灌注損傷以及能量代謝的影響。 方法選擇SD雄性大鼠60只,體重240～250g,隨機(jī)分為三組,肝臟缺血再灌注組(IR),異氟醚預(yù)處理組(Iso),七氟醚預(yù)處理組(Sev),每組20只。IR組:制作可靠的肝缺血模型,肝臟缺血30min,于肝臟缺血前(T_1)、肝臟缺血后再灌注30min(T_2)、120min(T_3)測(cè)各組大鼠血清谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)乳酸脫氫酶(LDH)、腫瘤壞死因子-α(TNF-α)、白介素-1β(IL-1β)的含量以及T_3時(shí)點(diǎn)肝臟組織中谷胱甘肽還原酶(GR)、丙二醛(MDA)的含量、超氧化物歧化酶(SOD)活性以及肝臟的ATP含量,留取部分肝臟做HE病理染色。Sev組:與相應(yīng)麻醉氣體揮發(fā)罐相連接的密閉容器,監(jiān)測(cè)密閉容器的七氟醚濃度,當(dāng)七氟醚濃度穩(wěn)定于2%,將大鼠置入密閉容器內(nèi)保持自主呼吸30min,然后取出大鼠晾于空氣中10min,余步驟同IR組;Iso組:與相應(yīng)麻醉氣體揮發(fā)罐相連接的密閉容器,監(jiān)測(cè)密閉容器的異氟醚濃度,當(dāng)異氟醚濃度穩(wěn)定于1.5%,將大鼠置入密閉容器內(nèi)保持自主呼吸30min,然后取出大鼠晾于空氣中10min,余步驟同IR組。 結(jié)果IR組、Sev組、Iso組分別與T_1時(shí)點(diǎn)相比較T_2、T_3時(shí)點(diǎn)血清ALT、AST、LDH明顯升高,差異有顯著性(P0.05);Sev組和Iso組分別與IR組相比較在T_1時(shí)點(diǎn)血清ALT、AST、LDH差異無(wú)顯著性(P0.05),Sev組和Iso組分別與IR組相比較在T_2、T_3時(shí)點(diǎn)血清ALT、AST、LDH明顯降低,差異有顯著性(P0.05);IR組、Sev組、Iso組分別與T_1時(shí)點(diǎn)相比較T_2、T_3時(shí)點(diǎn)血清TNF-α、IL-1β明顯升高,差異有顯著性(P0.05);Sev組與IR組相比較在T_2、T_3時(shí)點(diǎn)血清白細(xì)胞介素-1β明顯降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05),Iso組與IR組相比較在T_2、T_3時(shí)點(diǎn)血清IL-1β差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);Sev組和Iso組分別與IR組相比較在T_2、T_3時(shí)點(diǎn)血清TNF-α差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);Sev組、Iso組分別與IR組相比較在T_3時(shí)點(diǎn)肝組織MDA明顯降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05),Sev組、Iso組分別與IR組相比較在T_3時(shí)點(diǎn)肝組織SOD、GR明顯升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05);Sev組、Iso組分別與IR組相比較在T_3時(shí)點(diǎn)肝臟組織ATP含量明顯升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05);肝臟病理與正常肝組織相比較,發(fā)生缺血相應(yīng)改變,Sev組、Iso組、IR組間差異不明顯。 結(jié)論七氟醚和異氟醚預(yù)處理能減輕大鼠肝臟缺血再灌注損傷,能改善缺血再灌注損傷肝臟組織的能量代謝。
[Abstract]:Aim to observe the effects of sevoflurane and isoflurane preconditioning on hepatic ischemia-reperfusion injury and energy metabolism in rats. Methods Sixty SD male rats weighing 240 ~ 250g were randomly divided into three groups: hepatic ischemia reperfusion group, isoflurane preconditioning group, sevoflurane preconditioning group and sevoflurane preconditioning group. 30 min after hepatic ischemia, 30 min after hepatic ischemia and 30 min after liver reperfusion, the levels of serum alanine aminotransferase (alt), aspartate aminotransferase (AST) lactate dehydrogenase (LDH), tumor necrosis factor- 偽 (TNF- 偽), interleukin-1 尾 (IL-1 尾) and liver tissue in T3 were measured. The content of glutathione reductase GRN, malondialdehyde (MDA), The activity of superoxide dismutase (SOD) and the content of ATP in liver were collected for HE pathological staining. Group Sev: sealed container connected with the volatile tank of the corresponding anesthetic gas to monitor the concentration of sevoflurane in the sealed container. When the concentration of sevoflurane was stable at 2%, the rats were placed in an airtight container for 30 minutes and then dried in the air for 10 minutes. The remaining steps were the same as IR group and Iso group: an airtight container connected with the corresponding anesthetic gas volatile tank. The concentration of isoflurane in the closed vessel was monitored. When the concentration of isoflurane was stable at 1.5, the rats were placed in the closed container for 30 minutes, then the rats were taken out to air for 10 minutes. The remaining steps were the same as that in the IR group. Results Serum alt ASTT LDH was significantly increased at T _ 2 / T _ T _ 3 in IR group, Sev group and Iso group, compared with T _ 1 time point, respectively. There was no significant difference in serum alt ASTT LDH between Iso group and Iso group, compared with IR group. There was no significant difference in serum alt ASTT LDH between P0 05 sev group and Iso group compared with IR group at T0. 05 and T 0. 05%, respectively, compared with IR group, the serum alt AST LDH was significantly decreased at T 2 / T 3 time point in T _ 2 / T _ 2 / T _ 3 group, compared with that in T _ 2 / T _ 2 / T _ 3 group, and no significant difference was found between the two groups. There was a significant difference in serum TNF- 偽 and IL-1 尾 between the two groups (P 0.05 / IR) and Sev / Iso (P < 0.05), compared with that at T _ (1) and T _ (2) / T _ (3), respectively. The serum levels of interleukin-1 尾 (IL ~ (- 1)) were significantly decreased at T+ _ 2T _ (2) T _ (3) compared with those in IR group (P _ (0.05)) and T _ (2) T _ (+ +) (P _ (0.05) P _ (0.05)). There was no significant difference in serum IL-1 尾 between the two groups at T _ 2T _ 3. There was no significant difference in serum IL-1 尾 between the two groups. There was no significant difference in serum TNF- 偽 between the two groups at T _ 2T _ 3 and T _ 2T _ 3 compared with the IR group, respectively. There was no significant difference in serum TNF- 偽 between the Iso group and the IR group at the time of T _ 2T _ 3. MDA in liver tissue was significantly lower than that in T _ 3. Compared with IR group, the GR of liver tissue in Iso group was significantly higher than that in IR group, and the content of ATP in liver tissue of P0.05Sev group was significantly higher than that of IR group at T3 time point. The difference was statistically significant (P 0.05), and there was no significant difference between the two groups in the pathological changes of the liver and the corresponding changes of ischemia in the Sev group and the Iso group. Conclusion Pretreatment with sevoflurane and isoflurane can attenuate the hepatic ischemia-reperfusion injury and improve the energy metabolism of the liver.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R363

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