本文選題：肌衛(wèi)星細(xì)胞　切入點(diǎn)：成肌細(xì)胞　出處：《中國(guó)協(xié)和醫(yī)科大學(xué)》2008年碩士論文　論文類型：學(xué)位論文

【摘要】： 骨骼肌組織起源于中胚層,成體骨骼肌組織由終末分化的多核肌纖維和幾種單個(gè)核細(xì)胞群組成,其中單個(gè)核細(xì)胞群體被認(rèn)為是骨骼肌組織中處于原始分化狀態(tài)的干細(xì)胞。這些骨骼肌干細(xì)胞在體內(nèi)多數(shù)情況下呈一種靜息狀態(tài),當(dāng)肌肉受到各種損傷因素等刺激時(shí)被激活,大量增殖、融合,分化形成新的成熟的骨骼肌細(xì)胞,從而修復(fù)受損組織。這些細(xì)胞在體外培養(yǎng)基和營(yíng)養(yǎng)因子的作用下,也可被激活并大量增殖,此時(shí)體外培養(yǎng)的激活的骨骼肌干細(xì)胞被稱為成肌細(xì)胞。 一些研究已經(jīng)表明,骨骼肌干細(xì)胞同其他組織的干細(xì)胞同樣具有潛在的多向分化能力,它們除了向骨骼肌組織分化以外,還可在特定條件下多潛能分化形成中胚層來(lái)源的成骨組織、脂肪樣細(xì)胞、間質(zhì)細(xì)胞,并可跨越胚層譜系的限制向神經(jīng)外胚層轉(zhuǎn)化,從而可被用來(lái)做為細(xì)胞來(lái)源,進(jìn)行神經(jīng)系統(tǒng)疾病的移植治療。細(xì)胞來(lái)源,進(jìn)行神經(jīng)系統(tǒng)疾病的移植治療。 本研究通過(guò)聯(lián)合應(yīng)用特殊培養(yǎng)基和細(xì)胞因子,體外誘導(dǎo)骨骼肌組織來(lái)源的干細(xì)胞(即成肌細(xì)胞)向神經(jīng)外胚層的原始細(xì)胞-神經(jīng)干細(xì)胞轉(zhuǎn)化,并進(jìn)一步向神經(jīng)元和膠質(zhì)細(xì)胞分化;同時(shí)與成肌細(xì)胞系和非肌肉組織的細(xì)胞系進(jìn)行比較,證明誘導(dǎo)條件對(duì)原代培養(yǎng)的成肌細(xì)胞具向神經(jīng)外胚層的轉(zhuǎn)化具有特異性。實(shí)驗(yàn)結(jié)果提示骨骼肌來(lái)源的干細(xì)胞有可能作為治療神經(jīng)系統(tǒng)疾病尤其是神經(jīng)退行性疾病的一種新的干細(xì)胞來(lái)源。
[Abstract]:Skeletal muscle tissue originates from mesoderm and adult skeletal muscle tissue is composed of terminally differentiated polynuclear muscle fibers and several mononuclear cell groups. The mononuclear cell population is thought to be the primary differentiated stem cell in skeletal muscle tissue. These skeletal muscle stem cells are in a resting state in most cases, activated when the muscle is stimulated by a variety of injury factors. They proliferate, fuse, and differentiate into new mature skeletal muscle cells that repair damaged tissue. These cells can also be activated and proliferated in vitro under the influence of nutrient factors. Activated skeletal muscle stem cells in vitro are called myoblasts. Some studies have shown that skeletal muscle stem cells have the same potential for multiple differentiation as stem cells from other tissues, in addition to differentiating into skeletal muscle tissues. They can also differentiate into mesoderm-derived osteoblasts, aliphatic cells, mesenchymal cells under certain conditions, and can be transformed to the neuroectodermal layer across the limits of the epiderm lineage, so that they can be used as a source of cells. Transplant treatment of nervous system diseases. Cell source, transplantation treatment of nervous system diseases. In this study, we used special culture medium and cytokines to induce the transformation of skeletal muscle derived stem cells (myoblasts) into neural stem cells (NSCs) in neuroectodermal layer. And further differentiated into neurons and glial cells, and compared with myoblasts and non-muscle cell lines, The results show that the induced conditions are specific to the transformation of primary cultured myoblasts into neuroectodermal layer. The results suggest that the stem cells derived from skeletal muscle may be used to treat nervous system diseases, especially neurodegenerative diseases. A new source of stem cells.
【學(xué)位授予單位】：中國(guó)協(xié)和醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 郭雨霽,高英茂,邴魯軍;紋狀體組織塊誘導(dǎo)胚胎干細(xì)胞定向分化的實(shí)驗(yàn)研究[J];山東大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2003年02期

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本文編號(hào)：1557037