本文選題：脊髓　切入點(diǎn)：神經(jīng)前體細(xì)胞　出處：《南昌大學(xué)》2008年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 目的建立特異性表達(dá)巢蛋白(nestin)的轉(zhuǎn)基因小鼠動(dòng)物模型,觀察和分析成年動(dòng)物脊髓各個(gè)部位表達(dá)巢蛋白的陽(yáng)性細(xì)胞數(shù),探討神經(jīng)前體細(xì)胞(neural precursor cells ,NPCs)在脊髓各個(gè)部位的分布特征,為誘導(dǎo)自體神經(jīng)前體分化修復(fù)脊髓損傷的研究提供更多的理論依據(jù)。 方法采用巢蛋白第二內(nèi)含子增強(qiáng)子控制的LacZ(β-半乳糖苷酶)啟動(dòng)子轉(zhuǎn)基因小鼠(nestin second-intron enhancer controlled LacZ reporter transgenic mice, pNes-Tg)和LacZ染色及陽(yáng)性細(xì)胞量化技術(shù),觀察分析脊髓各個(gè)部位的巢蛋白表達(dá)陽(yáng)性細(xì)胞的分布特征,即表達(dá)部位和表達(dá)數(shù)量。 結(jié)果神經(jīng)前體細(xì)胞廣泛分布在成年小鼠的整個(gè)脊髓的各個(gè)部位,但是在不同區(qū)域其數(shù)量和密度不同,在脊髓后角分布最多,其次是中央管,前角和側(cè)角分布最少。在脊髓后角區(qū)神經(jīng)前體細(xì)胞主要分布在膠狀物質(zhì),在中央管神經(jīng)前體細(xì)胞主要分布在室管膜帶,在脊髓髓質(zhì)(白質(zhì))區(qū)和神經(jīng)核團(tuán)分布很少,灰質(zhì)比白質(zhì)分布多,左右兩邊分布無(wú)明顯差別。神經(jīng)前體細(xì)胞在脊髓的各個(gè)節(jié)段中分布有顯著差異,在頸段的分布最多,其次是腰骶段,在胸節(jié)段最少。但是同一節(jié)段(C1-7, T1-12, L1-5和骶段)的神經(jīng)前體細(xì)胞分布沒(méi)有顯著差異。 結(jié)論在脊髓中神經(jīng)前體細(xì)胞的分布特征可能與臨床脊髓的損傷和修復(fù)有相關(guān)性,神經(jīng)前體細(xì)胞分布較少的區(qū)域易于損傷且難于修復(fù),像前角、胸節(jié)段,相反,在后角、中央管神經(jīng)前體細(xì)胞分布最多,這些區(qū)域臨床較少發(fā)生損傷,且較易修復(fù)。神經(jīng)前體細(xì)胞在神經(jīng)元分布區(qū)域分布較少,而在神經(jīng)膠質(zhì)細(xì)胞分布區(qū)域和室管膜帶分布較多,這也提示神經(jīng)前體細(xì)胞的衍化與神經(jīng)膠質(zhì)細(xì)胞和室管膜細(xì)胞有密切關(guān)系。
[Abstract]:Objective to establish a transgenic mouse model with specific expression of nestin (nestin), observe and analyze the number of positive cells expressing nestin in various parts of spinal cord of adult animals, and investigate the distribution characteristics of neural precursor cells (NPCs) in different parts of spinal cord of adult animals. To provide more theoretical basis for the study of inducing autologous neural precursor differentiation to repair spinal cord injury. Methods the nestin second-intron enhancer controlled LacZ reporter transgenic mice( pNes-Tg) and LacZ staining and positive cell quantification technique were used in the transgenic mice controlled by the second intron enhancer of nestin (尾 -galactosidase). The distribution of nestin positive cells in different parts of spinal cord was observed and analyzed. Results nerve precursor cells were widely distributed in all parts of the spinal cord of adult mice, but their numbers and densities were different in different regions, and most of them were in the posterior horn of spinal cord, followed by the central canal. The distribution of anterior horn and lateral horn was the least. In the posterior horn of spinal cord, nerve precursor cells were mainly distributed in colloid substance, in central canal nerve precursor cells were mainly distributed in ependymal zone, and in medullary matter (white matter) and nucleus of spinal cord. The distribution of nerve precursor cells in each segment of the spinal cord was significantly different, and the distribution in the cervical segment was the most, followed by the lumbosacral segment, the distribution of the gray matter was more than that of the white matter, and there was no significant difference between the left and right sides of the distribution. But there was no significant difference in the distribution of neural progenitor cells between C1-7, T1-12, L1-5 and sacral segments in the same segment. Conclusion the distribution of neural precursor cells in the spinal cord may be related to the injury and repair of the clinical spinal cord. Areas with less distribution of neural precursor cells are prone to injury and difficult to repair, such as the anterior horn, thoracic segment, and, on the contrary, in the posterior horn. The nerve precursor cells in the central duct were most distributed, which were less damaged in clinic and easier to repair. The neural progenitor cells were less distributed in the neuronal distribution area, but more in the glial cell distribution area and ependymal zone. This also suggests that the evolution of neural precursor cells is closely related to glial cells and ependymal cells.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類(lèi)號(hào)】：R329

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