本文關(guān)鍵詞： 葡萄糖代謝 雙組分信號(hào)轉(zhuǎn)導(dǎo)系統(tǒng)saeRS 生物膜形成 表皮葡萄球菌　出處：《四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2015年01期 　論文類(lèi)型：期刊論文

【摘要】：目的研究不同濃度的葡萄糖對(duì)表皮葡萄球菌野生株、saeRS基因刪除突變株及回復(fù)株生存能力的影響及機(jī)制。方法在BM培養(yǎng)基中添加不同濃度(7.0mmol/L、14mmol/L、28mmol/L、56mmol/L)的葡萄糖,分析3種表皮葡萄球菌的生存能力、生物膜形成能力、培養(yǎng)基的酸度及抵抗抗生素生存的能力。結(jié)果與表皮葡萄球菌野生株相比,葡萄糖能明顯促進(jìn)saeRS刪除突變株的生存能力和生物膜形成能力,但能夠明顯降低其對(duì)抗生素(比如青霉素、苯唑西林、慶大霉素、環(huán)丙沙星和丁胺卡那霉素)的抵抗能力;saeRS刪除突變株和回復(fù)株在14mmol/L濃度時(shí)生存能力最強(qiáng),在28mmol/L濃度時(shí)生物膜形成能力最強(qiáng),而葡萄糖濃度對(duì)于野生株的生存能力及生物膜形成能力無(wú)顯著影響。在添加14mmol/L葡萄糖時(shí),saeRS刪除突變株培養(yǎng)基(pH=8.07)的酸度比野生株(pH=7.0)明顯降低。結(jié)論 SaeRS可能通過(guò)介導(dǎo)葡萄糖的利用影響了表皮葡萄球菌的生存能力;雙組分信號(hào)轉(zhuǎn)導(dǎo)系統(tǒng)saeRS影響抗生素如青霉素、苯唑西林、慶大霉素、環(huán)丙沙星和丁胺卡那霉素對(duì)表皮葡萄球菌的殺菌作用。
[Abstract]:Objective to study the effect and mechanism of glucose concentration on the survival ability of staphylococcus epidermidis wild strain (S.epidermidis) to delete the mutant gene and to recover the gene. Methods glucose of 7.0mmol / L ~ (14) mmol / L ~ (28) mmol / L ~ (28) mmol / L ~ (56) mmol 路L ~ (-1) was added to BM medium. The survival ability, biofilm forming ability, acidity of culture medium and survival ability of antibiotic resistance of three species of Staphylococcus epidermidis were analyzed. Results compared with the wild strains of Staphylococcus epidermidis, Glucose significantly increased the viability and biofilm formation of saeRS deletion mutants, but significantly reduced its resistance to antibiotics (such as penicillin, oxacillin, gentamicin, etc.). The resistance of ciprofloxacin and amikacin) was the strongest at 14 mmol / L and biofilm formation at 28 mmol / L. However, glucose concentration had no significant effect on the viability and biofilm formation ability of wild plants. The acidity of the culture medium containing 14mmol / L glucose was significantly lower than that of wild plants. Conclusion the pH value of SaeRS may be decreased by adding 14mmol / L glucose to the culture medium (pH = 8.07). Conclusion the results suggest that the concentration of glucose has no significant effect on the viability and biofilm formation of wild plants. The utilization of glucose conduction affected the viability of Staphylococcus epidermidis. Two-component signal transduction system (saeRS) affects the bactericidal effects of antibiotics such as penicillin, oxacillin, gentamicin, ciprofloxacin and amikacin on Staphylococcus epidermidis.
【作者單位】： 河南大學(xué)醫(yī)學(xué)院河南省醫(yī)學(xué)與分子免疫學(xué)實(shí)驗(yàn)室抗體藥物河南省工程實(shí)驗(yàn)室;復(fù)旦大學(xué)上海醫(yī)學(xué)院教育部/衛(wèi)生部醫(yī)學(xué)分子病毒學(xué)重點(diǎn)實(shí)驗(yàn)室;
【分類(lèi)號(hào)】：R378.1

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本文編號(hào)：1544973