發(fā)布時(shí)間：2018-02-23 20:36

  本文關(guān)鍵詞： 人髓核細(xì)胞 信號(hào)傳導(dǎo) 炎癥因子 P38MAPK JNK/SAPK 細(xì)胞凋亡　出處：《青島大學(xué)》2010年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 目的研究炎癥因子腫瘤壞死因子α(TNF-α)和白介素6(IL-6)對(duì)人髓核細(xì)胞凋亡的影響及可能機(jī)制。方法培養(yǎng)人髓核細(xì)胞,將細(xì)胞隨即分為7組：TNF-α刺激組；TNF-α+P38MAPK阻斷組；TNF-α+JNK/ SAPK阻斷組；IL-6刺激組；IL-6+P38MAPK阻斷組;IL-6+JNK/SAPK及對(duì)照組。然后用AnnexinⅤ/PI染色流式細(xì)胞儀和TUNEL法檢測(cè)髓核細(xì)胞凋亡情況,免疫熒光檢測(cè)P-P38MAPK和P-JNK/SAPK的表達(dá)及定位；Western印跡法檢測(cè)P38MAPK,JNK/SAPK及其磷酸化形式的表達(dá)。結(jié)果在流式細(xì)胞儀檢測(cè)凋亡結(jié)果中,TNF-α和IL-6刺激組相對(duì)于對(duì)照組及相應(yīng)阻斷組均有著較高的凋亡細(xì)胞密度(P＜0.01)；TUNEL法檢測(cè)凋亡結(jié)果中,TNF-α和IL-6刺激組相對(duì)于對(duì)照組及相應(yīng)阻斷組亦均有著較高的凋亡細(xì)胞密度(P0.01)；免疫熒光結(jié)果顯示TNF-α和IL-6刺激組P-P38MAPK和P-JNK/SAPK在細(xì)胞質(zhì)和細(xì)胞核均有表達(dá),各阻斷組在細(xì)胞質(zhì)和細(xì)胞核可見(jiàn)少量表達(dá),對(duì)照組僅見(jiàn)極少量表達(dá),TNF-α和IL-6刺激組差異和相應(yīng)阻斷組及對(duì)照組間差異有顯著統(tǒng)計(jì)學(xué)意義(P＜0.01),Western印跡法結(jié)果顯示P38MAPK,JNK/SAPK在各組髓核細(xì)胞內(nèi)均有表達(dá),但無(wú)活化形式,TNF-α和IL-6刺激組可見(jiàn)P-P38MAPK,P-JNK/SAPK表達(dá),但相應(yīng)阻斷組無(wú)表達(dá)。結(jié)論外源性TNF-α和IL-6可通過(guò)激活P38MAPK和JNK/SAPK途徑導(dǎo)致人髓核細(xì)胞凋亡。
[Abstract]:Objective to study the effect of inflammatory factor TNF- 偽 (TNF- 偽) and interleukin-6 (IL-6) on apoptosis of human nucleus pulposus cells. Methods Human nucleus pulposus cells were cultured. The cells were then divided into 7 groups: TNF- 偽 P38MAPK blocking group (TNF- 偽 P38 MAPK blocking group), TNF- 偽 JNK/SAPK blocking group (TNF- 偽 JNK/SAPK blocking group), IL-6 P38 MAPK blocking group (IL-6 JNK/SAPK) and control group (control group). The apoptosis of nucleus pulposus cells was detected by Annexin 鈪，

本文編號(hào)：1527527